Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	753,114	0	G	T	24.4%	218.8 / 17.1	82	intergenic (‑319/+71)	ybgD/gltA	putative fimbrial protein YbgD/citrate synthase
Reads supporting (aligned to +/- strand):  ref base G (46/16);  new base T (0/20);  total (46/36)
Fisher's exact test for biased strand distribution p-value = 1.18e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGA  >  NC_000913/752977‑753245                                                                                                                                          |                                                                                                                                    aTTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGtt                                                                                                                                   >  1:83243/1‑140 (MQ=255) aTTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGtt                                                                                                                                   <  2:1356917/140‑1 (MQ=255) aTTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGtt                                                                                                                                   <  1:1376423/140‑1 (MQ=255) aTTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTC‑TTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGttt                                                                                                                                  <  2:373534/140‑1 (MQ=255)      cATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGc                                                                                                                              <  2:795539/140‑1 (MQ=255)             ttATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATaaa                                                                                                                       >  2:283232/1‑140 (MQ=255)             ttATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTT‑TGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATaaaa                                                                                                                      >  1:535863/1‑140 (MQ=255)               ataAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATaaaaa                                                                                                                     >  1:596580/1‑140 (MQ=255)               ataAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATaaaaa                                                                                                                     >  2:1166412/1‑140 (MQ=255)               ataAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATaaaaa                                                                                                                     >  2:620676/1‑140 (MQ=255)                 aaaTGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATc                                                                                                                   >  2:302188/1‑140 (MQ=255)                    tGCGACTACCATGAATTTTTTAATTAAAAGTTTTGGTTTGCTGATATTTTGGGCTGTTTTTTTTTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACATTTTTTTGTTTTTAGGAATAAAAAAATCacc                                                                                                                <  2:1266389/140‑3 (MQ=255)                     gtgACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAcc                                                                                                               >  1:993666/3‑140 (MQ=255)                     gCGACTACCATGAATTTTTTAATTGAAAGTTTTGGGTTGCTGATAATTTGAGCTTTTTTTTTCTTTTTAAATATCTATATAGGTCTTTTAATGGATTTTTTTTTTACAATTTTTTGTGTTTAGGCATAAAAAAATAAAcc                                                                                                               <  2:433140/140‑1 (MQ=255)                     gCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAcc                                                                                                               >  1:1181261/1‑140 (MQ=255)                     gCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAcc                                                                                                               >  1:907430/1‑140 (MQ=255)                      cGACTACCATGAATTTTTTAATTAAAAGTATTGGGTTGCTGATAATTTGAGCTTTTCTATTTTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTTTTTAGGCATATAAAAATCAcccc                                                                                                              <  2:808261/140‑1 (MQ=255)                      cGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAccc                                                                                                              >  1:190588/1‑140 (MQ=255)                      cGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAccc                                                                                                              >  2:367776/1‑140 (MQ=255)                       gACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCg                                                                                                             >  1:619076/1‑140 (MQ=255)                        aCTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGc                                                                                                            >  1:1109859/1‑140 (MQ=255)                         cTACCATGAATTTTTTAATTGAAAGTTTTGGGTGGCTGATAATTTGAGCTTTTTTTTTTTTTTTAAATATCTATATAGGTCTGTTAAGGGATTTTATTTTTACATTTTTTTTTGTTTAGGCATATAAAAATCAACCCGcc                                                                                                           <  2:887802/140‑1 (MQ=255)                             cATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCatat                                                                                                       >  1:433140/1‑140 (MQ=255)                               tGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGa                                                                                                     >  1:548962/1‑140 (MQ=255)                                      tttAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAA‑‑TTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGt                                                                                            >  2:667243/1‑140 (MQ=255)                                       ttAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCggg                                                                                             >  1:1115381/1‑140 (MQ=255)                                            tGAAAGTATTGGGTTGTTGATATTTTGAGCTTTTTTATTTTTTTTAAATATTTATATAGTTCTTTTAATGGTTTTTTTTTTTACATTTTTTTGTTTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTaaa                                                                                        <  2:1115381/140‑1 (MQ=255)                                              aaaGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAat                                                                                      >  1:552868/1‑140 (MQ=255)                                                        tttgttaaTAATTTGAGCTTTTTTTTTCTTTTTAAATATTTATATAGGTCTTTTAATGGATTTTATTTTTAAAATTTTTTGTTTTTAGGAATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACt                                                                            <  2:52934/133‑1 (MQ=255)                                                        tttGCTAATATTTTGAGTTTTTTTATTTTTTTTAAATATTTATATAGGTTTGTTAATGGTTTTTTTTTTTAAAATTTTTTGTGTTTAGGCATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACt                                                                            <  2:907430/139‑1 (MQ=255)                                                            ttaatatTTTGAGTTTTTCTTTTTTTTTAAAATATTTATATAGTTTTTTTAATGTTTTTTTTTTTTACATTTTTTTGTGTTTAGGAATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGc                                                                        <  2:619076/137‑1 (MQ=255)                                                            cTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTacc                                                                        >  2:1198028/1‑138 (MQ=255)                                                            cTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGc                                                                        >  1:1059533/1‑140 (MQ=255)                                                            cTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGc                                                                        >  2:1337763/1‑140 (MQ=255)                                                            cTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGc                                                                        >  1:1111209/1‑140 (MQ=255)                                                            cTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGc                                                                        >  1:75851/1‑140 (MQ=255)                                                              aatatTTTGAGTTTTTTTTTTTTTTTTAAATATCTATATAGGTTTGTTAATGGTTTTTTTTTTTACATTTTTTTGTTTTTAGGAATAAAAAAACCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCaa                                                                      <  2:777096/139‑1 (MQ=255)                                                               aTAATTTGAGCTTTTTTATTTTTTTTAAATATTTATATAGTTTTTTTAATGATTTTTTTTTTTAAATTTTTTTGTTTTTAGGAATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAAt                                                                     <  2:83243/140‑1 (MQ=255)                                                                tAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATc                                                                    <  1:283232/140‑1 (MQ=255)                                                                   tttGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAAc                                                                 >  1:1346127/1‑140 (MQ=255)                                                                      gAGCTTTTCTTTTTTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACATTTTTTTGTTTTTAGACATAAAAAAACCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCAt                                                              <  2:134064/140‑1 (MQ=255)                                                                       aGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCAtt                                                             >  1:645673/1‑140 (MQ=255)                                                                        gCTTTTTTATTTTTTTAAAATATTTATATAGGTTTGTTAATGGATTTTATTTTTAAATTTTTTTGTTTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTa                                                            <  1:209362/140‑1 (MQ=255)                                                                            ttCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGc                                                        >  1:156287/1‑140 (MQ=255)                                                                                 ttttttttAAATATTTATATAGGTTTTTTAATGGTTTTTTTTTTTACATTTTTTTGTTTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGat                                                   <  2:75851/137‑1 (MQ=255)                                                                                  tttttttAAATATTTATATAGGTCTGTTAATGGTTTTTATTTTTAAATTTTTTTGTGTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGata                                                  <  2:1424773/138‑1 (MQ=255)                                                                                   ttttttAAATATCTATATAGGTCTTTTAATGGATTTTTTTTTTAAATTTTTTTGTGTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGatat                                                 <  1:737950/139‑1 (MQ=255)                                                                                      tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                              >  1:1002842/1‑140 (MQ=255)                                                                                      tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                              >  1:8263/1‑140 (MQ=255)                                                                                      tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                              >  1:409814/1‑140 (MQ=255)                                                                                        tAAATATCTATATAGGTTTTTTAATGGTTTTTTTTTTTACATTTTTTTGTTTTTAGGAATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCtt                                            <  2:1367052/140‑1 (MQ=255)                                                                                                    taGGTCTGTTAATGGATTTTATTTTTACATTTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGt                                <  2:536081/140‑1 (MQ=255)                                                                                                       gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGtttt                             >  1:741905/1‑140 (MQ=255)                                                                                                       gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGtttt                             >  2:754371/1‑140 (MQ=255)                                                                                                       gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGtttt                             >  1:853779/1‑140 (MQ=255)                                                                                                       gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGtttt                             >  2:1366204/1‑140 (MQ=255)                                                                                                       gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGtttt                             >  1:656015/1‑140 (MQ=255)                                                                                                       gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGGTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGtttt                             >  1:589952/1‑140 (MQ=255)                                                                                                         tttttAATGTTTTTTATTTTTAAATTTTTTTGTTTTTAGCAATAAAAAAAACAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTc                           <  2:1120758/137‑1 (MQ=255)                                                                                                         ttGTTAATGGATTTTTTTTTTACAATTTTTTGTTTTTAGGCATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTc                           <  1:245245/139‑1 (MQ=255)                                                                                                          tGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCa                          >  1:527239/1‑140 (MQ=255)                                                                                                          tGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCa                          >  1:1426258/1‑140 (MQ=255)                                                                                                          tGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCa                          >  1:330275/1‑140 (MQ=255)                                                                                                          tGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGGTAAAATATTTACAACTTAGCAATCAAACACTAACGCTTGATATCGCTTTTAAAGTTGCGtttttta                          >  2:311715/1‑138 (MQ=255)                                                                                                           tttAATGGTTTTTTTTTTTACATTTTTTTGTGTTTAGGAATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                         <  1:275218/139‑1 (MQ=255)                                                                                                           tttAATGGTTTTTTTTTTTAAATTTTTTTGTTTTTAGGAATAAAAAAACCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                         <  2:552868/139‑1 (MQ=255)                                                                                                           tttAATGGTTTTTTTTTTTAAATTTTTTTGTGTTAAGACATAAAAAAACCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                         <  2:564940/139‑1 (MQ=255)                                                                                                           tttAATGGTTTTTATTTTTACAATTTTTTGTTTTTAGGAATAAAAAAATCATCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                         <  2:154831/139‑1 (MQ=255)                                                                                                           tttAATGGTTTTTATTTTTACAATTTTTTGTGTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                         <  1:665337/139‑1 (MQ=255)                                                                                                           tttAATGGATTTTTTTTTTACATTTTTTTGTTTTTAGGAAAAAAAAAACCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                         <  2:767045/139‑1 (MQ=255)                                                                                                           gTTAATGGTTTTTATTTTTACAATTTTTTGTTTTTAGGCATATAAAAATCAACCCGCCATACGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                         <  1:767937/140‑1 (MQ=255)                                                                                                           gTTAATGGATTTTTTTTTTAAAATTTTTTGTGTTTAGGAATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                         <  1:369136/140‑1 (MQ=255)                                                                                                            ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCata                        >  1:551403/1‑140 (MQ=255)                                                                                                            ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCata                        >  1:312345/1‑140 (MQ=255)                                                                                                            ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCata                        >  1:586585/1‑140 (MQ=255)                                                                                                            ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAATCGCGGTTTTCata                        >  2:1128072/1‑140 (MQ=255)                                                                                                            ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAAAGCTTGATATCGCTTTTAAAATCGCGTTTTTCata                        >  2:1265210/1‑140 (MQ=255)                                                                                                              aatgttttttttttttAAATTTTTTTGTTTTTAGGAATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATc                      <  2:535863/134‑1 (MQ=255)                                                                                                              aaTGGATTTTTTTTTTACATTTTTTTTTTTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATc                      <  2:156287/140‑1 (MQ=255)                                                                                                              aaTGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATc                      <  1:1198028/140‑1 (MQ=255)                                                                                                                   attttattttTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGta                 >  1:883315/1‑140 (MQ=255)                                                                                                                              aaatttttttGTTTTTAGGAATATAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGAcg      <  2:551403/136‑1 (MQ=255)                                                                                                                                aaTTTTTTGTGTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGAcgcg    <  1:1337763/140‑1 (MQ=255)                                                                                                                                  ttttttGTTTTTAGGAATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGa  <  2:853779/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    ATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGA  >  NC_000913/752977‑753245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑