Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,193,436	C→T	L47L (CTG→CTA)	thiF ←	ThiS adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,193,436	0	C	T	100.0%	58.7 / NA	18	L47L (CTG→CTA)	thiF	ThiS adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/6);  total (12/6)

GTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCCGACGCCAGCGCCCGCCAGGTACAGCGCAGCAGGTGTACCCAGCCCGCCCAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAC  >  NC_000913/4193306‑4193566                                                                                                                                   |                                                                                                                                   gTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCCGACGCCAGCGCCCGCTAGGTACAgc                                                                                                                           >  1:505414/1‑140 (MQ=255)      tGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCCGACGCCAGCGCCCGCTAGGTACAGCgcagc                                                                                                                      >  2:44982/1‑140 (MQ=255)         tGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCCGACGCCAGCGCCCGCTAGGTACAGCGCAGCAgg                                                                                                                   <  1:136025/140‑1 (MQ=255)               aCCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCCGACGCCAGCGCCCGCTAGGTACAGCGCAGCAGGTGTAcc                                                                                                             >  1:481899/1‑140 (MQ=255)                          cGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCCGACGCCAGCGCCCGCTAGGTACAGCGCAGCAGGTGTACCCAGCCCGCCCa                                                                                                  <  1:44982/140‑1 (MQ=255)                               gatcgatATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCCGACGCCAGCGCCCGCTAGGTACAGCGCAGCAGGTGTACCCAGCCCGCCCAGACCg                                                                                             <  2:505414/140‑1 (MQ=255)                                            aGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCCGACGCCAGCGCCCGCTAGGTACAGCGCAGCAGGTGTACCCAGCCCGCCCAGACCGATAATCAGCACCt                                                                                >  2:175030/1‑140 (MQ=255)                                                   aaGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCCGACGCCAGCGCCCGCTAGGTACAGCGCAGCAGGTGTACCCAGCCCGCCCAGACCGATAATCAGCACCTGGCTGTc                                                                         >  1:412727/1‑140 (MQ=255)                                                        gATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCCGACGCCAGCGCCCGCTAGGTACAGCGCAGCAGGTGTACCCAGCCCGCCCAGACCGATAATCAGCACCTGGCTGTCGAGCa                                                                    >  1:27551/1‑140 (MQ=255)                                                                   gCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCCGACGCCAGCGCCCGCTAGGTACAGCGCAGCAGGTGTACCCAGCCCGCCCAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTtgctgc                                                         <  2:481899/140‑1 (MQ=255)                                                                                                  tGCCAGTACCAGCGTCCCGACGCCAGCGCCCGCTAGGTACAGCGCAGCAGGTGTACCCAGCCCGCCCAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATAtcg                                       >  1:267126/1‑127 (MQ=255)                                                                                                  tGCCAGTACCAGCGTCCCGACGCCAGCGCCCGCTAGGTACAGCGCAGCAGGTGTACCCAGCCCGCCCAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATAtcg                                       <  2:267126/127‑1 (MQ=255)                                                                                                        tACCAGCGTCCCGACGCCAGCGCCCGCTAGGTACAGCGCAGCAGGTGTACCCAGCCCGCCCAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTggcggc                    >  2:296129/1‑140 (MQ=255)                                                                                                              cGTCCCGACGCCAGCGCCCGCTAGGTACAGCGCAGCAGGTGTACCCAGCCCGCCCAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAAc              >  2:558563/1‑140 (MQ=255)                                                                                                              cGTCCCGACGCCAGCGCCCGCTAGGTACAGCGCAGCAGGTGTACCCAGCCCGCCCAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCACGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAAc              >  2:35478/1‑140 (MQ=255)                                                                                                                   cGACGCCAGCGCCCGCTAGGTACAGCGCAGCAGGTGTACCCAGCCCGCCCAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATa         >  1:523990/1‑140 (MQ=255)                                                                                                                   cGACGCCAGCGCCCGCTAGGTACAGCGCAGCAGGTGTACCCAGCCCGCCCAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATa         <  1:296129/140‑1 (MQ=255)                                                                                                                          aGCGCCCGCTAGGTACAGCGCAGCAGGTGTACCCAGCCCGCCCAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAc  >  1:169941/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                   GTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCCGACGCCAGCGCCCGCCAGGTACAGCGCAGCAGGTGTACCCAGCCCGCCCAGACCGATAATCAGCACCTGGCTGTCGAGCAGTTTTTGCTGCCCGTCCAGAGCGATATCGTCGAGCAGGATTTGGCGGCTATAACGCATAAAGTCAC  >  NC_000913/4193306‑4193566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑