Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,233,268	C→G	V484L (GTC→CTC)	nhaB ←	Na(+):H(+) antiporter NhaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,233,268	0	C	G	100.0%	179.1 / NA	46	V484L (GTC→CTC)	nhaB	Na(+):H(+) antiporter NhaB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (18/28);  total (18/28)

GGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACC  >  NC_000913/1233131‑1233400                                                                                                                                          |                                                                                                                                     gggCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAggagga                                                                                                                                    <  1:1269313/140‑1 (MQ=255) gggCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAggagga                                                                                                                                    <  1:595332/140‑1 (MQ=255)      aaaaGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGgtg                                                                                                                               <  1:1096152/140‑1 (MQ=255)           gCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAgg                                                                                                                          <  2:1117898/140‑1 (MQ=255)           gCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAgg                                                                                                                          <  1:301882/140‑1 (MQ=255)                ccGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAggg                                                                                                                     <  2:1295742/140‑1 (MQ=255)                ccGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAggg                                                                                                                     <  2:307421/140‑1 (MQ=255)                             tGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCAt                                                                                                        <  2:766241/140‑1 (MQ=255)                              gCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATg                                                                                                       >  1:1391590/1‑140 (MQ=255)                               cccGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGc                                                                                                      <  2:846794/140‑1 (MQ=255)                               cccGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGc                                                                                                      <  1:99203/140‑1 (MQ=255)                               cccGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGc                                                                                                      <  2:594500/140‑1 (MQ=255)                               cccGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGc                                                                                                      >  1:1383229/1‑140 (MQ=255)                                ccGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCg                                                                                                     <  2:1391590/140‑1 (MQ=255)                                  ggTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATg                                                                                                       >  2:1079388/1‑136 (MQ=255)                                  ggTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATg                                                                                                       <  1:1079388/136‑1 (MQ=255)                                  ggTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGc                                                                                                   >  2:948900/1‑140 (MQ=255)                                  ggTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGc                                                                                                   >  1:704484/1‑140 (MQ=255)                                   gTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGcc                                                                                                  >  1:1166976/1‑140 (MQ=255)                                          gttATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAagag                                                                                           >  2:1032569/1‑140 (MQ=255)                                             aTCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAgg                                                                                        >  1:724500/1‑140 (MQ=255)                                               cAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCg                                                                                      <  1:377090/140‑1 (MQ=255)                                                        gCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATgg                                                                             >  1:886892/1‑140 (MQ=255)                                                           aTCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGgcgc                                                                          <  1:450234/140‑1 (MQ=255)                                                            tCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGcgcg                                                                         >  2:1038183/1‑140 (MQ=255)                                                              cAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAg                                                                       >  1:1056873/1‑140 (MQ=255)                                                                 cccATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGc                                                                    >  1:223288/1‑140 (MQ=255)                                                                 cccATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGc                                                                    >  2:1292453/1‑140 (MQ=255)                                                                           tAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTcagc                                                          <  2:724500/140‑1 (MQ=255)                                                                           tAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTcagc                                                          <  1:1038183/140‑1 (MQ=255)                                                                            aaaCCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTcagca                                                         <  2:223288/140‑1 (MQ=255)                                                                              aCCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGcagg                                                       <  1:564025/140‑1 (MQ=255)                                                                              aCCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGcagg                                                       <  1:310179/140‑1 (MQ=255)                                                                                cATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGcaggaa                                                     <  1:1013929/140‑1 (MQ=255)                                                                                   tCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGcaggaacag                                                  >  2:843080/1‑140 (MQ=255)                                                                                        tACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACg                                             <  2:1056873/140‑1 (MQ=255)                                                                                             gggCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGcc                                        >  1:654402/1‑140 (MQ=255)                                                                                               gCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCAATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTg                                      >  2:369639/1‑140 (MQ=255)                                                                                                cAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGa                                     >  1:496384/1‑140 (MQ=255)                                                                                                    cGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGt                                 <  2:532095/140‑1 (MQ=255)                                                                                                             gACGCAGAGCAAGCCGACGAGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAg                        <  1:531846/140‑1 (MQ=255)                                                                                                                            gacgaGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGAt         <  1:1009061/140‑1 (MQ=255)                                                                                                                             acgaGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGAtt        <  2:925893/140‑1 (MQ=255)                                                                                                                              cgaGTGTCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTg       >  2:949459/1‑140 (MQ=255)                                                                                                                                 gtgtCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTa    <  1:949459/140‑1 (MQ=255)                                                                                                                                   gtCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTAcc  <  2:496384/140‑1 (MQ=255)                                                                                                                                   gtCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTAcc  <  2:1383229/140‑1 (MQ=255)                                                                                                                                   gtCAGGAGGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTAcc  <  1:1292453/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     GGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACC  >  NC_000913/1233131‑1233400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑