Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,621,1180AC24.4% 77.4 / 11.8 41V7G (GTG→GGG) eamAcysteine/O‑acetylserine exporter EamA
Reads supporting (aligned to +/- strand):  ref base A (21/10);  new base C (0/10);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 1.65e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCT  >  NC_000913/1620982‑1621254
                                                                                                                                        |                                                                                                                                        
tGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACAccc                                                                                                                                       >  1:159418/1‑140 (MQ=255)
  cGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCa                                                                                                                                     >  1:283283/1‑140 (MQ=255)
   gACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCAt                                                                                                                                    <  2:1150818/140‑1 (MQ=255)
     cAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCt                                                                                                                                  >  2:570049/1‑140 (MQ=255)
       aaaaaGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCttt                                                                                                                                <  1:570049/140‑1 (MQ=255)
          aaGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTcg                                                                                                                             <  1:1067851/140‑1 (MQ=255)
          aaGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTcg                                                                                                                             <  2:283283/140‑1 (MQ=255)
            gATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTcgcg                                                                                                                           <  2:729558/140‑1 (MQ=255)
             aTAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGa                                                                                                                          <  1:1190668/140‑1 (MQ=255)
               aGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACa                                                                                                                        >  1:196273/1‑140 (MQ=255)
               aGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACa                                                                                                                        >  1:505135/1‑140 (MQ=255)
                gCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTcg                                                                                                                             <  1:709040/134‑1 (MQ=255)
                gCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTcg                                                                                                                             >  2:709040/1‑134 (MQ=255)
                        aGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGc                                                                                                               <  2:505135/140‑1 (MQ=255)
                           gACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCctc                                                                                                            <  1:972901/140‑1 (MQ=255)
                                gCAAAAAGCGCAAACCGGCCAGCATCAGCGGGGGCATGTTATGAAGCCCCCCTTTGATGACCAAAAAATTTAGCCCCCATACGACCACTCCCAGTGGCGCCAACACCCCATCTTTTCGCGACATTCTGCCGCCTCTGAAt                                                                                                       <  1:422916/140‑1 (MQ=255)
                                           aaaCCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTg                                                                                            >  2:653992/1‑140 (MQ=255)
                                           aaaCCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCAACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTg                                                                                            >  1:912361/1‑140 (MQ=255)
                                            aaCCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCACATACGACCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGt                                                                                           <  1:685211/140‑1 (MQ=255)
                                               cGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAg                                                                                        <  2:421960/140‑1 (MQ=255)
                                                   cAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGCCCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAAt                                                                                    <  1:1108733/140‑1 (MQ=255)
                                                   cAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGCCCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAAt                                                                                    <  2:641585/140‑1 (MQ=255)
                                                            cGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGc                                                                           >  1:893907/1‑140 (MQ=255)
                                                               gggCATTTTATGAAGCCCCCCTTTGATGACCAAAAAATTTAGCCCCCATACGCCCACTCCCAGTAGCGCCACCCCCCCATCTTTTCGCGACTTTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGa                                                                        <  2:196152/139‑1 (MQ=255)
                                                                ggCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGaa                                                                       >  1:152854/1‑140 (MQ=255)
                                                                  cATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGCCCACTCCCAGTGGCCCAAACCCCCCATCTTTCCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAAtt                                                                     <  2:252136/140‑1 (MQ=255)
                                                                  cATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAAtt                                                                     <  2:196273/140‑1 (MQ=255)
                                                                     tttATGAAGCCCCACTTTGATGCCCAAAAAATTTAGCCCCCATACGACCACTCCCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTAc                                                                  <  1:618709/139‑1 (MQ=255)
                                                                       tATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACtt                                                                <  1:57173/140‑1 (MQ=255)
                                                                                ccACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAAc                                                       >  1:328429/1‑140 (MQ=255)
                                                                                 cACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACa                                                      >  1:251945/1‑140 (MQ=255)
                                                                                         tgaCCAAAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTc                                              <  1:302687/140‑1 (MQ=255)
                                                                                           cccACAAAATTTACCCCCCATACGACCACTCCCAGTAGCCCCAACCCCCCATTTTTTCGCGACTTTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGt                                            <  2:893907/139‑1 (MQ=255)
                                                                                                aaaaTTTAGCCCCCATACGACCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTaa                                                        <  1:814768/123‑1 (MQ=255)
                                                                                                aaaaTTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTaa                                                        >  2:814768/1‑123 (MQ=255)
                                                                                                      aaGCCCCAATACCCCAACCCCCAGAAGCCCCACCCCCCCATTTTTCCGCAATATTTTCCCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTCCTTTTCTTTAACAGTTGATCCCTTAGTCGCCGGt                                 <  2:364398/139‑1 (MQ=255)
                                                                                                             cATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTacgacg                          >  2:148527/1‑140 (MQ=255)
                                                                                                                   aCCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTa                    >  2:993644/1‑140 (MQ=255)
                                                                                                                    ccACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTaa                   >  1:761716/1‑140 (MQ=255)
                                                                                                                          ccAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCa             >  2:570662/1‑140 (MQ=255)
                                                                                                                            aGTAGCGCCACCCCCCCATTTTTTCGCGACTTTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCaaa           <  2:251945/140‑1 (MQ=255)
                                                                                                                              tAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTATTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATa         <  1:217756/140‑1 (MQ=255)
                                                                                                                              tAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATa         >  1:38623/1‑140 (MQ=255)
                                                                                                                                gcgcCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAg       >  1:1083263/1‑140 (MQ=255)
                                                                                                                                 cgcCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGt      >  1:457355/1‑140 (MQ=255)
                                                                                                                                  gcCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTc     >  2:766610/1‑140 (MQ=255)
                                                                                                                                   ccAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCg    <  1:570662/140‑1 (MQ=255)
                                                                                                                                     aaCACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCt  <  2:457355/140‑1 (MQ=255)
                                                                                                                                     aaCACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCt  >  1:218734/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                        
TGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCT  >  NC_000913/1620982‑1621254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑