Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,017,423	A→C	E290D (GAA→GAC)	fliI →	flagellum‑specific ATP synthase FliI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,017,423	0	A	C	100.0%	224.7 / NA	59	E290D (GAA→GAC)	fliI	flagellum‑specific ATP synthase FliI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (31/28);  total (31/28)

AGGGTGCCGCCTATGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGAACCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCG  >  NC_000913/2017296‑2017550                                                                                                                                |                                                                                                                                aGGTTGCCGCCTATGCCACGCGCATTGCGGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGccacc                                                                                                                     <  2:1532801/140‑1 (MQ=255) aGGGTGCCGCCTATGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGccacc                                                                                                                     <  1:1434032/140‑1 (MQ=255)   ggTGCCGCCTATGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCaa                                                                                                                   <  1:252473/140‑1 (MQ=255)    gTGCCGCCTATGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCaaa                                                                                                                  >  2:287618/1‑140 (MQ=255)      gccgccTATGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCCCCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAgg                                                                                                                <  2:507322/140‑1 (MQ=255)        cgccTATGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGtt                                                                                                              >  1:1377675/1‑140 (MQ=255)           cTATGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATc                                                                                                           <  2:210527/140‑1 (MQ=255)             aTGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCaa                                                                                                                   <  2:626305/130‑1 (MQ=255)             aTGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCaa                                                                                                                   >  1:626305/1‑130 (MQ=255)             aTGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCa                                                                                                         >  2:1309573/1‑140 (MQ=255)               gCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCAcc                                                                                                       >  1:1532255/1‑140 (MQ=255)                    gcgcATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCgg                                                                                                  >  2:563267/1‑140 (MQ=255)                                  aTTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTa                                                                                    >  1:1027730/1‑140 (MQ=255)                                      tCGCGATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCgg                                                                                <  2:1351003/140‑1 (MQ=255)                                       cgcgATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGc                                                                               <  1:118881/140‑1 (MQ=255)                                       cgcgATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGc                                                                               <  1:1585276/140‑1 (MQ=255)                                        gcgATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCa                                                                              <  1:800265/140‑1 (MQ=255)                                         cgATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCAc                                                                             <  2:973878/140‑1 (MQ=255)                                                 gTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAg                                                                     <  2:1532255/140‑1 (MQ=255)                                                      cATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGc                                                                >  2:884649/1‑140 (MQ=255)                                                                 gATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCa                                                     >  1:1301107/1‑140 (MQ=255)                                                                   ttATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCAtt                                                   >  1:57594/1‑140 (MQ=255)                                                                    tATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTa                                                  >  1:1450280/1‑140 (MQ=255)                                                                             ccTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAgcggcggcg                                         >  2:1442526/1‑140 (MQ=255)                                                                             ccTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAgcggcggcg                                         >  1:88965/1‑140 (MQ=255)                                                                             ccTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAgcggcggcg                                         >  1:356186/1‑140 (MQ=255)                                                                              cTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGcggcggcgg                                        >  2:1457389/1‑140 (MQ=255)                                                                                     gCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGAtt                                 >  1:382742/1‑140 (MQ=255)                                                                                     gCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGAtt                                 <  2:1292752/140‑1 (MQ=255)                                                                                       tACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTAc                               >  2:963613/1‑140 (MQ=255)                                                                                       tACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTAc                               >  2:922584/1‑140 (MQ=255)                                                                                       tACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTAc                               >  1:352049/1‑140 (MQ=255)                                                                                       tACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTAc                               >  1:969888/1‑140 (MQ=255)                                                                                       tACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTAc                               >  2:842394/1‑140 (MQ=255)                                                                                       tACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTAc                               >  1:1095882/1‑140 (MQ=255)                                                                                       tACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTAc                               >  1:1278780/1‑140 (MQ=255)                                                                                       tACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTAc                               >  1:1471315/1‑140 (MQ=255)                                                                                       tACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCG‑ACCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTAcc                              <  1:1573425/140‑1 (MQ=255)                                                                                        aCGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTAcc                              <  1:842394/140‑1 (MQ=255)                                                                                        aCGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTAcc                              <  2:1013946/140‑1 (MQ=255)                                                                                        aCGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTAcc                              <  2:1471315/140‑1 (MQ=255)                                                                                        aCGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTAcc                              <  1:38448/140‑1 (MQ=255)                                                                                        aCGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTAcc                              <  2:622277/140‑1 (MQ=255)                                                                                        aCGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTAcc                              <  2:474631/140‑1 (MQ=255)                                                                                        aCGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTAcc                              <  2:352049/140‑1 (MQ=255)                                                                                         cgcgATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACcg                             >  1:222472/1‑140 (MQ=255)                                                                                         cgcgATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACcg                             >  2:800370/1‑140 (MQ=255)                                                                                         cgcgATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACcg                             >  2:1217414/1‑140 (MQ=255)                                                                                          gcgATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACcgc                            <  1:1309573/140‑1 (MQ=255)                                                                                             aTGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGtt                         <  2:34780/140‑1 (MQ=255)                                                                                                 cccAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTtat                     >  1:1414389/1‑140 (MQ=255)                                                                                                 cccAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTtat                     >  1:1038519/1‑140 (MQ=255)                                                                                                  ccAGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTtata                    <  1:884649/140‑1 (MQ=255)                                                                                                    aGCGTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATAcc                  <  1:1320595/140‑1 (MQ=255)                                                                                                       gTGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTg               <  1:1027855/140‑1 (MQ=255)                                                                                                        tGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGtttt                       >  2:323545/1‑131 (MQ=255)                                                                                                        tGAAATTGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGtttt                       <  1:323545/131‑1 (MQ=255)                                                                                                              tGCGCTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTg        <  2:1414389/140‑1 (MQ=255)                                                                                                                  cTGGCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAgg    >  1:275436/1‑140 (MQ=255)                                                                                                                    ggCGATTGGCGACCCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCg  <  2:1095882/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                AGGGTGCCGCCTATGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGTGTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGAACCCCCCGCCACCAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGCTCGATTACCGCGTTTTATACCGTGCTCACTGAAGGCG  >  NC_000913/2017296‑2017550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑