Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,393,460	C→A	C203F (TGC→TTC)	queG ←	epoxyqueuosine reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,393,460	0	C	A	100.0%	286.2 / NA	81	C203F (TGC→TTC)	queG	epoxyqueuosine reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (41/40);  total (41/40)

AGTCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTAC  >  NC_000913/4393323‑4393595                                                                                                                                          |                                                                                                                                        aGTCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGaaa                                                                                                                                       >  1:1426141/1‑140 (MQ=255) aGTCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGaaa                                                                                                                                       >  1:630295/1‑140 (MQ=255) aGTCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGaaa                                                                                                                                       >  1:389044/1‑140 (MQ=255) aGTCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGaaa                                                                                                                                       >  1:1230296/1‑140 (MQ=255) aGTCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGaaa                                                                                                                                       >  2:1411476/1‑140 (MQ=255)   tCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAAt                                                                                                                                     <  2:377189/140‑1 (MQ=255)    cATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATc                                                                                                                                    <  1:1153082/140‑1 (MQ=255)     aTCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCg                                                                                                                                   <  2:389044/140‑1 (MQ=255)      tCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGt                                                                                                                                  <  1:1142222/140‑1 (MQ=255)         cAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCAt                                                                                                                               >  1:868350/1‑140 (MQ=255)          aaCCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATa                                                                                                                              <  1:803700/140‑1 (MQ=255)          aaCCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATa                                                                                                                              <  1:898892/140‑1 (MQ=255)             cGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAg                                                                                                                           >  1:446814/1‑140 (MQ=255)               tAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGc                                                                                                                         >  2:622358/1‑140 (MQ=255)                      ggTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCAt                                                                                                                  >  1:984430/1‑140 (MQ=255)                          tCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGc                                                                                                              >  1:986766/1‑140 (MQ=255)                             cATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGc                                                                                                           <  2:630295/140‑1 (MQ=255)                               ttAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCAt                                                                                                         <  2:11952/140‑1 (MQ=255)                                  aCGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCAtcct                                                                                                      >  2:1372182/1‑140 (MQ=255)                                      tCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCAtccttcct                                                                                                  >  1:1382896/1‑140 (MQ=255)                                      tCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCAtccttcct                                                                                                  >  2:1443639/1‑140 (MQ=255)                                        gCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCg                                                                                                <  2:178066/140‑1 (MQ=255)                                         cAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGa                                                                                               <  2:853132/140‑1 (MQ=255)                                         cAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGa                                                                                               <  2:384672/140‑1 (MQ=255)                                         cAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGa                                                                                               <  1:730574/140‑1 (MQ=255)                                               ttCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGtt                                                                                         <  1:158421/140‑1 (MQ=255)                                                 ccGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGa                                                                                       >  1:320256/1‑140 (MQ=255)                                                  cGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGAt                                                                                      <  1:1072167/140‑1 (MQ=255)                                                    ggATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATcc                                                                                    >  2:1182505/1‑140 (MQ=255)                                                       tCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACg                                                                                 >  1:1016512/1‑140 (MQ=255)                                                       tCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACg                                                                                 >  1:803963/1‑140 (MQ=255)                                                        cGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACgg                                                                                <  1:632820/140‑1 (MQ=255)                                                         gCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACggg                                                                               >  1:399209/1‑140 (MQ=255)                                                         gCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACggg                                                                               >  1:827968/1‑140 (MQ=255)                                                         gCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACggg                                                                               <  2:804349/140‑1 (MQ=255)                                                            ccTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAg                                                                            <  2:803963/140‑1 (MQ=255)                                                            ccTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAg                                                                            <  2:1016512/140‑1 (MQ=255)                                                              ttCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCg                                                                          <  2:1382896/140‑1 (MQ=255)                                                                    ttCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAatat                                                                    >  2:11426/1‑140 (MQ=255)                                                                        aTGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGAc                                                                >  1:1426073/1‑140 (MQ=255)                                                                                   agagATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGc                                                     >  1:1240399/1‑140 (MQ=255)                                                                                     agaTACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCt                                                   <  1:11426/140‑1 (MQ=255)                                                                                     agaTACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCt                                                   <  2:399209/140‑1 (MQ=255)                                                                                      gaTACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTa                                                  <  1:655701/140‑1 (MQ=255)                                                                                          cAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTaaaaa                                              >  1:342418/1‑140 (MQ=255)                                                                                          cAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTaaaaa                                              <  1:1411476/140‑1 (MQ=255)                                                                                          cAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTaaaaa                                              >  1:1266330/1‑140 (MQ=255)                                                                                          cAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTaaaaa                                              >  2:761660/1‑140 (MQ=255)                                                                                          cAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTACCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTaaaaa                                              >  1:8346/1‑140 (MQ=255)                                                                                          cAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACACATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTaaaaa                                              >  2:10428/1‑140 (MQ=255)                                                                                             cggcgAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAaagaa                                           >  1:344913/1‑140 (MQ=255)                                                                                             cggcgAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAaagaa                                           >  2:596331/1‑140 (MQ=255)                                                                                              ggcgAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAaag                                             >  1:468927/1‑137 (MQ=255)                                                                                              ggcgAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAaag                                             <  2:468927/137‑1 (MQ=255)                                                                                               gcgAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAagaaga                                         <  2:984430/140‑1 (MQ=255)                                                                                               gcgAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAagaaga                                         <  2:1426073/140‑1 (MQ=255)                                                                                               gcgAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAagaaga                                         >  1:1258107/1‑140 (MQ=255)                                                                                                cgAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAaaga                                            <  2:854730/136‑1 (MQ=255)                                                                                                cgAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAaaga                                            >  1:854730/1‑136 (MQ=255)                                                                                                  aGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAgaacg                                      >  2:447715/1‑140 (MQ=255)                                                                                                  aGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAgaacg                                      >  2:828739/1‑140 (MQ=255)                                                                                                  aGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAgaacg                                      >  1:1020150/1‑140 (MQ=255)                                                                                                  aGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAgaacg                                      >  1:144882/1‑140 (MQ=255)                                                                                                       cGACGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCg                                 >  1:700512/1‑140 (MQ=255)                                                                                                         aCGGTATATGGCTCGACGATGACACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGc                               <  1:817792/140‑1 (MQ=255)                                                                                                             tataTGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTcg                           <  1:1301608/140‑1 (MQ=255)                                                                                                             tataTGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTcg                           >  2:1301608/1‑140 (MQ=255)                                                                                                             tataTGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTcg                           <  1:685185/140‑1 (MQ=255)                                                                                                                atGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTCGCGa                        <  1:622358/140‑1 (MQ=255)                                                                                                                  ggCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTCGCGAtt                      <  2:986766/140‑1 (MQ=255)                                                                                                                  ggCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTCGCGAtt                      <  2:1443410/140‑1 (MQ=255)                                                                                                                  ggCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTCGCGAtt                      <  2:1096221/140‑1 (MQ=255)                                                                                                                    cTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTCGCGATTGa                    >  1:213067/1‑140 (MQ=255)                                                                                                                     tCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTCGCGATTGAg                   >  1:854567/1‑140 (MQ=255)                                                                                                                       gacgaTGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTc                            <  1:77189/129‑1 (MQ=255)                                                                                                                       gacgaTGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTc                            >  2:77189/1‑129 (MQ=255)                                                                                                                       gacgaTGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTCGCGATTGAGGa                 <  2:649482/140‑1 (MQ=255)                                                                                                                       gacgaTGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTCGCGATTGAGGa                 >  1:460060/1‑140 (MQ=255)                                                                                                                       gacgaTGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTCGCGATTGAGGa                 >  1:338743/1‑140 (MQ=255)                                                                                                                       gacgaTGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTCGCGATTGAGGa                 <  2:948015/140‑1 (MQ=255)                                                                                                                       gacgaTGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTCGCGATTGAGGa                 >  1:1050541/1‑140 (MQ=255)                                                                                                                        acgaTGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTCGCGATTGAGGAt                <  2:854567/140‑1 (MQ=255)                                                                                                                         cgaTGGCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTCGCGATTGAGGATa               >  2:512081/1‑140 (MQ=255)                                                                                                                             ggCACCGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTCGCGATTGAGGATAagtg           >  2:27080/1‑140 (MQ=255)                                                                                                                                 ccGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTCGCGATTGAGGATAagtgagtg       <  1:403443/140‑1 (MQ=255)                                                                                                                                 ccGGTCGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTCGCGATTGAGGATAagtgagtg       <  2:338743/140‑1 (MQ=255)                                                                                                                                      cGGGAAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTAc  <  2:1020150/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        AGTCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCACGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTAC  >  NC_000913/4393323‑4393595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑