Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,448,347	T→C	I32V (ATC→GTC)	prmB ←	50S ribosomal subunit protein L3 N(5)‑glutamine methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,448,347	0	T	C	100.0%	257.9 / NA	66	I32V (ATC→GTC)	prmB	50S ribosomal subunit protein L3 N(5)‑glutamine methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/29);  total (37/29)

AACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGCAATAATTTCGGCGGCTAGT  >  NC_000913/2448210‑2448482                                                                                                                                          |                                                                                                                                        aacaaTACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGacat                                                                                                                                       >  1:499052/1‑140 (MQ=255)       aCGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCcg                                                                                                                                 >  2:642420/1‑140 (MQ=255)         ggTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCcgcg                                                                                                                               >  1:781755/1‑140 (MQ=255)         ggTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCcgcg                                                                                                                               <  2:656674/140‑1 (MQ=255)           tgtTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGGAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTAGCGTGACCGTACCAGGCATTTGCCGCGCt                                                                                                                             >  2:1198199/1‑140 (MQ=255)           tgtTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCt                                                                                                                             >  2:1057849/1‑140 (MQ=255)           tgtTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCt                                                                                                                             >  2:107097/1‑140 (MQ=255)             tttttCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGa                                                                                                                           >  1:889002/1‑140 (MQ=255)               tttCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAg                                                                                                                         <  2:642178/140‑1 (MQ=255)                 tCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCg                                                                                                                       <  2:149275/140‑1 (MQ=255)                 tCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCg                                                                                                                       <  1:382262/140‑1 (MQ=255)                           tCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGTCCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGAc                                                                                                             <  1:244277/140‑1 (MQ=255)                           tCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGAc                                                                                                             <  1:1057849/140‑1 (MQ=255)                           tCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGAc                                                                                                             <  2:889002/140‑1 (MQ=255)                               aCGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACAGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAgc                                                                                                         <  2:354914/140‑1 (MQ=255)                                    cgGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAAc                                                                                                    >  2:408987/1‑140 (MQ=255)                                    cgGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAAc                                                                                                    <  1:770287/140‑1 (MQ=255)                                         cgcATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTc                                                                                               >  2:1040879/1‑140 (MQ=255)                                                   ccGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGtt                                                                                     >  2:126279/1‑140 (MQ=255)                                                   ccGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGtt                                                                                     >  2:800157/1‑140 (MQ=255)                                                    cGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAAc                                                                                                    <  1:397379/124‑1 (MQ=255)                                                    cGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAAc                                                                                                    >  2:397379/1‑124 (MQ=255)                                                         tatCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGc                                                                               >  2:766688/1‑140 (MQ=255)                                                           tCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTc                                                                             >  1:364701/1‑140 (MQ=255)                                                           tCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTc                                                                             >  1:857013/1‑140 (MQ=255)                                                              aGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCAtt                                                                          >  1:962247/1‑140 (MQ=255)                                                              aGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTATGCAGCTCAtt                                                                          >  2:551294/1‑140 (MQ=255)                                                                cGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTa                                                                        >  1:559905/1‑140 (MQ=255)                                                                   cAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAg                                                                                >  1:204694/1‑129 (MQ=255)                                                                   cAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAg                                                                                <  2:204694/129‑1 (MQ=255)                                                                   cAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTg                                                                     >  2:1134953/1‑140 (MQ=255)                                                                     ggTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCt                                                                   >  1:135745/1‑140 (MQ=255)                                                                              aaGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACg                                                          <  2:559905/140‑1 (MQ=255)                                                                                ggCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTa                                                                        <  1:1086051/124‑1 (MQ=255)                                                                                ggCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTa                                                                        >  2:1086051/1‑124 (MQ=255)                                                                                ggCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGaa                                                        >  1:529007/1‑140 (MQ=255)                                                                                ggCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGaa                                                        >  1:1096883/1‑140 (MQ=255)                                                                                     cacCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAAttt                                                   >  2:118795/1‑140 (MQ=255)                                                                                      acCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAAtttt                                                  <  1:118795/140‑1 (MQ=255)                                                                                        cAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTAt                                                <  2:755537/140‑1 (MQ=255)                                                                                          gCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATcc                                              <  1:752199/140‑1 (MQ=255)                                                                                           cTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCa                                             <  1:1013238/140‑1 (MQ=255)                                                                                           cTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCa                                             <  1:510680/140‑1 (MQ=255)                                                                                           cTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCa                                             >  1:1115498/1‑140 (MQ=255)                                                                                           cTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCa                                             <  1:841434/140‑1 (MQ=255)                                                                                             gTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACg                                           <  2:1056590/140‑1 (MQ=255)                                                                                             gTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACg                                           <  1:800157/140‑1 (MQ=255)                                                                                              tACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGt                                          >  1:88719/1‑140 (MQ=255)                                                                                               aCGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTa                                         >  2:1191647/1‑140 (MQ=255)                                                                                                     tCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTATtcctc                                   >  1:385849/1‑140 (MQ=255)                                                                                                     tCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTATtcctc                                   >  2:1064913/1‑140 (MQ=255)                                                                                                     tCATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCC‑‑‑‑TGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAgg                               >  1:1181095/1‑140 (MQ=255)                                                                                                      cATCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTATtcctcc                                  <  1:1191647/140‑1 (MQ=255)                                                                                                        tCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTa                                                 >  2:49808/1‑123 (MQ=255)                                                                                                        tCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTa                                                 <  1:49808/123‑1 (MQ=255)                                                                                                        tCCCACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAg                                >  2:862589/1‑140 (MQ=255)                                                                                                           cACGGGTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGc                             <  2:557589/140‑1 (MQ=255)                                                                                                                gTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGAt                        <  1:642420/140‑1 (MQ=255)                                                                                                                gTTATCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCATCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGAt                        <  1:852678/140‑1 (MQ=255)                                                                                                                   aTCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGc                     >  2:725987/1‑140 (MQ=255)                                                                                                                    tCGGTACCGTGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGCa                    <  1:725987/140‑1 (MQ=255)                                                                                                                             tGACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGCAATAATTTcg           >  2:1029476/1‑140 (MQ=255)                                                                                                                              gACCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGCAATAATTTcgg          <  2:88719/140‑1 (MQ=255)                                                                                                                               aCCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGCAATAATTTcggc         >  2:491616/1‑140 (MQ=255)                                                                                                                               aCCGTACCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGCAATAATTTcggc         >  1:493917/1‑140 (MQ=255)                                                                                                                                    aCCAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGCAATAATTTCGGCGGCTa    >  1:90528/1‑140 (MQ=255)                                                                                                                                     ccAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGCAATAATTTCGGCGGCTAg   >  1:1117426/1‑140 (MQ=255)                                                                                                                                      cAGACATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGCAATAATTTCGGCGGCTAGt  <  2:962247/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        AACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCCAGGGCATGATCGCAATAATTTCGGCGGCTAGT  >  NC_000913/2448210‑2448482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑