Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT48.3% 82.2 / 111.6 120S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
Reads supporting (aligned to +/- strand):  ref base C (5/57);  new base T (58/0);  total (63/57)
Fisher's exact test for biased strand distribution p-value = 7.90e-29
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 143 total aligned reads displayed.

GCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCA  >  NC_000913/4604209‑4604483
                                                                                                                                       |                                                                                                                                             
acGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttttttt                                                                                                                                           >  1:394683/2‑140 (MQ=255)
 cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttcttttt                                                                                                                                          <  1:1099239/140‑1 (MQ=255)
  gTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttttttttc                                                                                                                                         >  1:1239571/1‑140 (MQ=255)
   tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTttttctttttct                                                                                                                                        <  1:1783411/140‑1 (MQ=255)
   tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTttttctttttct                                                                                                                                        <  2:353323/140‑1 (MQ=255)
   tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTttttctttttct                                                                                                                                        <  1:123744/140‑1 (MQ=255)
          tagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATga                                                                                                                                 <  2:2168755/140‑1 (MQ=255)
            gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCCCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAtttttttttttttt                                                                                                                                        >  1:567418/1‑129 (MQ=255)
            gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCCCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTttttctttttct                                                                                                                                        <  2:567418/131‑1 (MQ=255)
                 ggTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAA‑GTgg                                                                                                                         >  2:2491764/1‑139 (MQ=255)
                  gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAA‑GTGAg                                                                                                                        >  1:1521650/1‑140 (MQ=255)
                  gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGTCATGAGAA‑GTGAg                                                                                                                        >  1:1729778/1‑140 (MQ=255)
                  gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAA‑GTGAg                                                                                                                        >  1:1876425/1‑140 (MQ=255)
                  gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAg                                                                                                                        <  1:1195301/140‑1 (MQ=255)
                  gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTGCCACGTGAA‑GTGAg                                                                                                                        >  2:1643864/1‑140 (MQ=255)
                    aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAA‑GGGAggg                                                                                                                      >  2:2372646/1‑138 (MQ=255)
                    aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAA‑GTGAgcg                                                                                                                      >  2:833811/1‑140 (MQ=255)
                    aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAA‑GTGAgcg                                                                                                                      >  1:242765/1‑140 (MQ=255)
                    aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAA‑GTGAgcg                                                                                                                      >  2:1291348/1‑140 (MQ=255)
                       gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATTAGAA‑GTGAGGGCag                                                                                                                   >  2:1759379/1‑140 (MQ=255)
                       gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAA‑GTgggggccg                                                                                                                   >  2:451084/1‑133 (MQ=255)
                        agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCaga                                                                                                                  <  2:2288135/140‑1 (MQ=255)
                        agagTTTATTCAGAAAGTTTGCGGATAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCaga                                                                                                                  <  2:2341018/140‑1 (MQ=255)
                          agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTTCCTTAGAA‑GGGAGCGCagggg                                                                                                                >  2:594390/1‑137 (MQ=255)
                             ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAA‑GTGAGCGCAGAGAggg                                                                                                             >  2:1770870/1‑140 (MQ=255)
                             ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAA‑GGGAGGGCAGAGAggg                                                                                                             >  1:1227362/1‑140 (MQ=255)
                             ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCCTGAGAA‑GAGAGGGCGGGGAggg                                                                                                             >  2:2369743/1‑140 (MQ=255)
                              tATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGa                                                                                                            <  1:197718/140‑1 (MQ=255)
                               aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAA‑GTGAGCGCAGAGgggggt                                                                                                           >  1:2307211/1‑138 (MQ=255)
                                    gAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCt                                                                                                      >  1:1997525/1‑140 (MQ=255)
                                     aaaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCATTAGAA‑GGGAGCGCAGAGAGGGGTTAtttt                                                                                                     >  1:218248/1‑140 (MQ=255)
                                     aaaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTAATTTTTTTTTTTTTTTCCATATGAAGGTGAG‑GCAGGGAGGGAttttgtt                                                                                                     >  1:1185566/1‑135 (MQ=255)
                                      aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTTCCCGAGGA‑AGGAGCGCCGAGAGGGGTTAttttt                                                                                                    >  2:1054859/1‑139 (MQ=255)
                                      aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTg                                                                                                    <  2:1997525/140‑1 (MQ=255)
                                                 tAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAc                                                                                         <  2:1847617/140‑1 (MQ=255)
                                                   aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTTCATGAGAA‑GGGAGGGCAGAGAGGGGTTTTCTTGCCTGGCTGAACtt                                                                                       >  1:842278/1‑139 (MQ=255)
                                                    cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTgg                                                                                      <  2:798808/140‑1 (MQ=255)
                                                     ccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGc                                                                                     <  1:256595/140‑1 (MQ=255)
                                                         ataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAA‑GCGAGCGGAGAGAGGGATTTTCTTGCCTGACTGAACTGGC‑GGtt                                                                                >  1:1132857/1‑140 (MQ=255)
                                                             gCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGAGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGt                                                                            <  2:2094946/140‑1 (MQ=255)
                                                              cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGtt                                                                           <  2:1083056/140‑1 (MQ=255)
                                                               tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTTATTTTTTTTTTTTTTGCCATGCGAA‑CTTGACGCAGAGAGGGGTTAAATTAACTTGATGAACACGC‑GATTAAGgtt                                                                          >  2:342334/1‑140 (MQ=255)
                                                               tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCCTTAGAA‑GTGAGCGGCGAGAGGGGTTATTTTGCCTTTCTGAACTGGC‑GGTTAAGttt                                                                          >  2:1150545/1‑140 (MQ=255)
                                                               tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTCTTCCCAAAGAA‑GTGAGCGAAAAGAGGGGCTTTCTTTCCTTAGTGAACTTGT‑GATTAACttt                                                                          >  2:1570549/1‑140 (MQ=255)
                                                               tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAA‑GTGAGCGCCGAGAGGGGTTATCTTGTCTGACCGGAATGGG‑GGTTAAGttt                                                                          >  2:1741821/1‑140 (MQ=255)
                                                               tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAA‑GGGAGCGCAGAGAGGGAGTATTTTGCCTGACTGAACTGGC‑GGTTAAAttt                                                                          >  2:962292/1‑140 (MQ=255)
                                                                 cATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTTAGAA‑ATGGGCGCAGAGAGGGAGTATCTTGCCTGACTGAACTAGC‑GATTAAATTTcc                                                                        >  2:47371/1‑140 (MQ=255)
                                                                  aTACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGTTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCcg                                                                       <  2:309934/140‑1 (MQ=255)
                                                                   tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAA‑GTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGC‑GATTAAgctgtctc                                                                      >  2:294553/1‑133 (MQ=255)
                                                                       tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTAc                                                                  <  1:1054859/140‑1 (MQ=255)
                                                                       tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTAc                                                                  <  1:611068/140‑1 (MQ=255)
                                                                        ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTAcc                                                                 <  1:345862/140‑1 (MQ=255)
                                                                         taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAA‑GGGAGCGCAGAGAGGGATTATCTTTCCTGACTGAACTGGC‑GATTTAGTTTCCGCGGAccc                                                                >  2:345862/1‑140 (MQ=255)
                                                                         taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTAccc                                                                <  1:1629337/140‑1 (MQ=255)
                                                                          aCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCg                                                               <  2:780998/140‑1 (MQ=255)
                                                                          aCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCg                                                               <  1:1528228/140‑1 (MQ=255)
                                                                          aCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCg                                                               <  1:395019/140‑1 (MQ=255)
                                                                             aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCGTTTTTTCTTTTTTTGCCGTCGGAA‑GGGAGCGCAGAGAGGGGATATTGTTTCTTGGTTAACGTGC‑GGTTAAGTTTTCGCGGACCCGGcc                                                            >  2:2303617/1‑139 (MQ=255)
                                                                              gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCATGAGAA‑ATGAGCGCAGAGAGGGGTTATTTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCCGCGt                                                           >  2:2059546/1‑140 (MQ=255)
                                                                              gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGt                                                           <  1:2118514/140‑1 (MQ=255)
                                                                               tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGTGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAA‑GTGAGCGCCGAGGGGGGTTATCTTTTCTGACTGAACTTGG‑GGGTAAGTTTTCGCGTACCCGGCggt                                                          >  2:546664/1‑138 (MQ=255)
                                                                               tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCATGAGAA‑GAGAGCGCAGCGGGGGGTTTTTTTTTCTGACCGAAATGGC‑GGTTAAATTTTCCCGTACCCcgcggt                                                          >  2:611068/1‑138 (MQ=255)
                                                                               tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAA‑GGGAGCGCAGAGAGGGGTTATCTTTCCTGACTGAACTGGC‑GATTAAGGTTTCGCGTACCCGGCggt                                                          >  2:1942790/1‑138 (MQ=255)
                                                                               tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTTAGAA‑GGGGGCGCAGAGAGGGGTTATTTTTCCTGGCCGAACTTGG‑GATTTAGTTTCCCACAACCCGGCGtt                                                          >  2:1963187/1‑140 (MQ=255)
                                                                                cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCCTGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGttt                                                         >  2:882361/1‑140 (MQ=255)
                                                                                cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAA‑GTGAGCGCAGAGAGGGGTTATCTTTCCTGACTGAACTGGC‑GATTAAGTTTTCGCGTACCCGGCGgtt                                                         >  2:1628027/1‑140 (MQ=255)
                                                                                cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAA‑GGGAGCGCCGAGAGGGGTTATCTTTCCTGACTGAACTGGC‑GGTTTAGTTTTCGCGGACCCGGCGttt                                                         >  2:2148057/1‑140 (MQ=255)
                                                                                cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAA‑GGGAGCGCAGAGAGGGGTTATTTTTCCTGACTGAACTGGC‑GATTAAGTTTTCGCGGACCCGGCGttt                                                         >  2:2279330/1‑140 (MQ=255)
                                                                                 aGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAA‑GGGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTTCGCGTACCCCGCGgttt                                                        >  2:2118514/1‑139 (MQ=255)
                                                                                 aGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCACGAGAA‑GTGAGCGCCGAGAGGGGTTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTTCGCGGACCCGGCgctgt                                                        >  2:1114950/1‑136 (MQ=255)
                                                                                   gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAA‑GTGAGGGCGGAGAGGGGTTATCTTGCCTGACTGAAATGGC‑GATTAAGTTTTCGCGTACCCGGCGGTTTGt                                                      >  2:2318654/1‑140 (MQ=255)
                                                                                   gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAA‑GTGAGGGCAGAGGGGGGTTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTTCCCGTACCCGGCGTTTTGt                                                      >  2:1130705/1‑140 (MQ=255)
                                                                                   gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGt                                                      <  1:887643/140‑1 (MQ=255)
                                                                                     gttaTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTa                                                    <  2:1002416/137‑1 (MQ=255)
                                                                                        aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTg                                                 <  2:978219/140‑1 (MQ=255)
                                                                                        aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTg                                                 <  1:74981/140‑1 (MQ=255)
                                                                                             aGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCCTGAGAA‑GTGAGCGCAGAGAGGGGGTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTTCGCGTACCCGGCGTTTAGTTTTTTCGgat                                            >  2:130944/1‑140 (MQ=255)
                                                                                               gATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatga                                          <  1:1335486/140‑1 (MQ=255)
                                                                                                aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTTAGGA‑AAGAGAGCAGAGAGGGGTTATCTTTCCTGACCGAACTGGCGGTTTAAGTTT‑CGCGTACCCCGCGGTTTGATATTTCGggtggg                                         >  2:1823536/1‑138 (MQ=255)
                                                                                                aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgat                                         <  1:597030/140‑1 (MQ=255)
                                                                                                aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgat                                         <  1:1459081/140‑1 (MQ=255)
                                                                                                    cATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAA‑GGGAGGGCGGAGAGGGGGTATCTTTCCTGCCTGAAATGGG‑GGTTAAGATTTCCCGTGCCCCGCGGTTTGTTTTTGCGGGTTATGata                                     >  2:597030/1‑140 (MQ=255)
                                                                                                      tGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCCTGAGAA‑GAGAGGGCAGAGAGGGGTTATCTTTCCTGACTGAACTGGC‑GAGTAAAGTTTCGCGTAACCCGCGTTTTGTTTTTGCGGATGGTGATAtt                                   >  2:1167802/1‑140 (MQ=255)
                                                                                                         cgcgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGaa                                <  2:1963490/140‑1 (MQ=255)
                                                                                                         cgcgGATCTCGTTTGCGGCGGGCATTTTTTCTTTTTTTGTCATGGGGA‑AGATGCCCCGGGAGGGAATTTTTTGGCCGACTGAACTAGC‑GATTAAGTTTCCGCGTACCCCGCGGTTAGTTATTGCGGAGGAATATATTTaa                                >  2:1644866/1‑140 (MQ=255)
                                                                                                          gcgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAg                               <  1:130944/140‑1 (MQ=255)
                                                                                                          gcgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAg                               <  1:2036180/140‑1 (MQ=255)
                                                                                                          gcgAATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAg                               <  1:1892106/140‑1 (MQ=255)
                                                                                                               tctcGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCgg                          <  1:1130705/140‑1 (MQ=255)
                                                                                                                   gTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGa                      <  1:2303086/140‑1 (MQ=255)
                                                                                                                    tttGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGAt                     <  1:1741821/140‑1 (MQ=255)
                                                                                                                        cggcggTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGt                 <  2:2143940/140‑1 (MQ=255)
                                                                                                                        cggcggTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGt                 <  1:2491764/140‑1 (MQ=255)
                                                                                                                             gTCCTTTTTTTTTTTTTTTCCATGAGAA‑GTGAGCGCAGAGAGGGGTTATCTTTCCTGTCTGAACTTGC‑GATTAAGTTTCCGCGTACCCCGCGGTTTGTTATTGCGGGTGATGATATTTAAGCTCGGCTGATTGGTTCAtt            >  2:239503/1‑140 (MQ=255)
                                                                                                                                aTTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTc         <  1:1291348/140‑1 (MQ=255)
                                                                                                                                     ttctttttctGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt    <  1:1942790/140‑1 (MQ=255)
                                                                                                                                     ttctttttctGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt    <  1:2266367/140‑1 (MQ=255)
                                                                                                                                     ttctttttctGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt    <  1:546664/140‑1 (MQ=255)
                                                                                                                                     ttctttttctGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt    <  1:1080943/140‑1 (MQ=255)
                                                                                                                                       ctttttctGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCa  <  1:2416942/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                             
GCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAA‑GTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGC‑GATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCA  >  NC_000913/4604209‑4604483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑