Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	889,923	A→G	intergenic (‑104/‑166)	ybjL ← / → ybjM	putative transport protein YbjL/putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	889,923	0	A	G	100.0%	251.0 / NA	63	intergenic (‑104/‑166)	ybjL/ybjM	putative transport protein YbjL/putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/39);  total (24/39)

CCATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAACGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTGGCGAAAA  >  NC_000913/889788‑890054                                                                                                                                        |                                                                                                                                    ccATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGAt                                                                                                                                 <  2:303932/140‑1 (MQ=255) ccATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGAt                                                                                                                                 <  1:320543/140‑1 (MQ=255)   aTTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATag                                                                                                                               >  1:256371/1‑140 (MQ=255)     ttAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATagta                                                                                                                             >  2:698563/1‑140 (MQ=255)      tAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATagtaa                                                                                                                            <  2:980561/140‑1 (MQ=255)         cAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATagtaaagt                                                                                                                         <  2:800863/140‑1 (MQ=255)             tCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACa                                                                                                                     <  1:111257/140‑1 (MQ=255)              cGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACaa                                                                                                                    <  1:219591/140‑1 (MQ=255)              cGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACaa                                                                                                                    <  1:482584/140‑1 (MQ=255)               ggCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAat                                                                                                                   >  2:698498/1‑140 (MQ=255)                 cGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAatat                                                                                                                 >  2:625686/1‑140 (MQ=255)                  gACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAatata                                                                                                                <  1:685436/140‑1 (MQ=255)                   aCGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAatatat                                                                                                               <  2:376546/140‑1 (MQ=255)                      tttATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATagtaaagt                                                                                                                         <  1:1170251/127‑1 (MQ=255)                      tttATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATagtaaagt                                                                                                                         >  2:1170251/1‑127 (MQ=255)                      tttATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATAtttt                                                                                                            >  1:775893/1‑140 (MQ=255)                                 ggAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAAt                                                                                                 <  1:409777/140‑1 (MQ=255)                                 ggAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAAt                                                                                                 <  1:482676/140‑1 (MQ=255)                                   aGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATca                                                                                               >  1:991901/1‑140 (MQ=255)                                     gCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTAAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATcaca                                                                                             <  2:32707/140‑1 (MQ=255)                                      cTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACAt                                                                                            >  2:822305/1‑140 (MQ=255)                                         aCTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAg                                                                                         >  1:506105/1‑140 (MQ=255)                                             cttGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTAt                                                                                     >  1:356173/1‑140 (MQ=255)                                              ttGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATc                                                                                    <  2:775893/140‑1 (MQ=255)                                               tGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCa                                                                                   <  1:547854/140‑1 (MQ=255)                                               tGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCa                                                                                   <  1:822305/140‑1 (MQ=255)                                               tGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCa                                                                                   >  2:890436/1‑140 (MQ=255)                                                 tttACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAAc                                                                                 <  2:694602/140‑1 (MQ=255)                                                  ttACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACg                                                                                <  1:1172450/140‑1 (MQ=255)                                                   tACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGTGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACgg                                                                               >  2:3068/1‑140 (MQ=255)                                                     cTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCt                                                                             >  1:881679/1‑140 (MQ=255)                                                                ttGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGc                                                                  >  2:635365/1‑140 (MQ=255)                                                                ttGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGc                                                                  >  1:686615/1‑140 (MQ=255)                                                                ttGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGc                                                                  >  2:650882/1‑140 (MQ=255)                                                                 tGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCg                                                                 <  1:1139657/140‑1 (MQ=255)                                                                  gaaagaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGt                                                                <  2:578366/140‑1 (MQ=255)                                                                  gaaagaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGt                                                                <  1:985823/140‑1 (MQ=255)                                                                  gaaagaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGt                                                                >  2:1055979/1‑140 (MQ=255)                                                                  gaaagaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGt                                                                >  2:93617/1‑140 (MQ=255)                                                                    aagaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTg                                                              <  1:1091723/140‑1 (MQ=255)                                                                    aagaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTg                                                              <  2:166041/140‑1 (MQ=255)                                                                    aagaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTg                                                              <  1:681289/140‑1 (MQ=255)                                                                     agaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTgg                                                             <  1:890436/140‑1 (MQ=255)                                                                      gaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGc                                                            <  2:935648/140‑1 (MQ=255)                                                                      gaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGc                                                            <  2:256371/140‑1 (MQ=255)                                                                      gaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGc                                                            <  1:698498/140‑1 (MQ=255)                                                                       aaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGcc                                                           <  2:881679/140‑1 (MQ=255)                                                                       aaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGcc                                                           <  2:986576/140‑1 (MQ=255)                                                                               aTTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGTGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAg                                                   <  1:3068/140‑1 (MQ=255)                                                                               aTTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAg                                                   >  2:575/1‑140 (MQ=255)                                                                                  tACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCAt                                                <  1:635365/140‑1 (MQ=255)                                                                                    cGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATgg                                              >  2:1149890/1‑140 (MQ=255)                                                                                      aTAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGaa                                            >  2:493543/1‑140 (MQ=255)                                                                                            ttttttACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGaa                                      <  1:553455/140‑1 (MQ=255)                                                                                              ttttACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGc                                    <  2:417218/140‑1 (MQ=255)                                                                                              ttttACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGc                                    <  2:110972/140‑1 (MQ=255)                                                                                                  aCCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCt                                <  1:1149890/140‑1 (MQ=255)                                                                                                      gAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTAt                            >  1:520444/1‑140 (MQ=255)                                                                                                            aGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACt                      <  2:356173/140‑1 (MQ=255)                                                                                                                 gcAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACg                 >  1:885776/1‑140 (MQ=255)                                                                                                                   aGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGcc               >  1:1109661/1‑140 (MQ=255)                                                                                                                      ttcattcaGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCcaca            <  2:686615/140‑1 (MQ=255)                                                                                                                                gcACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTGGCGaaaa  <  2:1109661/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    CCATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAACGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTGGCGAAAA  >  NC_000913/889788‑890054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑