| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 650,809 | 0 | T | G | 23.6% | 187.0 / 19.1 | 89 | T350P (ACC→CCC) | citC | citrate lyase synthetase |
| Reads supporting (aligned to +/- strand): ref base T (22/46); new base G (21/0); total (43/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.08e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGATA > NC_000913/650672‑650946 | cAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCt > 1:553913/1‑140 (MQ=255) gctgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTg < 2:473364/140‑1 (MQ=255) ctgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGa > 2:431948/1‑140 (MQ=255) ctgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGGTTTTTTTTATTTTTTACCTGTTTTTCATGCGGGGGGCTTTTgt > 2:730525/1‑139 (MQ=255) tgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGGTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTTAc > 1:716882/1‑140 (MQ=255) ctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGGTGGTTTATTTTCATTTTTTACCTGTTTCTCATGCGGGGGGCTTTTGACGa > 2:887140/1‑140 (MQ=255) ctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGGTTATTTTTATTTTTTACCTGTTTCTCATGCGGGGGTCTTTTGACGa > 2:132404/1‑140 (MQ=255) ctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGATTATTTTTTTTTTTCTCCTGTTTTTCTTGCGGGGGTCTTTTGACGa > 2:209535/1‑140 (MQ=255) tATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAg < 1:490184/140‑1 (MQ=255) gATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGGTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTTACGAGCTGc > 2:1081741/1‑140 (MQ=255) aTTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGcc < 2:717119/140‑1 (MQ=255) aTTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGcc < 2:1119747/140‑1 (MQ=255) ttGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCcgc > 2:867776/1‑140 (MQ=255) gCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGt < 1:730525/140‑1 (MQ=255) gCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGt < 1:927888/140‑1 (MQ=255) gCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGt < 1:403218/140‑1 (MQ=255) cGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGGTTTTTTTGATTTTTTCCCCGTTTCTCCTGCGGGGGGCTTTTGACGAACCGGCGCGcccctgc > 2:343464/1‑136 (MQ=255) atatCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCgg < 2:940952/140‑1 (MQ=255) tatCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCggg < 1:431948/140‑1 (MQ=255) tatCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCggg < 2:553913/140‑1 (MQ=255) atCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGa < 2:913722/140‑1 (MQ=255) atCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGa < 1:343464/140‑1 (MQ=255) atCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGa < 2:641743/140‑1 (MQ=255) tCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGGCGGGTTTTTTTTC‑TTTTTTACCCGTTTTTCCTGCGGGGGGCTTTTTACGAGCTGCCGCGTCCCGGCggggg > 2:516620/1‑138 (MQ=255) ccTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGt < 1:670548/140‑1 (MQ=255) cTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTg < 2:1010323/140‑1 (MQ=255) cTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTg < 2:665263/140‑1 (MQ=255) tGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGc > 1:589541/1‑140 (MQ=255) gCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTCTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCt > 1:520098/1‑140 (MQ=255) gCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGGTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGGGcc > 1:213053/1‑139 (MQ=255) cGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTTATTTTTTACCTGTTTTTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGGGGGCTc > 2:529584/1‑140 (MQ=255) cGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCggg < 2:95229/133‑1 (MQ=255) cGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCggg > 1:95229/1‑133 (MQ=255) aTCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCCGTTTTTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGGGGGGTCAAg > 2:1072157/1‑140 (MQ=255) tCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGc < 1:393804/140‑1 (MQ=255) cGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCa < 2:553904/140‑1 (MQ=255) cGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGGTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGGTCAAACa > 2:129898/1‑140 (MQ=255) gAGTGGGGCGATGCGTATCATCACATCGCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑g < 1:708077/140‑1 (MQ=255) aGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑gg < 1:95208/140‑1 (MQ=255) gggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCt < 1:1177461/140‑1 (MQ=255) gggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCt < 1:529584/140‑1 (MQ=255) ggCGATGCGTATCATCACATCGCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTTACCTGTTTCTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGGGGGGTCAAGCA‑GGGTCtt > 2:708077/1‑139 (MQ=255) ggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTTACCTGTTTTTCATGCGGGGGTCTTTTGACGAGCTGCCGCGGCCTGGCGGGGGGGCTCCAGCA‑gggtttt > 2:108836/1‑135 (MQ=255) gCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGc < 1:1191492/140‑1 (MQ=255) gCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGc < 1:784416/140‑1 (MQ=255) gCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGc < 1:839490/140‑1 (MQ=255) gCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGc < 1:953337/140‑1 (MQ=255) aTGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCaaa < 1:1072157/140‑1 (MQ=255) tGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTTTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCCGGCGGGGGGGCTCCAACA‑GGGTCTTCaaaa > 2:95208/1‑139 (MQ=255) tGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGGGTGCTCAAGCA‑GGGTCTGCaaaa > 2:85025/1‑139 (MQ=255) tATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATg < 2:1108747/140‑1 (MQ=255) atcatcACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAACA‑GGGTTTGCAaaaaattc > 1:1069033/1‑138 (MQ=255) cacaTCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGt < 1:108836/140‑1 (MQ=255) caTCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTTTTTTCATTTTTTACCCGTTTTTCCTGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGGCCCAGCC‑GGGTCTTCAAAAAATGCCGCGTgg > 2:1037573/1‑139 (MQ=255) caTCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTTTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAAGA‑GGGTCTGGAAATAATTCAGCgggg > 1:581052/1‑137 (MQ=255) caTCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGa < 1:661834/140‑1 (MQ=255) caTCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGa < 1:281448/140‑1 (MQ=255) tCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGGTGGGTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTTACGAGCTGCCGCGTCCTGGCGGGGGGGGTCAAACA‑GGGTTTGCAAAAAAAGCAGCGGGACt > 2:281448/1‑140 (MQ=255) tCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTTAACTGTTTCTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGGGGGGTCCAGCAGGGTTTTGCAAAAAATGCacggggct > 2:903897/1‑133 (MQ=255) aGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGTGCTCAAGCA‑AGTTTTTCAAATAATGCAGCGGGACTGCAgg > 1:481736/1‑140 (MQ=255) gACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGTGCTCAAGCA‑GGGTCTGCAAAAAAAGCAGCGTGACTGCAggg > 1:198612/1‑140 (MQ=255) aaGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAg < 2:633274/140‑1 (MQ=255) aaGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGGGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAg > 1:347648/1‑140 (MQ=255) aGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAgc < 1:801422/140‑1 (MQ=255) aGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGGGCTCAAACA‑GGGTCTGCAAATAATGCAGCGTGACTGCAGGGACCAgc > 1:446079/1‑140 (MQ=255) gggTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAgcg < 1:867776/140‑1 (MQ=255) gggTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCCGGGGGGCCCCAACA‑AGGTCTGCAAAAAATGCAGCGGGAATGAAGGGaaccccg > 2:506036/1‑134 (MQ=255) tGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAgcggcg < 1:233590/140‑1 (MQ=255) gCCTGCAACGGC‑GGGCTGGTTTTTTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGTGCTCAAACA‑GGGTTTGCAAATAATGCAGCGGGGCTGCAGGGGCCAGCGgcgc > 2:20388/1‑140 (MQ=255) gCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGCGACTGCAGGGACCAGCGgcgc > 1:822245/1‑140 (MQ=255) gCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGGGCTCAAACA‑GGTTTTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGgcgc > 1:456177/1‑140 (MQ=255) gCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGGGTGGTCAAACA‑GGGTTTGCAAATAAAGCAGCGGGACTGCAGGGGGCAGCGgggc > 2:424600/1‑140 (MQ=255) gCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGGGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAgcggcgt > 1:676726/1‑139 (MQ=255) ccTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGGTCAAGCA‑GGGTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGcgcg > 1:74231/1‑140 (MQ=255) ccTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCCAGCA‑GGGTTTGCAAATAATGCAGCGGGGCTGCAGGGGCCCGCGGcgcg > 2:145575/1‑140 (MQ=255) ccTGCAACGGC‑GGGCTGGGTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCCAGCA‑GGGTTTTCCAAAAATGCAGCGTGACTGCGGGGGCCCgcgggggg > 2:167496/1‑136 (MQ=255) ggc‑ggGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGt < 1:506036/140‑1 (MQ=255) gCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTCTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGAt < 2:520098/140‑1 (MQ=255) ttCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGcc < 2:589541/140‑1 (MQ=255) ttCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGcc < 2:260668/140‑1 (MQ=255) ttCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGAGATAGCCGTGAGATCGTTTTTCGcc < 1:412084/140‑1 (MQ=255) aTTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCcagc > 1:340470/1‑140 (MQ=255) ttttCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCcagcag < 1:223255/140‑1 (MQ=255) tCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTg > 2:864983/1‑140 (MQ=255) tGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGGCGTa > 1:712252/1‑140 (MQ=255) tttCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTAcc > 2:806244/1‑140 (MQ=255) tttCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTAcc > 2:493143/1‑140 (MQ=255) ctcATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCgg < 1:315449/140‑1 (MQ=255) ctcATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGCGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCgg < 2:822245/140‑1 (MQ=255) gggggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCgg > 1:384916/1‑140 (MQ=255) gggggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCgg > 2:780311/1‑140 (MQ=255) gggggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCgg > 1:951772/1‑140 (MQ=255) tgggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGa > 1:825360/2‑140 (MQ=255) ggggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCGAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGa > 1:776158/1‑123 (MQ=255) ggggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCGAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGa < 2:776158/123‑1 (MQ=255) ggggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGa > 1:164587/1‑140 (MQ=255) gggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGat > 2:916615/1‑140 (MQ=255) ggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGata < 1:760682/140‑1 (MQ=255) | CAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGC‑GGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGATA > NC_000913/650672‑650946 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |