Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	454,209	T→C	intergenic (‑42/‑263)	yajG ← / → bolA	putative lipoprotein YajG/DNA‑binding transcriptional dual regulator BolA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	454,209	0	T	C	100.0%	363.1 / NA	94	intergenic (‑42/‑263)	yajG/bolA	putative lipoprotein YajG/DNA‑binding transcriptional dual regulator BolA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (49/45);  total (49/45)

TGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATTCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAACACTGATGCGAGGCCTGTGTTTCAATCTTTAAATCAGTAAA  >  NC_000913/454072‑454342                                                                                                                                          |                                                                                                                                      tgtAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATccc                                                                                                                                     >  1:596650/1‑140 (MQ=255) tgtAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATccc                                                                                                                                     >  2:1406372/1‑140 (MQ=255)  gtAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATcccc                                                                                                                                    <  2:948760/140‑1 (MQ=255)  gtAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATcccc                                                                                                                                    <  1:155782/140‑1 (MQ=255)   tAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCg                                                                                                                                   <  1:684057/140‑1 (MQ=255)   tAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCg                                                                                                                                   <  1:638338/140‑1 (MQ=255)   tAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCg                                                                                                                                   >  2:932888/1‑140 (MQ=255)    aaTCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGc                                                                                                                                  <  1:776288/140‑1 (MQ=255)    aaTCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGc                                                                                                                                  <  1:135362/140‑1 (MQ=255)    aaTCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGc                                                                                                                                  <  2:1062674/140‑1 (MQ=255)    aaTCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGc                                                                                                                                  <  2:1248628/140‑1 (MQ=255)           ggggAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCcat                                                                                                                               <  2:970487/136‑1 (MQ=255)           ggggAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCcat                                                                                                                               <  2:468031/136‑1 (MQ=255)           ggggAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCcat                                                                                                                               >  1:468031/1‑136 (MQ=255)           ggggAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCcat                                                                                                                               >  1:970487/1‑136 (MQ=255)             ggAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATaaa                                                                                                                         >  2:1191782/1‑139 (MQ=255)             ggAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAAc                                                                                                                         >  1:756508/1‑140 (MQ=255)             ggAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAAc                                                                                                                         >  1:1475643/1‑140 (MQ=255)             ggAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAAc                                                                                                                         >  2:745460/1‑140 (MQ=255)                   ttCAATAGTTGTTGGCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGcc                                                                                                                   <  1:653516/140‑1 (MQ=255)                   ttCAATAGTTGTTGGCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGcc                                                                                                                   <  2:168116/140‑1 (MQ=255)                   ttCAATAGTTGTTGGCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGcc                                                                                                                   <  1:276441/140‑1 (MQ=255)                            tgttgGCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAACTCCCCGCCATCATAACATTGCCAACGGCGAg                                                                                                          <  2:643760/140‑1 (MQ=255)                            tgttgGCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAg                                                                                                          >  1:997083/1‑140 (MQ=255)                            tgttgGCGGTTTTGCGAATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAg                                                                                                          <  1:1304603/140‑1 (MQ=255)                             gttgGCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAgg                                                                                                         >  1:1359418/1‑140 (MQ=255)                                ggCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGa                                                                                                      <  1:116145/140‑1 (MQ=255)                                 gCGTTTTTGCGCATCCTGCAAGCATAAACGGAGTATCTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTGGGAGAGACTGTTAAGCGTGTTTGGTAAAATTCCCCGCCATCATAACATTGCCAACGGCGAGGGGaa                                                                                                     <  2:718026/140‑1 (MQ=255)                                 gCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGaa                                                                                                     <  1:1592422/140‑1 (MQ=255)                                 gCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGaa                                                                                                     <  1:793221/140‑1 (MQ=255)                                 gCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGaa                                                                                                     <  1:1286680/140‑1 (MQ=255)                                 gCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGaa                                                                                                     <  1:195423/140‑1 (MQ=255)                                     ttttGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTgg                                                                                                 >  2:796503/1‑140 (MQ=255)                                     ttttGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTgg                                                                                                 >  1:55331/1‑140 (MQ=255)                                       ttGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGt                                                                                               <  2:1475643/140‑1 (MQ=255)                                       ttGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGt                                                                                               <  1:516928/140‑1 (MQ=255)                                         gcgcATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTaa                                                                                             <  1:131889/140‑1 (MQ=255)                                                 tGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAgg                                                                                           >  1:14406/1‑134 (MQ=255)                                                 tGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAgg                                                                                           <  2:14406/134‑1 (MQ=255)                                                 tGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTa                                                                                     >  2:58125/1‑140 (MQ=255)                                                         tAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTTCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATtcatc                                                                             >  2:1172691/1‑140 (MQ=255)                                                         tAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATtcatc                                                                             >  2:1449124/1‑140 (MQ=255)                                                         tAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATtcatc                                                                             >  1:363049/1‑140 (MQ=255)                                                         tAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCAGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATtcatc                                                                             >  2:537375/1‑140 (MQ=255)                                                           aaCAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTcatcat                                                                           >  1:547865/1‑140 (MQ=255)                                                           aaCAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTcatcat                                                                           >  1:1557903/1‑140 (MQ=255)                                                               gagCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTg                                                                       <  2:411010/140‑1 (MQ=255)                                                                agCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGa                                                                      >  1:858999/1‑140 (MQ=255)                                                                agCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGa                                                                      >  2:523930/1‑140 (MQ=255)                                                                agCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGa                                                                      >  1:378613/1‑140 (MQ=255)                                                                agCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGa                                                                      <  1:1417963/140‑1 (MQ=255)                                                                agCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGa                                                                      >  1:109212/1‑140 (MQ=255)                                                                agCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGa                                                                      >  2:1247772/1‑140 (MQ=255)                                                                agCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGa                                                                      >  1:1026392/1‑140 (MQ=255)                                                                 gCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGAc                                                                     >  1:1418491/1‑140 (MQ=255)                                                                 gCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAAGCATGTAAATTCATCATGTTGAc                                                                     >  2:1487676/1‑140 (MQ=255)                                                                   aaCTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGa                                                                   <  2:547865/140‑1 (MQ=255)                                                                     cTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGaaa                                                                 >  1:1036125/1‑140 (MQ=255)                                                                     cTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGaaa                                                                 >  1:454580/1‑140 (MQ=255)                                                                      tAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAaat                                                                >  2:837912/1‑140 (MQ=255)                                                                         cGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAGGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAaataat                                                             <  2:1418097/140‑1 (MQ=255)                                                                         cGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAaataat                                                             <  1:1329243/140‑1 (MQ=255)                                                                          gggAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATc                                                            >  2:978670/1‑140 (MQ=255)                                                                          gggAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATc                                                            >  2:946582/1‑140 (MQ=255)                                                                               gagGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGcccc                                                       >  2:1253714/1‑140 (MQ=255)                                                                               gagGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGcccc                                                       <  1:745460/140‑1 (MQ=255)                                                                                        tttGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAg                                              <  1:932888/140‑1 (MQ=255)                                                                                        tttGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAg                                              <  2:363049/140‑1 (MQ=255)                                                                                         ttGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGa                                             <  1:978670/140‑1 (MQ=255)                                                                                           gAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGaaa                                           <  2:1418491/140‑1 (MQ=255)                                                                                            aaCATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGTGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAac                                          >  2:1144400/1‑140 (MQ=255)                                                                                            aaCATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAac                                          >  1:528821/1‑140 (MQ=255)                                                                                             aCATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAaca                                         <  1:1247772/140‑1 (MQ=255)                                                                                             aCATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAaca                                         >  1:428136/1‑140 (MQ=255)                                                                                               aTGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAACACt                                       <  2:997083/140‑1 (MQ=255)                                                                                                     gggCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGTGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAACACTGATGCg                                 <  1:1144400/140‑1 (MQ=255)                                                                                                         tctcAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAACACTGATGCGAGGc                             <  1:1406372/140‑1 (MQ=255)                                                                                                           tcAGAGACTCTTAAGGGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAACACTGATGCGAGGCCt                           <  2:72288/140‑1 (MQ=255)                                                                                                           tcAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAACACTGATGCGAGGCCt                           <  2:1557903/140‑1 (MQ=255)                                                                                                            cAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAACACTGATGCGAGGCCtg                          >  1:1174401/1‑140 (MQ=255)                                                                                                            cAGAGACTCTTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAACACTGATGCGAGGCCtg                          >  2:1330754/1‑140 (MQ=255)                                                                                                                  ctctTAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAACACTGATGCGAGGCCTGTGTTTc                    >  1:593715/1‑140 (MQ=255)                                                                                                                   tctTAAGCGTGTTTGGTAAAAATCCCAGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAACACTGATGCGAGGCCTGTGTTTCa                   <  1:537375/140‑1 (MQ=255)                                                                                                                     ttAAGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAACACTGATGCGAGGCCTGTGTTTCAAt                 >  2:343488/1‑140 (MQ=255)                                                                                                                        aGCGTGTTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAACACTGATGCGAGGCCTGTGTTTCAATCtt              <  2:1174401/140‑1 (MQ=255)                                                                                                                             gtTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAACACTGATGCGAGGCCTGTGTTTCAATCTTTAAAt         >  1:1327573/1‑140 (MQ=255)                                                                                                                             gtTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAACACTGATGCGAGGCCTGTGTTTCAATCTTTAAAt         >  2:900081/1‑140 (MQ=255)                                                                                                                             gtTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAACACTGATGCGAGGCCTGTGTTTCAATCTTTAAAt         >  1:49693/1‑140 (MQ=255)                                                                                                                             gtTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAACACTGATGCGAGGCCTGTGTTTCAATCTTTAAAt         >  2:264897/1‑140 (MQ=255)                                                                                                                             gtTTGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAACACTGATGCGAGGCCTGTGTTTCAATCTTTAAAt         >  2:206760/1‑140 (MQ=255)                                                                                                                              tttGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAACACTGATGCGAGGCCTGTGTTTCAATCTTTAAATc        >  2:677990/1‑140 (MQ=255)                                                                                                                              tttGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAACACTGATGCGAGGCCTGTGTTTCAATCTTTAAATc        >  1:927196/1‑140 (MQ=255)                                                                                                                               ttGGTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAACACTGATGCGAGGCCTGTGTTTCAATCTTTAAATCa       >  2:1547775/1‑140 (MQ=255)                                                                                                                                 ggTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAACACTGATGCGAGGCCTGTGTTTCAATCTTTAAATCAGt     <  2:1323746/140‑1 (MQ=255)                                                                                                                                  gTAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAACACTGATGCGAGGCCTGTGTTTCAATCTTTAAATCAGTa    <  2:593715/140‑1 (MQ=255)                                                                                                                                   tAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAACACTGATGCGAGGCCTGTGTTTCAATCTTTAAATCAGTaa   >  2:592025/1‑140 (MQ=255)                                                                                                                                   tAAAAATCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAACACTGATGCGAGGCCTGTGTTTCAATCTTTAAATCAGTaa   <  2:1051632/140‑1 (MQ=255)                                                                                                                                    aaaaaTCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAACACTGATGCGAGGCCTGTGTTTCAATCTTTAAATCAGTaaa  <  1:1253714/140‑1 (MQ=255)                                                                                                                                    aaaaaTCCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAACACTGATGCGAGGCCTGTGTTTCAATCTTTAAATCAGTaaa  <  1:1419434/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      TGTAATCGTCGGGGAAACTTCAATAGTTGTTGGCGGTTTTGCGCATCCTGCAAGCATAAACAGAGCAACTAACGGGAAGAGGATTTTTTTGAACATGTTCGGGCTCTCAGAGACTCTTAAGCGTGTTTGGTAAAAATTCCCGCCATCATAACATTGCCAACGGCGAGGGGAAGTGGGTAAGGCATGTAAATTCATCATGTTGACGAAATAATCGCCCCTGGTAAAAGAAACACTGATGCGAGGCCTGTGTTTCAATCTTTAAATCAGTAAA  >  NC_000913/454072‑454342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑