Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,981,083	Δ1 bp	coding (530/1425 nt)	otsA ←	trehalose‑6‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,981,081	0	A	.	100.0%	335.2 / NA	78	coding (532/1425 nt)	otsA	trehalose‑6‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (44/34);  total (44/34)

AGACTTCTGTTCGAAATGCTTTGCCCCAGGCTGTATGGCTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCACAAAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCACTCCCCGTTTGCGTAATTCATGCGCAAATGGCAACA  >  NC_000913/1980943‑1981212                                                                                                                                           |                                                                                                                                    aGACTTCTGTTCGAAATGCTTTGCCCCAGGCTGTATGGCTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCACaa                                                                                                                                    <  2:23016/140‑1 (MQ=255)  gACTTCTGTTCGAAATGCTTTGCCCCAGGCTGTATGGCTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCACaag                                                                                                                                   <  1:38130/140‑2 (MQ=255)  gACTTCTGTTCGAAATGCTTTGCCCCAGGCTGTATGGCTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCACaag                                                                                                                                   <  2:2285265/140‑2 (MQ=255)   aCTTCTGTTCGAAATGCTTTGCCCCAGGCTGTATGGCTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCACaagc                                                                                                                                  >  1:1921260/1‑138 (MQ=255)         gTTCGAAATGCTTTGCCCCAGGCTGTATGGCTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTca                                                                                                                           >  1:207879/1‑140 (MQ=255)          ttCGAAATGCTTTGCCCCAGGCTGTATGGCTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTcaa                                                                                                                          >  2:1706055/1‑140 (MQ=255)           tCGAAATGCTTTGCCCCAGGCTGTATGGCTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTcaag                                                                                                                         <  1:1393459/140‑1 (MQ=255)            cGAAATGCTTTGCCCCAGGCTGTATGGCTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTcaagc                                                                                                                        <  2:1246403/140‑1 (MQ=255)            cGAAATGCTTTGCCCCAGGCTGTATGGCTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTcaagc                                                                                                                        <  1:1921862/140‑1 (MQ=255)            cGAAATGCTTTGCCCCAGGCTGTATGGCTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTcaagc                                                                                                                        >  1:1516987/1‑140 (MQ=255)            cGAAATGCTTTGCCCCAGGCTGTATGGCTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTcaagc                                                                                                                        >  1:965093/1‑140 (MQ=255)             gAAATGCTTTGCCCCAGGCTGTATGGCTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTcaagca                                                                                                                       <  2:2082370/140‑1 (MQ=255)               aaTGCTTTGCCCCAGGCTGTATGGCTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTcaagcaag                                                                                                                     <  1:2143150/140‑1 (MQ=255)               aaTGCTTTGCCCCAGGCTGTATGGCTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTcaagcaag                                                                                                                     <  1:2219354/140‑1 (MQ=255)               aaTGCTTTGCCCCAGGCTGTATGGCTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTcaagcaag                                                                                                                     <  2:53005/140‑1 (MQ=255)               aaTGCTTTGCCCCAGGCTGTATGGCTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTcaagcaag                                                                                                                     <  2:2112831/140‑1 (MQ=255)                       gCCCCAGGCTGTATGGCTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTcaagc                                                                                                                        >  1:654095/1‑129 (MQ=255)                       gCCCCAGGCTGTATGGCTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTcaagc                                                                                                                        <  2:654095/129‑1 (MQ=255)                           cAGGCTGTATGGCTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTcaagc                                                                                                                        <  2:1723952/125‑1 (MQ=255)                           cAGGCTGTATGGCTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTcaagc                                                                                                                        >  1:1723952/1‑125 (MQ=255)                             ggCTGTATGGCTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCgg                                                                                                       >  2:445737/1‑140 (MQ=255)                               cTGTATGGCTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCa                                                                                                     <  2:965093/140‑1 (MQ=255)                                  tATGGCTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAgcg                                                                                                  >  2:2472079/1‑140 (MQ=255)                                  tATGGCTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAgcg                                                                                                  >  1:104331/1‑140 (MQ=255)                                   aTGGCTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAgcgc                                                                                                 >  2:192984/1‑140 (MQ=255)                                       cTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTg                                                                                             >  1:1952155/1‑140 (MQ=255)                                       cTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTg                                                                                             >  1:210377/1‑140 (MQ=255)                                            tGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGAt                                                                                        >  1:940615/1‑140 (MQ=255)                                            tGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGAt                                                                                        >  2:1128368/1‑140 (MQ=255)                                            tGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGAt                                                                                        >  2:92521/1‑140 (MQ=255)                                                cTACGTGTCGTGACGCGGGTCAGGTTAGAACGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCCGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGCGTCCTATGTCGGCAGCGCGTTGAAGATTTcc                                                                                    >  2:54665/1‑140 (MQ=255)                                                cTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTcc                                                                                    >  1:1707794/1‑140 (MQ=255)                                                  aCGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCgg                                                                                  <  1:2472079/140‑1 (MQ=255)                                                   cGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGgt                                                                                 <  2:120923/140‑1 (MQ=255)                                                     tgtCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGgtgt                                                                               <  2:2155604/140‑1 (MQ=255)                                                      gtCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTc                                                                              >  2:2124320/1‑140 (MQ=255)                                                       tCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCg                                                                             >  1:1490576/1‑140 (MQ=255)                                                           gACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGaa                                                                         >  1:111998/1‑140 (MQ=255)                                                             cgcgGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGa                                                                          >  1:2269523/1‑137 (MQ=255)                                                             cgcgGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGa                                                                          <  2:2269523/137‑1 (MQ=255)                                                                     aGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGc                                                               <  2:207879/140‑1 (MQ=255)                                                                     aGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGc                                                               <  2:1035092/140‑1 (MQ=255)                                                                       gTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCag                                                             >  2:2172193/1‑140 (MQ=255)                                                                          aGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCagaaa                                                          >  1:1025030/1‑140 (MQ=255)                                                                               gACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAAc                                                     >  1:1063436/1‑140 (MQ=255)                                                                                 cAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCa                                                   >  2:1367367/1‑140 (MQ=255)                                                                                 cAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCa                                                   >  1:1849790/1‑140 (MQ=255)                                                                                 cAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCa                                                   >  1:2403691/1‑140 (MQ=255)                                                                                    tCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATg                                                >  2:2455476/1‑140 (MQ=255)                                                                                    tCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATg                                                <  2:1933340/140‑1 (MQ=255)                                                                                     ccAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCaa                                                  <  2:1194632/137‑1 (MQ=255)                                                                                     ccAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCaa                                                  >  1:1194632/1‑137 (MQ=255)                                                                                          aaCGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGatta                                          >  2:110526/1‑140 (MQ=255)                                                                                              ccAGGCGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGattatt                                        >  1:361636/1‑138 (MQ=255)                                                                                              ccAGGCGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGattatt                                        <  2:361636/138‑1 (MQ=255)                                                                                              ccAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCa                                      >  1:2035723/1‑140 (MQ=255)                                                                                              ccAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCa                                      >  2:200515/1‑140 (MQ=255)                                                                                              ccAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCa                                      >  1:1590391/1‑140 (MQ=255)                                                                                                      tCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCACTCCCCGt                              <  2:111998/140‑1 (MQ=255)                                                                                                       cGTTTTCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCACTCCCCGtt                             >  2:1991453/1‑140 (MQ=255)                                                                                                           ttCTGTCTGGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCACTCCCCGTTTGCg                         >  1:1459076/1‑140 (MQ=255)                                                                                                                tctgGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCACTCCCCGt                              >  2:906181/1‑130 (MQ=255)                                                                                                                tctgGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCACTCCCCGt                              >  2:984706/1‑130 (MQ=255)                                                                                                                tctgGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCACTCCCCGt                              <  1:984706/130‑1 (MQ=255)                                                                                                                tctgGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCACTCCCCGt                              <  1:906181/130‑1 (MQ=255)                                                                                                                tctgGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCACTCCCCGTTTGCGTAAtt                    >  1:1035942/1‑140 (MQ=255)                                                                                                                tctgGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCACTCCCCGTTTGCGTAAtt                    >  1:375790/1‑140 (MQ=255)                                                                                                                tctgGAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCACTCCCCGTTTGCGTAAtt                    >  1:536363/1‑140 (MQ=255)                                                                                                                    gAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCACTCCCCGTTTGCGTAATTCATg                >  2:1771814/1‑140 (MQ=255)                                                                                                                    gAAACCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCACTCCCCGTTTGCGTAATTCATg                <  2:2403691/140‑1 (MQ=255)                                                                                                                      aaCCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCACTCCCCGTTTGCGTAATTCATgcg              <  1:1128368/140‑1 (MQ=255)                                                                                                                      aaCCCAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCACTCCCCGTTTGCGTAATTCATgcg              <  1:1706055/140‑1 (MQ=255)                                                                                                                          cAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCACTCCCCGTTTg                           >  1:2445331/1‑123 (MQ=255)                                                                                                                          cAGCAAATCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCACTCCCCGTTTg                           <  2:2445331/123‑1 (MQ=255)                                                                                                                              aaaTCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCACTCCCCGTTTGCGTAATTCATGCGCAAATGGc      >  1:591551/1‑140 (MQ=255)                                                                                                                              aaaTCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCACTCCCCGTTTGCGTAATTCATGCGCAAATGGc      <  2:1590391/140‑1 (MQ=255)                                                                                                                               aaTCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCACTCCCCGTTTGCGTAATTCATGCGCAAATGGCa     <  2:210377/140‑1 (MQ=255)                                                                                                                                aTCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCACTCCCCGTTTGCGTAATTCATGCGCAAATGGCaa    <  1:1991453/140‑1 (MQ=255)                                                                                                                                aTCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCACTCCCCGTTTGCGTAATTCATGCGCAAATGGCaa    <  1:1771814/140‑1 (MQ=255)                                                                                                                                aTCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCACTCCCCGTTTGCGTAATTCATGCGCAAATGGCaa    >  1:1710572/1‑140 (MQ=255)                                                                                                                                aTCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCACTCCCCGTTTGCGTAATTCATGCGCAAATGGCaa    <  2:2005359/140‑1 (MQ=255)                                                                                                                                aTCATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCACTCCCCGTTTGCGTAATTCATGCGCAAATGGCaa    <  2:2034144/140‑1 (MQ=255)                                                                                                                                  cATAATCAC‑AAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCACTCCCCGTTTGCGTAATTCATGCGCAAATGGCAACa  <  2:1813256/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                    AGACTTCTGTTCGAAATGCTTTGCCCCAGGCTGTATGGCTTTTTGCGCTACGTGTCGTGACGCGGGTCAGGTTAGAAAGACAATCCAGGAACGCCAGACGATCGTTTTCTGTCTGGAAACCCAGCAAATCATAATCACAAAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAACCAATGCGATTATTCACTCCCCGTTTGCGTAATTCATGCGCAAATGGCAACA  >  NC_000913/1980943‑1981212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑