Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913445,5580AC27.9% 219.8 / 62.1 122V370G (GTA→GGA) yajRputative major facilitator superfamily transport protein YajR
Reads supporting (aligned to +/- strand):  ref base A (61/27);  new base C (0/34);  total (61/61)
Fisher's exact test for biased strand distribution p-value = 1.75e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 175 total aligned reads displayed.

AGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGG  >  NC_000913/445420‑445696
                                                                                                                                          |                                                                                                                                           
aGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATAc                                                                                                                                            <  2:1877881/140‑1 (MQ=255)
aGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATAc                                                                                                                                            <  2:1829945/140‑1 (MQ=255)
     gcGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGTGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                       <  1:1702565/139‑1 (MQ=255)
     aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATAcccccc                                                                                                                                       >  1:142977/1‑139 (MQ=255)
     aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                       <  2:227316/140‑1 (MQ=255)
     aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                       <  1:985212/140‑1 (MQ=255)
     aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                       <  1:1610541/140‑1 (MQ=255)
       gCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGa                                                                                                                                     <  2:1100222/140‑1 (MQ=255)
           ttttAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGt                                                                                                                                 <  2:1742445/140‑1 (MQ=255)
               aaCGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAAAACCCCCTGACCGTC‑aaa                                                                                                                            >  1:1439526/1‑140 (MQ=255)
                 cGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACa                                                                                                                          <  2:684097/140‑1 (MQ=255)
                 cGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGGACTGGCGACCGTCAGCCACACTGTGGCCCGCATTGCGCCCGCGAGAAAAACCCCCCGACCCTC‑AAAcc                                                                                                                          >  2:1702565/1‑139 (MQ=255)
                  gCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACAt                                                                                                                         <  2:1529166/140‑1 (MQ=255)
                  cccTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACAt                                                                                                                         <  2:1592571/139‑1 (MQ=255)
                     tCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGGACTGGCGACCGTCAGCCCCACTGCGGCCAGCATTTCGCCAGCGAGGAAAACCCCCCGACCGTC‑CAAAAAGcc                                                                                                                      >  2:971113/1‑140 (MQ=255)
                       gTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGt                                                                                                                    >  1:109220/1‑140 (MQ=255)
                               cAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAg                                                                                                            <  2:785960/140‑1 (MQ=255)
                                 aTGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACCGCCGCCCGCATTTCGCCAGCGAGAAAAACCCCCCGACCGTC‑AAAAAATCCGTTAATCCCgcc                                                                                                          >  2:2138134/1‑140 (MQ=255)
                                   gTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAgccgc                                                                                                        <  2:1439526/140‑1 (MQ=255)
                                           ggATTTAAATGCGCAAACTGCTGAAATACGGCGTTTCTTTCATGGTACGGGCGACTGTCAGCCACCCTGCGGCCAGCTTTGCGCCAGAGAGAAATCCCCCCTGCCCGAA‑AAACATGCCGTTAATCCAGCCGCCCAGCGaa                                                                                                <  2:1641250/140‑1 (MQ=255)
                                            gATTCCAATGCGCAACCTGCTGACATCCGGGGGTTCTTTCATGGTACTGGCGACTGTCAGCCACCCTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAAc                                                                                               <  2:244309/140‑1 (MQ=255)
                                                  aaTGCGCAAACTGCTGACATACGGCGGTTCTTTTATGGTACTTGCGGCTGTCAACCACACTGCGGCCCGCAATGCGCCCGCGAGAAAAACCCCCCTACCGTC‑AAACATGCCGTTAAACCAGCCGCCCAGCGAACCCCCaa                                                                                         >  2:134165/1‑140 (MQ=255)
                                                      cgcAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGCA‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgc                                                                                     <  2:2228245/140‑1 (MQ=255)
                                                      cgcAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCCGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCCATcgc                                                                                     >  1:1011709/1‑140 (MQ=255)
                                                        cAAACTGCTGACATACGGCGGTTCTTTTATGGTACTTGCGACTGTCAGCCCCACCGCGGCCCGCATTGCGCCCGCGAGAAAAAACCCCTGACCGTC‑AAAAATGCCGGTAATCCCACCCCCCCCCGAACCGCCAATccccc                                                                                   >  2:116321/1‑139 (MQ=255)
                                                        aaaccggctgACATAGGGGGTTTCTTCCAGGGTACGGGCGACTGTCAGCCACACGGGGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGCCCGTA‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgcca                                                                                   <  2:1065975/134‑1 (MQ=255)
                                                                    aTACGGCGTTTCTTTCAGGGTCCGGGCGACTGTCACCCACACTGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAAct                                                                       <  2:387300/140‑1 (MQ=255)
                                                                      aCGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTC‑AAACATACCGTTAATCCAACCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAActgg                                                                     <  2:599125/140‑1 (MQ=255)
                                                                       gggcggTTCTTTCATGGTACGGGCGACTGTCAGCCACCCTGCGGCCAGCATGGCGCCGGCGAAAAATCCCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAActggc                                                                    <  2:142977/139‑1 (MQ=255)
                                                                       cgggggTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCGGCGAGAAATCCCCCCTAACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAActggc                                                                    <  2:135145/140‑1 (MQ=255)
                                                                       cggcggTTCTTTCATGGTCCTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCACCACGCCAAGAAActggc                                                                    <  1:1503924/140‑1 (MQ=255)
                                                                       cggcggTTCTTTCATGGTACTGGGGACCGTCAGCCACACTGCGGCCCGCATTGCGCCAGCGGGGAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCCCCCCGCGAACCGCCCATCGCCACGCCAAAAAAatggc                                                                    >  2:907589/1‑140 (MQ=255)
                                                                       cggcggTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTTCGCCAGCGAGAAGTACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCCGCGAACCGCCAATCGCCACGCCAAGAAActggc                                                                    >  2:273962/1‑140 (MQ=255)
                                                                         gcgtTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTA‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAActggctg                                                                  <  2:406255/140‑1 (MQ=255)
                                                                         gcggTTTTTTCATGGTACTGGCGACCGTCAGCCACACTGCGGCCAGCATTGGGGCAGCGAGAAAAACCCCCCGACCGGC‑AAACATGCCCTTTAACCAGCCGCCCAGCCAACCGCCCATCCCCCCGCCCAAAAAccggccg                                                                  >  2:106001/1‑138 (MQ=255)
                                                                         gcggTTCTTTTATGGTACTTGCGACTGTCAGCCCCACTGCGGGCAGCATTTCGCCAGCGGGAAATAACCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCCAACACCACCCCCAGAAAatggctg                                                                  >  2:1503924/1‑140 (MQ=255)
                                                                         gcggTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAActggctg                                                                  <  2:775003/140‑1 (MQ=255)
                                                                           ggTTCTTTCATGGTACTGGCGACTGTCACCCACACTGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggt                                                                <  2:1307038/140‑1 (MQ=255)
                                                                              tCTTTCATGGTACTGGCGACTGTCAGCCACACTTCCGCCAGCATTTCGCCAGCGAGAAAAACCCCCCGACCGCC‑AAACATGCCGGTAATCCAGCCCCCCCGCGAACCGCCAATCGCCACGCCAAGAAACACGCtgggggg                                                             >  2:1906631/1‑139 (MQ=255)
                                                                              tCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGa                                                             <  1:2282608/140‑1 (MQ=255)
                                                                                       gTACTGGCGACTGTCAGCCACACTGCGGTCAGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGCC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAcc                                                    <  1:660964/140‑1 (MQ=255)
                                                                                       gTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAcc                                                    >  2:1596262/1‑140 (MQ=255)
                                                                                       gTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAcc                                                    >  1:1889026/1‑140 (MQ=255)
                                                                                        tACTGGCGTCTGTCACCCACCCGGCGGCCAGCATTGCGCAAGCGAGAAATCCCCCCTGACCGTA‑AAACATGCCGTTAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAccc                                                   <  1:167260/140‑1 (MQ=255)
                                                                                        tACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGTA‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAccc                                                   <  1:145915/140‑1 (MQ=255)
                                                                                         aCTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCa                                                  >  2:1987841/1‑140 (MQ=255)
                                                                                          cTGGCGCCTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCAt                                                 <  1:2022999/140‑1 (MQ=255)
                                                                                          cTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCAt                                                 >  1:369163/1‑140 (MQ=255)
                                                                                          cTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCAt                                                 >  2:169759/1‑140 (MQ=255)
                                                                                            ggCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCACGAAACTGGCTGGTGGAGTAAACACCCATCg                                               >  2:2004581/1‑140 (MQ=255)
                                                                                                aCTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGt                                           <  1:1600947/140‑1 (MQ=255)
                                                                                                aCTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCAAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGt                                           <  1:2371089/140‑1 (MQ=255)
                                                                                                 gggTCACCCACCCTGCGGCCAGCTTTGCGCCAGCGAGAAATCCCCCCTGCCCGTA‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                          <  2:1876153/138‑1 (MQ=255)
                                                                                                 ctcccAGCCACACTGGGGCCAGCTTGCCGCCAGGGAAAAATCCCCCCTGCCCCTA‑AAACATGCCTTTAATCCAGCCCCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                          <  2:1662474/136‑1 (MQ=255)
                                                                                                 cTGTCAGCCACCCGGCGGCCAGCATGGCGCCAGCGAAAAATCCCCCCTGCCCGCC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                          <  1:1539921/140‑1 (MQ=255)
                                                                                                 cTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                          <  1:369797/140‑1 (MQ=255)
                                                                                                 cTGTCAGCCACACTCGGCCCAGCATTGCGCAAGCAAGAAATCCCCCCTGACCGTC‑AACAATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                          <  1:2194271/140‑1 (MQ=255)
                                                                                                  tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAccc                                                   <  1:1166290/130‑1 (MQ=255)
                                                                                                  tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAccc                                                   >  2:1166290/1‑130 (MQ=255)
                                                                                                  tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                         >  2:1018208/1‑140 (MQ=255)
                                                                                                  tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                         >  2:202525/1‑140 (MQ=255)
                                                                                                  tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                         >  2:2319343/1‑140 (MQ=255)
                                                                                                  tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                         >  2:1208044/1‑140 (MQ=255)
                                                                                                  tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                         >  1:247954/1‑140 (MQ=255)
                                                                                                  tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                         >  2:308210/1‑140 (MQ=255)
                                                                                                  tGTCAGCCACACTGCGGCCAGCATTGCGACAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                         >  2:931436/1‑140 (MQ=255)
                                                                                                    tCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCt                                       >  1:193695/1‑140 (MQ=255)
                                                                                                         cacaCTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTa                                  <  1:1208044/140‑1 (MQ=255)
                                                                                                         cacaCTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTa                                  >  1:1050998/1‑140 (MQ=255)
                                                                                                            acTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAAcc                               >  1:602046/1‑140 (MQ=255)
                                                                                                              tGCGGCCAGCATTGCGCCAGCGAGAAGTACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                             >  1:1499002/1‑140 (MQ=255)
                                                                                                              tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCGGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                             >  1:1261127/1‑140 (MQ=255)
                                                                                                                cgaccAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctgct                           >  1:2196148/4‑140 (MQ=255)
                                                                                                                cGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctgct                           >  1:1976288/1‑140 (MQ=255)
                                                                                                                  gCCAGCTTGGCGCCAGCGAAAAATCCCCCCTGCCCGTA‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                         <  2:1944293/140‑1 (MQ=255)
                                                                                                                  gCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTA‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                         <  1:77999/140‑1 (MQ=255)
                                                                                                                  gCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                         >  2:1359782/1‑140 (MQ=255)
                                                                                                                  gCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                         >  1:1394906/1‑140 (MQ=255)
                                                                                                                   ccAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGc                        >  2:2114674/1‑140 (MQ=255)
                                                                                                                   ccAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGc                        >  1:1546232/1‑140 (MQ=255)
                                                                                                                    cAGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCg                       <  1:282492/140‑1 (MQ=255)
                                                                                                                     agctgtGCGCCAGGGA‑AAAAACCCCCTTGACGTCAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                      <  2:1858663/135‑1 (MQ=255)
                                                                                                                     aGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                      <  1:23563/140‑1 (MQ=255)
                                                                                                                     aGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                      <  1:907589/140‑1 (MQ=255)
                                                                                                                     aGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                      <  1:184799/140‑1 (MQ=255)
                                                                                                                     aGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                      <  1:12160/140‑1 (MQ=255)
                                                                                                                     aGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                      <  1:1359782/140‑1 (MQ=255)
                                                                                                                     aGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                      >  1:2141001/1‑140 (MQ=255)
                                                                                                                      gCATGGCGCCAGCGAAAAATCCCCCCTGCCCGTA‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAc                     <  2:1584032/140‑1 (MQ=255)
                                                                                                                      ccATGGCGCCGGCAAAAAATCCCCCCTACCCGCA‑AAACATGCCTTTAATCCAGCCGCCCAGCAAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAc                     <  2:1157546/139‑1 (MQ=255)
                                                                                                                       catgccgcCAGCGAAAAATCCCCCCTAACCTTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAct                    <  2:193695/135‑1 (MQ=255)
                                                                                                                       cATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAct                    >  1:854713/1‑140 (MQ=255)
                                                                                                                         ttGCGCCAGCGAGAAATCCCCCCTGCCCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct                  <  2:323274/140‑1 (MQ=255)
                                                                                                                         ttGCGCCAGCGAGAAATCCCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct                  <  1:237063/140‑1 (MQ=255)
                                                                                                                           gcgcCGGCGAAAAATCCCCCCTGCCCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt                <  1:454462/140‑1 (MQ=255)
                                                                                                                           gcgcCAGCGAGAAATCCCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCGGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt                <  1:80126/140‑1 (MQ=255)
                                                                                                                                 gCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt          >  2:449612/1‑140 (MQ=255)
                                                                                                                                     aaaaTCCCCCCTGCCCGTA‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGt      <  1:2082677/139‑1 (MQ=255)
                                                                                                                                     aaaaTACCCACTGACCGTC‑AACCATGCCGTAAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGt      <  1:547/139‑1 (MQ=255)
                                                                                                                                       aaTCCCCCCTGCCCGTA‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGa    <  1:134165/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
AGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTC‑AAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGG  >  NC_000913/445420‑445696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑