| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 35,961 | C→T | A68T (GCG→ACG) | caiD ← | crotonobetainyl‑CoA hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 35,961 | 0 | C | T | 84.9% | 76.4 / 6.2 | 33 | A68T (GCG→ACG) | caiD | crotonobetainyl‑CoA hydratase |
| Reads supporting (aligned to +/- strand): ref base C (2/3); new base T (12/16); total (14/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAGTTCAAAGCCGCCGCCAAAGGCATAGCCGTTCACAGCTGCGATAACCGGTTTGTCGAGATTGAAAATTTCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGCTTTTAAATCCCAGCCCGCGGAAAAGAACTTCTCTCCGGCACCGGTAATAATGGCGACACGTAATTGCGGATCGTCACGGAAATTTAGAAATACTTCGCCCATTTCAAAGCTGGTTTTTGCATCAATAGCATT > NC_000913/35826‑36093 | ccagTTCAAAGCCGCCGCCAAAGGCATAGCCGTTCACAGCTGCGATAACCGGTTTGTCGAGATTGAAAATTTCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGTtttt < 1:664226/140‑1 (MQ=255) ttCAAAGCCGCCGCCAAAGGCATAGCCGTTCACAGCTGCGATAACCGGTTTGTCGAGATTGAAAATTTCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGTTTTTTAAt > 2:557938/1‑140 (MQ=255) aggccgccgccAAAGGCATAGCCGTTCACAGCTGCGATAACCGGTTTGTCGAGATTGAAAATTTCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGTTTTTAAATCCCa < 1:359002/138‑1 (MQ=255) gccgccAAAGGCATAGCCGTTCACAGCTGCGATAACCGGTTTGTCGAGATTGAAAATTTCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGTTTTTAAATCCCAGCCcg > 2:269287/1‑140 (MQ=255) gccgccAAAGGCATAGCCGTTCACAGCTGCGATAACCGGTTTGTCGAGATTGAAAATTTCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGTTTTTAAATCCCAGCCcg > 2:243077/1‑140 (MQ=255) aaaGGCATAGCCGTTCACAGCTGCGATAACCGGTTTGTCGAGATTGAAAATTTCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGTTTTTAAATCCCAGCCCGCGGaaa < 1:182077/140‑1 (MQ=255) aGGCATAGCCGTTCACAGCTGCGATAACCGGTTTGTCGAGATTGAAAATTTCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGTTTTTAAATCCCAGCCCGCGGAAAAg < 1:269287/140‑1 (MQ=255) aGCCGTTCACAGCTGCGATAACCGGTTTGTCGAGATTGAAAATTTCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGTTTTTAAATCCCAGCCcg > 1:11933/1‑126 (MQ=255) aGCCGTTCACAGCTGCGATAACCGGTTTGTCGAGATTGAAAATTTCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGTTTTTAAATCCCAGCCcg < 2:11933/126‑1 (MQ=255) tCACAGCTGCGATAACCGGTTTGTCGAGATTGAAAATTTCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGTTTTTAAATCCCAGCCCGCGGAAAAGAACTTCTCTCCg > 2:683144/1‑140 (MQ=255) tCACAGCTGCGATAACCGGTTTGTCGAGATTGAAAATTTCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGTTTTTAAATCCCAGCCCGCGGAAAAGAACTTCTCTCCg > 2:461831/1‑140 (MQ=255) acaGCTGCGATAACCGGTTTGTCGAGATTGAAAATTTCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGTTTTTAAATCCCAGCCCGCGGAAAAGAACTTCTCTCCGGc < 1:92413/140‑1 (MQ=255) aGCTGCGATAACCGGTTTGTCGAGATTGAAAATTTCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGTTTTTAAATCCCAGCCCGCGGAAAAGAACTTCTCTCCGGCAc < 1:243077/140‑1 (MQ=255) gCTGCGATAACCGGTTTGTCGAGATTGAAAATTTCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGTTTTTAAATCCCAGCCCGCGGAAAAGAACTTCTCTCCGGCAcc < 1:620249/140‑1 (MQ=255) cTGCGATAACCGGTTTGTCGAGATTGAAAATTTCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGTTTTTAAATCCCAGCCCGCGGAAAAGAACTTCTCTCCGGCACCg > 2:246581/1‑140 (MQ=255) cTGCGATAACCGGTTTGTCGAGATTGAAAATTTCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGCTTTTAAATCCCAGCCCGCGGAAAAGAACTTCTCTCCGGCACCg > 1:532471/1‑140 (MQ=255) cGGTTTGTCGAGATTGAAAATTTCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGCTTTTAAATCCCAGCCCGCGGAAAAGAACTTCTCTCCGGCACCGGTAATAATgg > 2:22027/1‑140 (MQ=255) tttGTCGAGATTGAAAATTTCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGTTTTTAAATCCCAGCCCGCGGAAAAGAACTTCTCTCCgg < 1:651013/122‑1 (MQ=255) tttGTCGAGATTGAAAATTTCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGTTTTTAAATCCCAGCCCGCGGAAAAGAACTTCTCTCCgg > 2:651013/1‑122 (MQ=255) tGTCGAGATTGAAAATTTCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGTTTTTAAATCCCAGCCCGCGGAAAAGAACTTCTCTCCGGCACCGGTAATAATGGCGaca < 1:683144/140‑1 (MQ=255) tGTCGAGATTGAAAATTTCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGCGTTTAAATCCCAGCCCGCGGAAAAGAACTTCTCTCCGGCACCGGTAATAATGGCGaca < 2:532471/140‑1 (MQ=255) tCGAGATTGAAAATTTCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGCTTTTAAATCCCAGCCCGCGGAAAAGAACTTCTCTCCGGCACCGGTAATAATGGCGACACg < 2:657181/140‑1 (MQ=255) agaTTGAAAATTTCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGTTTTTAAATCCCAGCCCGCGGAAAAGAACTTCTCTCCGGCACCGGTAATAATGGCGACACGTaa < 1:461831/140‑1 (MQ=255) aaaaTTTCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGTTTTTAAATCCCAGCCCGCGGAAAAGAACTTCTCTCCGGCACCGGTAATAATGGCGACACGTAATTGCgg > 1:647918/1‑140 (MQ=255) ttCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGTTTTTTAATCCCAGCCCGCGGAAAAGAACTTCTCTCCGGCACCGGTAATAATGGCGACACGTAATTGCGGATCGt < 1:557938/140‑1 (MQ=255) ttCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGTTTTTAAATCCCAGCCCGCGGAAAAGAACTTCTCTCCGGCACCGGTAATAATGGCGACACGTAATTGCGGATCGt < 2:647918/140‑1 (MQ=255) ttAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGCTTTTAAATCCCAGCCCGCGGAAAAGAACTTCTCTCCGGCACCGGTAATAATGGCGACACGTAATTGCGGATCGTCACgg < 1:22027/140‑1 (MQ=255) aCCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGTTTTTAAATCCCAGCCCGCGGAAAAGAACTTCTCTCCGGCACCGGTAATAATGGCGACACGTAATTGCGGATCGTCACGGAAATTTAGAAATACTTCGc < 2:501417/137‑1 (MQ=255) aCCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGTTTTTAAATCCCAGCCCGCGGAAAAGAACTTCTCTCCGGCACCGGTAATAATGGCGACACGTAATTGCGGATCGTCACGGAAATTTAGAAATACTTCGc > 1:501417/1‑137 (MQ=255) ggTGCTTCGCCTTCTGCTGCCGTTTTTAAATCCCAGCCCGCGGAAAAGAACTTCTCTCCGGCACCGGTAATAATGGCGACACGTAATTGCGGATCGTCACGGAAATTTAGAAATACTTCGCCCATTTCAAAGCTGGtttt < 2:386790/140‑1 (MQ=255) ggTGCTTCGCCTTCTGCTGCCGTTTTTAAATCCCAGCCCGCGGAAAAGAACTTCTCTCCGGCACCGGTAATAATGGCGACACGTAATTGCGGATCGTCACGGAAATTTAGAAATACTTCGCCCATTTCAAAGCTGGtttt > 1:386790/1‑140 (MQ=255) ggTGCTTCGCCTTCTGCTGCCGTTTTTAAATCCCAGCCCGCGGAAAAGAACTTCTCTCCGGCACCGGTAATAATGGCGACACGTAATTGCGGATCGTCACGGAAATTTAGAAATACTTCGCCCATTTCAAAGCTGGtttt > 1:505540/1‑140 (MQ=255) gTGCTTCGCCTTCTGCTGCCGTTTTTAAATCCCAGCCCGCGGAAAAGAACTTCTCTCCGGCACCGGTAATAATGGCGACACGTAATTGCGGATCGTCACGGAAATTTAGAAATACTTCGCCCATTTCAAAGCTGGttttt < 2:505540/140‑1 (MQ=255) cTTTTAAATCCCAGCCCGCGGAAAAGAACTTCTCTCCGGCACCGGTAATAATGGCGACACGTAATTGCGGATCGTCACGGAAATTTAGAAATACTTCGCCCATTTCAAAGCTGGTTTTTGCATCAATAGCAtt > 1:262849/1‑133 (MQ=255) cTTTTAAATCCCAGCCCGCGGAAAAGAACTTCTCTCCGGCACCGGTAATAATGGCGACACGTAATTGCGGATCGTCACGGAAATTTAGAAATACTTCGCCCATTTCAAAGCTGGTTTTTGCATCAATAGCAtt < 2:262849/133‑1 (MQ=255) | CCAGTTCAAAGCCGCCGCCAAAGGCATAGCCGTTCACAGCTGCGATAACCGGTTTGTCGAGATTGAAAATTTCGGTTAATCCCGCAAAACCACCCGGACCAAAGTCAGCATCCGGTGCTTCGCCTTCTGCTGCCGCTTTTAAATCCCAGCCCGCGGAAAAGAACTTCTCTCCGGCACCGGTAATAATGGCGACACGTAATTGCGGATCGTCACGGAAATTTAGAAATACTTCGCCCATTTCAAAGCTGGTTTTTGCATCAATAGCATT > NC_000913/35826‑36093 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |