Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	445,558	0	A	C	22.4%	92.8 / 20.7	49	V370G (GTA→GGA)	yajR	putative major facilitator superfamily transport protein YajR
Reads supporting (aligned to +/- strand):  ref base A (27/11);  new base C (0/11);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 2.42e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGA  >  NC_000913/445420‑445694                                                                                                                                           |                                                                                                                                         aGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATAc                                                                                                                                         >  1:889054/1‑140 (MQ=255)      aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTTATGGTACTGGCGACTGTCAGCCCCACTGCGGCCCGCATTGCGCCCGCGAGGAATAcccccc                                                                                                                                    >  2:893852/1‑139 (MQ=255)      aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                    <  1:381607/140‑1 (MQ=255)      aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                    <  2:889054/140‑1 (MQ=255)      aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGACTTCAATGCGCAAACTGCTGACATACGGCGGTTTTTTTATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCCGCGAGAAAAAcccccc                                                                                                                                    >  2:775663/1‑139 (MQ=255)                aaCGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCaaa                                                                                                                          <  1:775663/140‑1 (MQ=255)                   gCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACAt                                                                                                                       <  1:1333897/140‑1 (MQ=255)                   gCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACAt                                                                                                                       <  1:893852/140‑1 (MQ=255)                   gCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACAt                                                                                                                       <  2:1045647/140‑1 (MQ=255)                   gCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACAt                                                                                                                       <  1:589338/140‑1 (MQ=255)                   gCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCCTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACAt                                                                                                                       <  1:149988/140‑1 (MQ=255)                    ccTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGGACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCCGCGGGAAATAACCCCCGACCGTCCAACAta                                                                                                                      >  2:279738/1‑139 (MQ=255)                      tCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCCGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGcc                                                                                                                    >  1:404351/1‑140 (MQ=255)                       cGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCg                                                                                                                   <  2:1323385/140‑1 (MQ=255)                                  aTGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAgcc                                                                                                        <  1:588401/140‑1 (MQ=255)                                   tGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTTATGGTACTGGCGACTGTCAGCCACACCGCGGCCAGCATTGCGCCAGCGAGAAAAACCCCCCGACCGTCAAACATACCGCTAATCCAgccc                                                                                                       >  2:1048836/1‑139 (MQ=255)                                     ttCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAgccgcc                                                                                                     <  1:1123366/140‑1 (MQ=255)                                        gCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGcccca                                                                                                  >  1:915504/1‑138 (MQ=255)                                          cGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTTTTTTATGGGACTGGCGACTGTCAGCCACACCGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCCGACCGTCAAACATGCCGTTAATCCAGCCGCCCCGcc                                                                                                >  2:1317126/1‑139 (MQ=255)                                               tttCAATGCGCAAACTGCTGACATACGGCGGTTCTTTTATGGTACTGGCGACTGTCAGCCACACTGCGGGCCGCCATTCGCCAGCGAGAAATACCCCCCGACCGGCAAACATGCCCGTTATCCAACCGCCCAGGGAAccc                                                                                           >  2:428163/1‑139 (MQ=255)                                                         cAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAAAAACCCCCGACCGTCAAACATGCCGTTAATCCCGCCCCCCAGCGAAACCCCCATccccc                                                                                 >  2:748706/1‑139 (MQ=255)                                                              tgctgACATCGGGCGTTTTTTTCATGGTACTGGCGCCTGTCAGCCACCCGGCGGCCAGCATTGCGCCGGCGAAAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgccacgcca                                                                            <  2:856762/140‑1 (MQ=255)                                                                        cggcggTTCTTTCATGGGACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCCGCGAGAAATACCCCCCGACCCTCAAACATGCCGTTAATCCAGCCGCCCCGCGAACCCCCAATCGCCACGCCAAGAAAatggc                                                                  >  2:905373/1‑140 (MQ=255)                                                                               tCTTTCATGGTACTGGCGACTGTCAGCCACACCGCGGCCAGCATTTCGCCAGCGAGAAAAACCCCCCGACCGTCAAACATGCCCTTAATCCAGCCCCCCAGCGAACCGCCAATCCCCACGCCAAGAAACTGGCtgggggg                                                           >  2:454496/1‑139 (MQ=255)                                                                                         tACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAccc                                                 >  2:1002434/1‑140 (MQ=255)                                                                                         tACGGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAccc                                                 <  1:179334/140‑1 (MQ=255)                                                                                          aCTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCa                                                >  1:1202183/1‑140 (MQ=255)                                                                                              gCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGc                                            >  2:1257049/1‑140 (MQ=255)                                                                                                   tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                        <  1:1426936/139‑1 (MQ=255)                                                                                                   tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                        <  1:975469/139‑1 (MQ=255)                                                                                                   tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTaaa                                                      <  1:1239583/125‑1 (MQ=255)                                                                                                   tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTaaa                                                      >  2:1239583/1‑125 (MQ=255)                                                                                                   tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                        >  2:975469/1‑139 (MQ=255)                                                                                                   tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                        >  2:1426936/1‑139 (MQ=255)                                                                                                   tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                       >  2:1381000/1‑140 (MQ=255)                                                                                                   tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                       >  2:880762/1‑140 (MQ=255)                                                                                                   tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                       >  2:1170101/1‑140 (MQ=255)                                                                                                     tCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCt                                     >  2:984899/1‑140 (MQ=255)                                                                                                       aGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCttt                                   >  1:681815/1‑140 (MQ=255)                                                                                                        gCCACACTGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGACCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTg                                  <  1:840395/140‑1 (MQ=255)                                                                                                            cccTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAAc                              <  1:1109688/138‑1 (MQ=255)                                                                                                               tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                           >  1:1261730/1‑140 (MQ=255)                                                                                                               tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                           >  1:168406/1‑140 (MQ=255)                                                                                                               tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                           >  1:419153/1‑140 (MQ=255)                                                                                                                 ccgCCAGCATTGCGCCAGCGAGAAATACCCCCCGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctgct                         >  2:255585/3‑140 (MQ=255)                                                                                                                 cGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctgct                         >  1:1053842/1‑140 (MQ=255)                                                                                                                 cGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctgct                         >  2:1427161/1‑140 (MQ=255)                                                                                                                 cGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGGAACctgct                         >  1:1201767/1‑140 (MQ=255)                                                                                                                   gCCAGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                       <  1:392759/140‑1 (MQ=255)                                                                                                                   gCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                       >  2:1096438/1‑140 (MQ=255)                                                                                                                   gCCAGCATTGCGCAAGCGAAAAATCCCCCCTGACCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                       <  2:1246951/140‑1 (MQ=255)                                                                                                                    ccAGCATGGCGCCAGCGAGAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGc                      <  2:1220269/140‑1 (MQ=255)                                                                                                                    ccAGCATGGCGCCAGCGAAAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGc                      <  2:404351/140‑1 (MQ=255)                                                                                                                      gccATGGCGCCAGCGAAAAATCCCCCCTGCCGGGCAAACATGCGGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                    <  2:1202183/138‑1 (MQ=255)                                                                                                                       gCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAc                   <  1:880762/140‑1 (MQ=255)                                                                                                                        cATTGCGCCAGCGAAAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCACCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAct                  <  2:878976/140‑1 (MQ=255)                                                                                                                        cATTGCCCCAGCGAAAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAct                  <  2:419153/140‑1 (MQ=255)                                                                                                                         aTTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActc                 >  1:1220269/1‑140 (MQ=255)                                                                                                                           tGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCtt               >  2:950105/1‑140 (MQ=255)                                                                                                                            gcgcCAGCGAGAAATCCCCCCTGACCGTCAACCATGCCGTTAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt              <  1:279738/140‑1 (MQ=255)                                                                                                                            gcgcCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt              <  1:1257049/140‑1 (MQ=255)                                                                                                                             cgcCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAGTCTTTa             >  2:1378967/1‑140 (MQ=255)                                                                                                                             cgcCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTa             >  1:316652/1‑140 (MQ=255)                                                                                                                               caaGCGAAAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAACGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACt           <  2:915504/138‑1 (MQ=255)                                                                                                                                cAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTg          <  1:984899/140‑1 (MQ=255)                                                                                                                                  gCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt        >  2:528672/1‑140 (MQ=255)                                                                                                                                  gCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt        >  1:66177/1‑140 (MQ=255)                                                                                                                                  gCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt        >  2:974538/1‑140 (MQ=255)                                                                                                                                        aaTCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGa  <  1:985304/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                         AGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGA  >  NC_000913/445420‑445694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑