Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,142,926	G→T	T481N (ACC→AAC)	rne ←	ribonuclease E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,142,926	0	G	T	100.0%	142.1 / NA	42	T481N (ACC→AAC)	rne	ribonuclease E
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/25);  total (17/25)

ACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGGTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGG  >  NC_000913/1142799‑1143060                                                                                                                                |                                                                                                                                       aCGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGAt                                                                                                                            >  2:706645/1‑140 (MQ=255)     ttACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGtt                                                                                                                        >  1:520736/1‑140 (MQ=255)        cGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTgg                                                                                                                     <  1:106908/140‑1 (MQ=255)        cGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTgg                                                                                                                     <  1:368142/140‑1 (MQ=255)               cGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAAtt                                                                                                              >  2:861428/1‑140 (MQ=255)               cGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAAtt                                                                                                              <  2:338146/140‑1 (MQ=255)                 aaCTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCTTCTGATCGTTTGGCACAATTac                                                                                                            <  2:859228/140‑1 (MQ=255)                         ttcAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAGGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGca                                                                                                    <  1:633821/140‑1 (MQ=255)                         ttcAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGca                                                                                                    <  2:1153632/140‑1 (MQ=255)                           cAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGcaca                                                                                                  <  1:183662/140‑1 (MQ=255)                              aCGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCg                                                                                               >  2:977255/1‑140 (MQ=255)                                    gcgcCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGa                                                                                         <  2:820769/140‑1 (MQ=255)                                          tCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGtt                                                                                   >  1:992688/1‑140 (MQ=255)                                               tcttcATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAAt                                                                              >  1:121785/1‑140 (MQ=255)                                                  tcATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGc                                                                           <  1:938148/140‑1 (MQ=255)                                                    aTGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCAt                                                                         >  2:815232/1‑140 (MQ=255)                                                       cAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCATCCTGACGAGTTTCAATGGCATTTa                                                                      <  2:187981/140‑1 (MQ=255)                                                          cTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCg                                                                   >  2:1075109/1‑140 (MQ=255)                                                          cTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCg                                                                   <  1:404525/140‑1 (MQ=255)                                                                cagcaTGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAAc                                                             <  2:470142/140‑1 (MQ=255)                                                                      gTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAAc                                                             <  2:550808/134‑1 (MQ=255)                                                                      gTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAAc                                                             >  1:550808/1‑134 (MQ=255)                                                                        aGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTttca                                                     <  2:1075350/140‑1 (MQ=255)                                                                        aGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTttca                                                     >  2:371214/1‑140 (MQ=255)                                                                             aaGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTcag                                                <  2:74377/140‑1 (MQ=255)                                                                             aaGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTcag                                                <  2:121785/140‑1 (MQ=255)                                                                                    gggTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTaa                                         >  1:705456/1‑140 (MQ=255)                                                                                    gggTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTaa                                         >  1:589314/1‑140 (MQ=255)                                                                                         tcttcCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGc                                    >  2:959996/1‑140 (MQ=255)                                                                                           ttcCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGa                                  >  1:978032/1‑140 (MQ=255)                                                                                             ccccTTTACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGAtt                                <  2:305442/140‑1 (MQ=255)                                                                                                  ttACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGcac                           <  1:371214/140‑1 (MQ=255)                                                                                                  ttACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGcac                           <  2:810297/140‑1 (MQ=255)                                                                                                  ttACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGcac                           <  2:854809/140‑1 (MQ=255)                                                                                                  ttACGCACGCGCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGcac                           <  1:1145930/140‑1 (MQ=255)                                                                                                         cgcgCAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACa                    <  1:977255/140‑1 (MQ=255)                                                                                                           cgcAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAAt                  >  2:401198/1‑140 (MQ=255)                                                                                                           cgcAGCACGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAAt                  >  1:830151/1‑140 (MQ=255)                                                                                                                 aCGTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTg            <  1:815232/140‑1 (MQ=255)                                                                                                                   gTGGTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGaa          <  2:520736/140‑1 (MQ=255)                                                                                                                      gTAGTGCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACtt       >  2:1188489/1‑140 (MQ=255)                                                                                                                           gCGGGTTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTgg  <  1:1075109/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                       ACGCTTACGTTCAGCGAACTCTTCTTCAGACGGCAGCGCCATCGCTTCTTCATGCAGCTTCGGCAGCATGTAGCTTAAGGTTGGGGTTTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGGTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGG  >  NC_000913/1142799‑1143060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑