Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,303,668	C→G	P257R (CCG→CGG)	oppB →	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,303,668	0	C	G	100.0%	309.1 / NA	80	P257R (CCG→CGG)	oppB	murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (44/36);  total (44/36)

AAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCC  >  NC_000913/1303532‑1303803                                                                                                                                         |                                                                                                                                        aaaGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAACCCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCgggg                                                                                                                                      <  1:1182601/140‑1 (MQ=255) aaaGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCgggg                                                                                                                                      <  2:640179/140‑1 (MQ=255)   aGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGa                                                                                                                                    >  2:514639/1‑140 (MQ=255)   aGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGa                                                                                                                                    >  1:515424/1‑140 (MQ=255)   aGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGa                                                                                                                                    <  1:942980/140‑1 (MQ=255)        tACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGa                                                                                                                               >  1:897917/1‑140 (MQ=255)         aCCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGAc                                                                                                                              <  1:514639/140‑1 (MQ=255)         aCCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGAc                                                                                                                              <  2:1066403/140‑1 (MQ=255)            tATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAAc                                                                                                                           <  2:897917/140‑1 (MQ=255)               gcggcgGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGt                                                                                                                        >  2:240451/1‑140 (MQ=255)               gcggcgGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGt                                                                                                                        >  2:733357/1‑140 (MQ=255)                  gcggATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCg                                                                                                                     <  1:375551/140‑1 (MQ=255)                   cggATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGt                                                                                                                    >  1:468788/1‑140 (MQ=255)                      aTCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTaa                                                                                                                 <  1:970701/140‑1 (MQ=255)                                gTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTg                                                                                                       <  1:240451/140‑1 (MQ=255)                                   aCGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAAc                                                                                                    <  1:731734/140‑1 (MQ=255)                                   aCGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAAc                                                                                                    <  2:1258879/140‑1 (MQ=255)                                    cGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAAcc                                                                                                   <  1:1458232/140‑1 (MQ=255)                                    cGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAAcc                                                                                                   >  1:965953/1‑140 (MQ=255)                                      cATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGt                                                                                                 <  2:965953/140‑1 (MQ=255)                                      cATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGt                                                                                                 <  1:733357/140‑1 (MQ=255)                                           aaaCCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTa                                                                                            <  2:1169461/140‑1 (MQ=255)                                           aaaCCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTa                                                                                            <  1:1321554/140‑1 (MQ=255)                                             aCCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTAtt                                                                                          <  2:468788/140‑1 (MQ=255)                                                 gCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCtt                                                                                      <  1:408080/140‑1 (MQ=255)                                                 gCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCtt                                                                                      >  2:769787/1‑140 (MQ=255)                                                 gCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCtt                                                                                      >  1:1285282/1‑140 (MQ=255)                                                 gCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCtt                                                                                      <  2:1211503/140‑1 (MQ=255)                                                      gTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAATCGTGACTATTCCTTAgtgt                                                                                 <  1:735790/140‑1 (MQ=255)                                                       ttACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGa                                                                                               >  1:1236224/1‑125 (MQ=255)                                                       ttACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGa                                                                                               <  2:1236224/125‑1 (MQ=255)                                                          cccGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAg                                                                             <  2:45880/140‑1 (MQ=255)                                                             gTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCt                                                                          >  2:1041561/1‑140 (MQ=255)                                                              tGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTg                                                                         >  1:1192376/1‑140 (MQ=255)                                                               gCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAgtg                                                                                  <  1:622916/130‑1 (MQ=255)                                                               gCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAgtg                                                                                  >  2:622916/1‑130 (MQ=255)                                                               gCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGa                                                                        >  1:636723/1‑140 (MQ=255)                                                               gCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGa                                                                        >  2:1244681/1‑140 (MQ=255)                                                               gCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGa                                                                        <  2:974807/140‑1 (MQ=255)                                                               gCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGa                                                                        >  1:404590/1‑140 (MQ=255)                                                               gCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGa                                                                        >  2:461472/1‑140 (MQ=255)                                                                    ctTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATc                                                                   <  2:636723/140‑1 (MQ=255)                                                                     ttATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGa                                                                        >  1:738444/1‑134 (MQ=255)                                                                     ttATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGa                                                                        <  2:738444/134‑1 (MQ=255)                                                                             gCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGAc                                                                       <  1:242722/127‑1 (MQ=255)                                                                             gCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGAc                                                                       >  2:242722/1‑127 (MQ=255)                                                                             gCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCtggttggt                                                          >  1:421050/1‑140 (MQ=255)                                                                             gCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCtggttggt                                                          >  1:1349436/1‑140 (MQ=255)                                                                             gCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCtggttggt                                                          >  1:1245286/1‑140 (MQ=255)                                                                             gCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCtggttggt                                                          >  2:649789/1‑140 (MQ=255)                                                                             gCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCtggttggt                                                          >  2:979378/1‑140 (MQ=255)                                                                               cccGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGc                                                        >  1:325084/1‑140 (MQ=255)                                                                               cccGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGc                                                        >  1:1170593/1‑140 (MQ=255)                                                                               cccGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGc                                                        >  2:1544142/1‑140 (MQ=255)                                                                                 cGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCtt                                                      >  2:100492/1‑140 (MQ=255)                                                                                 cGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCtt                                                      >  1:1188871/1‑140 (MQ=255)                                                                                 cGCATTTGTCGGCATTATTACCGGTTCTACGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCtt                                                      >  1:1066072/1‑140 (MQ=255)                                                                                  gCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCttt                                                     >  1:300095/1‑140 (MQ=255)                                                                                    aTTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTaa                                                   <  1:534191/140‑1 (MQ=255)                                                                                         tCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCAtt                                              <  2:399237/140‑1 (MQ=255)                                                                                         tCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCAtt                                              <  1:769787/140‑1 (MQ=255)                                                                                                 attaCCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTaa                                      <  2:1188871/140‑1 (MQ=255)                                                                                                  ttaCCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCAt                                               <  2:312892/130‑1 (MQ=255)                                                                                                  ttaCCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCAt                                               >  1:312892/1‑130 (MQ=255)                                                                                                  ttaCCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAAt                                     <  1:1546744/140‑1 (MQ=255)                                                                                                     ccGGTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGcc                                  >  2:899247/1‑140 (MQ=255)                                                                                                       ggTTCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCAt                                >  1:628796/1‑140 (MQ=255)                                                                                                          tCTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGt                             >  1:74462/1‑140 (MQ=255)                                                                                                           cTATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTc                            >  2:177917/1‑140 (MQ=255)                                                                                                            tATGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCg                           >  2:51660/1‑140 (MQ=255)                                                                                                              tGGTTATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGAt                         <  2:325084/140‑1 (MQ=255)                                                                                                                 ttATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGAtgtg                      <  2:74462/140‑1 (MQ=255)                                                                                                                  tATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTAAATGCCATTg                              <  1:1059551/131‑1 (MQ=255)                                                                                                                  tATCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTAAATGCCATTg                              >  2:1059551/1‑131 (MQ=255)                                                                                                                   aTCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCAt                                <  1:85018/128‑1 (MQ=255)                                                                                                                   aTCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCAt                                >  2:85018/1‑128 (MQ=255)                                                                                                                   aTCGAAACCATTTATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCt                    >  1:146701/1‑140 (MQ=255)                                                                                                                              ttATGGTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTa         <  2:421050/140‑1 (MQ=255)                                                                                                                                  ggTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGa     >  2:78850/1‑140 (MQ=255)                                                                                                                                  ggTTTGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGa     >  2:1062817/1‑140 (MQ=255)                                                                                                                                     ttGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGAccc  >  2:27045/1‑140 (MQ=255)                                                                                                                                     ttGCGGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTCCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGAccc  >  2:654164/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        AAAGGGTTACCTATGCGGCGGATCATTTTACGTCACGCATTAAAACCTGCTCTGTTACCCGTGCTCTCTTATATGGGCCCCGCATTTGTCGGCATTATTACCGGTTCTATGGTTATCGAAACCATTTATGGTTTGCCGGGGATTGGACAACTGTTCGTTAATGGCGCATTGAACCGTGACTATTCCTTAGTGTTAAGCCTGACCATCCTGGTTGGTGCTTTAACCATTTTGTTTAATGCCATTGTCGATGTGCTATATGCGGTTATCGACCC  >  NC_000913/1303532‑1303803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑