Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,258,441	C→G	A25P (GCC→CCC)	pth ←	peptidyl‑tRNA hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,258,441	0	C	G	100.0%	156.5 / NA	41	A25P (GCC→CCC)	pth	peptidyl‑tRNA hydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (24/17);  total (24/17)

ATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGCACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAGGCCGACAATCAATTTAATCGTCACGTTTTTTTGTCCTGAGTGTGTACATAACTGGCGCGTAGTTTACTGGTTGCGGCCCCGCT  >  NC_000913/1258307‑1258572                                                                                                                                       |                                                                                                                                    aTTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGCGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAg                                                                                                                                >  2:222385/1‑140 (MQ=255)      tAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTa                                                                                                                           >  1:443577/1‑140 (MQ=255)             aGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTcgcg                                                                                                                    >  1:176098/1‑140 (MQ=255)              gTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGt                                                                                                                   >  1:436802/1‑140 (MQ=255)               tCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGtt                                                                                                                  >  2:570045/1‑140 (MQ=255)                  ggACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTgcg                                                                                                               <  2:144835/140‑1 (MQ=255)                  ggACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTgcg                                                                                                               <  1:546722/140‑1 (MQ=255)                    aCTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCATCTGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTgcggc                                                                                                             <  2:132257/140‑1 (MQ=255)                    aCTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTgcggc                                                                                                             >  2:20218/1‑140 (MQ=255)                    aCTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTgcggc                                                                                                             >  2:170347/1‑140 (MQ=255)                               gACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCAc                                                                                                  <  1:145302/140‑1 (MQ=255)                               gACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCAc                                                                                                  >  2:219598/1‑140 (MQ=255)                               gACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCAc                                                                                                  <  2:139958/140‑1 (MQ=255)                                            ccAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAg                                                                                     >  2:404428/1‑140 (MQ=255)                                             cAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCggg                                                                                    >  1:479390/1‑140 (MQ=255)                                                   tGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAGGCCGAca                                                                              <  2:176098/140‑1 (MQ=255)                                                    gACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAGGCCGAcaa                                                                             >  1:398449/1‑140 (MQ=255)                                                     aCTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAGGCCGAcaat                                                                            >  1:719318/1‑140 (MQ=255)                                                          cgAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAGGCCGACAATCAAtt                                                                       >  1:57218/1‑140 (MQ=255)                                                                        aGAATTTAGACTCTTCGCGCAGCGGAGCGCGCAAACTCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGCTCGCCAGCCCGACAATCAATTTAATCGTCACGttt                                                         >  2:503814/1‑140 (MQ=255)                                                                         gAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAGGCCGACAATCAATTTAATCGTCACGtttt                                                        >  1:97449/1‑140 (MQ=255)                                                                         gAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAGGCCGACAATCAATTTAATCGTCACGtttt                                                        <  1:570045/140‑1 (MQ=255)                                                                         gAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAGGCCGACAATCAATTTAATCGTCACGtttt                                                        >  2:549980/1‑140 (MQ=255)                                                                           aTTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAGGCCGACAATCAATTTAATCGTCACGtttttt                                                      <  2:586346/140‑1 (MQ=255)                                                                            tttAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAGGCCGACAATCAATTTAATCGTCACGttttgtt                                                     >  2:82137/1‑140 (MQ=255)                                                                                 ccTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAGGCCGACAATCAATTTAATCGTCACGTTTTTTTGTCCt                                                <  1:20218/140‑1 (MQ=255)                                                                                 ccTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAGGCCGACAATCAATTTAATCGTCACGTTTTTTTGTCCt                                                <  2:614402/140‑1 (MQ=255)                                                                                  ctctTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAGGCCGACAATCAATTTAATCGTCACGTTTTTTTGTCCTg                                               <  2:57218/140‑1 (MQ=255)                                                                                    ctTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAGGCCGACAATCAATTTAATCGTCACGTTTTTTTGTCCTGAg                                             >  1:356246/1‑140 (MQ=255)                                                                                     ttCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAGGCCGACAATCAATTTAATCGTCACGTTTTTTTGTCCTGAgt                                            <  2:479390/140‑1 (MQ=255)                                                                                     ttCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAGGCCGACAATCAATTTAATCGTCACGTTTTTTTGTCCTGAgt                                            <  1:664717/140‑1 (MQ=255)                                                                                                 aGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAGGCCGACAATCAATTTAATCGTCACGTTTTTTTGTCCTGAGTGTGTActgtctc                                >  2:605404/1‑134 (MQ=255)                                                                                                    gcgcAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAGGCCGACAATCAATTTAATCGTCACGTTTTTTTGTCCTGAGTGTGTACATAACTGgc                             <  2:719318/140‑1 (MQ=255)                                                                                                    gcgcAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAGGCCGACAATCAATTTAATCGTCACGTTTTTTTGTCCTGAGTGTGTACATAACTGgc                             >  1:452388/1‑140 (MQ=255)                                                                                                      gcAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCATCACCGGGGTTCGCCAGGCCGACAATCAATTTAATCGTCACGTTTTTTTGTCCTGAGTGTGTACATAACTGgcgc                           <  2:683761/140‑1 (MQ=255)                                                                                                       cAAACGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAGGCCGACAATCAATTTAATCGTCACGTTTTTTTGTCCTGAGTGTGTACATAACTGGcgcg                          <  1:170347/140‑1 (MQ=255)                                                                                                        aaaCGCTCTGCCAGTAAGTCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAGGCCGACAATCAATTTAATCGTCACGTTTTTTTGTCCTGAGTGTGTACATAACTGGCGCGt                         >  2:340288/1‑140 (MQ=255)                                                                                                                          tCAACGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAGGCCGACAATCAATTTAATCGTCACGTTTTTTTGTCCTGAGTGTGTACATAACTGGCGCGTAGTTTACTGGTTGCGGcc       >  1:23512/1‑140 (MQ=255)                                                                                                                            aaCGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAGGCCGACAATCAATTTAATCGTCACGTTTTTTTGTCCTGAGTGTGTACATAACTGGCGCGTAGTTTACTGGTTGCGGcccc     >  2:612158/1‑140 (MQ=255)                                                                                                                            aaCGAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAGGCCGACAATCAATTTAATCGTCACGTTTTTTTGTCCTGAGTGTGTACATAACTGGCGCGTAGTTTACTGGTTGCGGcccc     >  1:571790/1‑140 (MQ=255)                                                                                                                               gAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAGGCCGACAATCAATTTAATCGTCACGTTTTTTTGTCCTGAGTGTGTACATAACTGGCGCGTAGTTTACTGGTTGCGGCCCCGCt  <  2:660052/140‑1 (MQ=255)                                                                                                                               gAACCAGGGACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAGGCCGACAATCAATTTAATCGTCACGTTTTTTTGTCCTGAGTGTGTACATAACTGGCGCGTAGTTTACTGGTTGCGGCCCCGCt  >  2:146679/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                    ATTCATAAATGTAGTCGGGACTAACAGGCGGACATCTTCGCCTCCAAGAGTGACTCGCGAAGTATAACCAAAGAATTTAGCCTCTTCGCGCAGCGGAGCGCGCAAACGCTCTGCCAGTAAGTCAACGAACCAGGCACCAGCATTATGTCGCGTTGCGGCGTATTCAGCACCGGGGTTCGCCAGGCCGACAATCAATTTAATCGTCACGTTTTTTTGTCCTGAGTGTGTACATAACTGGCGCGTAGTTTACTGGTTGCGGCCCCGCT  >  NC_000913/1258307‑1258572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑