Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,557,571	T→C	A156A (GCA→GCG)	ddpF ←	putative D,D‑dipeptide ABC transporter ATP‑binding subunit DdpF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,557,571	0	T	C	100.0%	258.4 / NA	67	A156A (GCA→GCG)	ddpF	putative D,D‑dipeptide ABC transporter ATP‑binding subunit DdpF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/33);  total (34/33)

TGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGTGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAACTCCTCTGCCAACGCTCGCCGCTGTTGTTCAC  >  NC_000913/1557435‑1557698                                                                                                                                         |                                                                                                                                tgtgtgAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGct                                                                                                                              <  1:681162/140‑1 (MQ=255) tgtgtgAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGct                                                                                                                              >  2:190887/1‑140 (MQ=255) tgtgtgAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGct                                                                                                                              >  1:601197/1‑140 (MQ=255)   tgtgAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGctct                                                                                                                            >  1:534035/1‑140 (MQ=255)      gAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTgac                                                                                                                         <  1:684963/140‑3 (MQ=255)       aaaTCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCa                                                                                                                        <  1:463951/140‑1 (MQ=255)       aaaTCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCa                                                                                                                        <  1:2047/140‑1 (MQ=255)         aTCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAAt                                                                                                                      >  1:5120/1‑140 (MQ=255)                  tAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACg                                                                                                             <  2:5120/140‑1 (MQ=255)                   aGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGt                                                                                                            <  1:160324/140‑1 (MQ=255)                    ggTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGtt                                                                                                           >  1:688478/1‑140 (MQ=255)                      tCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGa                                                                                                                 >  1:278308/1‑132 (MQ=255)                      tCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGa                                                                                                                 <  2:278308/132‑1 (MQ=255)                        aGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCg                                                                                                       >  2:42436/1‑140 (MQ=255)                        aGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCg                                                                                                       >  1:198892/1‑140 (MQ=255)                        aGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCg                                                                                                       >  1:587848/1‑140 (MQ=255)                           ccGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTg                                                                                                    >  2:6909/1‑140 (MQ=255)                           ccGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTg                                                                                                    >  1:478016/1‑140 (MQ=255)                              tGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGccc                                                                                                 >  1:741563/1‑140 (MQ=255)                                           gCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCa                                                                                    <  2:534035/140‑1 (MQ=255)                                           gCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCa                                                                                    <  1:230245/140‑1 (MQ=255)                                              ttACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGa                                                                                 >  2:323420/1‑140 (MQ=255)                                              ttACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGa                                                                                 >  1:398725/1‑140 (MQ=255)                                                aCCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGagg                                                                               >  2:757342/1‑140 (MQ=255)                                                aCCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGagg                                                                               >  2:693786/1‑140 (MQ=255)                                                     tAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGaggcaggc                                                                          >  1:703121/1‑140 (MQ=255)                                                     tAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGaggcaggc                                                                          <  1:42436/140‑1 (MQ=255)                                                        aTTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGt                                                                       <  2:12931/140‑1 (MQ=255)                                                            aGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCgag                                                                   >  1:501245/1‑140 (MQ=255)                                                             ggATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCgaga                                                                  >  1:141396/1‑140 (MQ=255)                                                               aTCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATa                                                                >  2:574000/1‑140 (MQ=255)                                                               aTCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATa                                                                <  2:741563/140‑1 (MQ=255)                                                                  tGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTc                                                             <  1:702781/140‑1 (MQ=255)                                                                   gcgcCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTcc                                                            >  2:1309/1‑140 (MQ=255)                                                                    cgcCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCg                                                           <  2:807020/140‑1 (MQ=255)                                                                    cgcCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCg                                                           <  1:757342/140‑1 (MQ=255)                                                                      ccTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGa                                                         <  1:512248/140‑1 (MQ=255)                                                                           aCGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAAt                                                    >  1:13965/1‑140 (MQ=255)                                                                                    tCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTg                                           >  2:678314/1‑140 (MQ=255)                                                                                     ccAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGc                                          <  2:131551/140‑1 (MQ=255)                                                                                      cAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGca                                         <  2:198892/140‑1 (MQ=255)                                                                                         cgcAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGAc                                                        >  1:34369/1‑122 (MQ=255)                                                                                         cgcAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGAc                                                        <  2:34369/122‑1 (MQ=255)                                                                                         cgcAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAg                                      >  1:166358/1‑140 (MQ=255)                                                                                         cgcAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAg                                      >  2:278055/1‑140 (MQ=255)                                                                                           cAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGcc                                    <  2:688478/140‑1 (MQ=255)                                                                                            agagGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCa                                   <  1:693786/140‑1 (MQ=255)                                                                                            agagGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCa                                   <  1:443523/140‑1 (MQ=255)                                                                                              agGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAAc                                 >  1:264016/1‑140 (MQ=255)                                                                                                   ggCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAActcctc                            >  2:437963/1‑140 (MQ=255)                                                                                                         tcaAGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAACTCCTCTGCCaa                      >  1:48308/1‑140 (MQ=255)                                                                                                           aaGCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAACTCCTCTGCCAACg                    <  1:276762/140‑1 (MQ=255)                                                                                                             gCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCTACCTGCACAGCCAACTCCTCTGCCAACGCt                  <  1:190887/140‑1 (MQ=255)                                                                                                             gCACAATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAACTCCTCTGCCAACGCt                  <  2:141396/140‑1 (MQ=255)                                                                                                                caATCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAACTCCTCTGCCAACGCTcgc               <  2:501245/140‑1 (MQ=255)                                                                                                                  aTCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAACTCCTCTGCCAACg                    >  2:91070/1‑133 (MQ=255)                                                                                                                  aTCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAACTCCTCTGCCAACg                    <  1:91070/133‑1 (MQ=255)                                                                                                                  aTCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAACTCCTCTGCCAACGCTcgccg             <  2:478016/140‑1 (MQ=255)                                                                                                                  aTCACGTCAGGCTGCGAAGAGAGCGCTCTGGCAACGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAACTCCTCTGCCAACGCTcgccg             >  1:471656/1‑140 (MQ=255)                                                                                                                     aCGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAACTCCTCTGCCAACGCTCGCCGCtg          <  2:264016/140‑1 (MQ=255)                                                                                                                     aCGTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAACTCCTCTGCCAACGCTCGCCGCtg          <  1:437963/140‑1 (MQ=255)                                                                                                                       gTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAACTCCTCTGcc                        >  2:730621/1‑124 (MQ=255)                                                                                                                       gTCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAACTCCTCTGcc                        <  1:730621/124‑1 (MQ=255)                                                                                                                        tCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAACTCCTCTGCCAACGCTCGCCGCtgttg       <  1:278055/140‑1 (MQ=255)                                                                                                                        tCAGGCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAACTCCTCTGCCAACGCTCGCCGCtgttg       >  1:420558/1‑140 (MQ=255)                                                                                                                           ggCTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAACTCCTCTGCCAACCCTCGCCGCTGTTGTTc    >  2:339702/1‑140 (MQ=255)                                                                                                                             cTGCGAAGAGAGCGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAACTCCTCTGCCAACGCTCGCCGCTGTTGTTCac  <  2:601197/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                TGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAAATTGAGGATCTGCGCCTGAACGGAGATATCCAGCGCAGAGGTTGGCTCATCAAGCACAATCACGTCAGGCTGCGAAGAGAGTGCTCTGGCAATGGCGATACGTTGCCGCTGCCCGCCGGAGAACGCATGAGGCAGGCGGTCGAGATACTCCGGACGAATACCCACCTGCACAGCCAACTCCTCTGCCAACGCTCGCCGCTGTTGTTCAC  >  NC_000913/1557435‑1557698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑