Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,148,168	T→C	G165G (GGT→GGC)	yegD →	HSP70 family protein YegD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,148,168	0	T	C	100.0%	82.3 / NA	23	G165G (GGT→GGC)	yegD	HSP70 family protein YegD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/10);  total (13/10)

CAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAA  >  NC_000913/2148034‑2148300                                                                                                                                       |                                                                                                                                     cagcagGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGcgatga                                                                                                                                 <  2:504641/140‑1 (MQ=255)                     ggATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGCGATGAAGCAAACACCCAGGCGCAAg                                                                                                             <  1:343526/140‑1 (MQ=255)                     ggATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGCGATGAAGCAAACACCCAGGCGCAAg                                                                                                             <  1:380405/140‑1 (MQ=255)                                gcgcAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGCGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGaa                                                                                                  >  1:464112/1‑140 (MQ=255)                                 cgcAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGCGATGAAGCAAACACCCAGGCGCa                                                                                                               <  1:42399/126‑1 (MQ=255)                                 cgcAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGCGATGAAGCAAACACCCAGGCGCa                                                                                                               >  2:42399/1‑126 (MQ=255)                                 cgcAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGCGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAAc                                                                                                 >  1:273305/1‑140 (MQ=255)                                        atgTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGCGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCgcggc                                                                                          <  1:494884/140‑1 (MQ=255)                                                  ttCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGCGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGcc                                                                                >  1:119554/1‑140 (MQ=255)                                                            ggcgcaggcACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGCGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAggg                                                                      <  2:435643/140‑1 (MQ=255)                                                             gcgcaggcACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGCGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGa                                                                     <  2:532380/140‑1 (MQ=255)                                                                 aggcACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGCGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTg                                                                 <  2:3828/140‑1 (MQ=255)                                                                           gCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGCGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGt                                                       >  1:96986/1‑140 (MQ=255)                                                                             cAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGCGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGCGGTATTCCAGTAc                                                     >  1:488767/1‑140 (MQ=255)                                                                                          tCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGCGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCgg                                        <  1:283645/140‑1 (MQ=255)                                                                                          tCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGCGATGAAGCAAACACCCAGGCGAAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCgg                                        <  2:80654/140‑1 (MQ=255)                                                                                            aGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGCGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCggct                                      >  2:354137/1‑140 (MQ=255)                                                                                                        gtcgtcCGATCAACTTCCAGGGGCTGGGCGGCGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTAc                          >  2:58391/1‑140 (MQ=255)                                                                                                            tcCGATCAACTTCCAGGGGCTGGGCGGCGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAg                      <  2:119554/140‑1 (MQ=255)                                                                                                            tcCGATCAACTTCCAGGGGCTGGGCGGCGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAg                      >  1:587203/1‑140 (MQ=255)                                                                                                            tcCGATCAACTTCCAGGGGCTGGGCGGCGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAg                      >  2:386019/1‑140 (MQ=255)                                                                                                                          aGGGGCTGGGCGGCGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGgaa        >  1:482693/1‑140 (MQ=255)                                                                                                                               cTGGGCGGCGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGaaaa   >  2:126863/1‑140 (MQ=255)                                                                                                                                tGGGCGGCGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGTGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGaaaaa  >  1:510111/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                     CAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGTTGCACATTCGCCAGCAGGCGCAGGCACAGCTGCCAGAAGCGATTACTCAGGCGGTGATTGGTCGTCCGATCAACTTCCAGGGGCTGGGCGGTGATGAAGCAAACACCCAGGCGCAAGGGATTCTGGAACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAA  >  NC_000913/2148034‑2148300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑