Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	933,298	G→A	A25A (GCG→GCA)	ftsK →	cell division DNA translocase FtsK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	933,298	0	G	A	100.0%	254.1 / NA	71	A25A (GCG→GCA)	ftsK	cell division DNA translocase FtsK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/34);  total (37/34)

AACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCGTTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGG  >  NC_000913/933161‑933433                                                                                                                                          |                                                                                                                                        aaCCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCAtt                                                                                                                                       >  2:360951/1‑140 (MQ=255)  aCCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTg                                                                                                                                      >  1:515551/1‑140 (MQ=255)  aCCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTg                                                                                                                                      >  1:374548/1‑140 (MQ=255)  aCCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTg                                                                                                                                      >  1:672516/1‑140 (MQ=255)  aCCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTg                                                                                                                                      >  1:747447/1‑140 (MQ=255)  aCCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTg                                                                                                                                      >  1:727250/1‑140 (MQ=255)    cTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCt                                                                                                                                    <  1:617760/140‑1 (MQ=255)       gTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGAt                                                                                                                                 <  1:806912/140‑1 (MQ=255)       gTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGAt                                                                                                                                 <  2:159802/140‑1 (MQ=255)       gTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGAt                                                                                                                                 <  1:488400/140‑1 (MQ=255)       gTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGAt                                                                                                                                 >  1:189872/1‑140 (MQ=255)        tACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATc                                                                                                                                >  2:522163/1‑140 (MQ=255)             ttgtCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTAt                                                                                                                           >  2:546266/1‑140 (MQ=255)             ttgtCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTAt                                                                                                                           >  2:478736/1‑140 (MQ=255)                 ccGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTc                                                                                                                       >  2:39898/1‑140 (MQ=255)                  cGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCATGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGt                                                                                                                        <  2:376273/138‑1 (MQ=255)                  cGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCATGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGt                                                                                                                        >  1:376273/1‑138 (MQ=255)                     tttAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGt                                                                                                                   >  2:796116/1‑140 (MQ=255)                        aGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTg                                                                                                                <  1:39898/140‑1 (MQ=255)                            tCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGt                                                                                                            <  2:189872/140‑1 (MQ=255)                               ggCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTg                                                                                                         >  2:393701/1‑140 (MQ=255)                               ggCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTg                                                                                                         >  2:347411/1‑140 (MQ=255)                               ggCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTg                                                                                                         >  2:302660/1‑140 (MQ=255)                               ggCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTG‑CCTGTTTGCCGTCTgg                                                                                                        >  1:262005/1‑140 (MQ=255)                                       aaGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATgg                                                                                                 <  2:653965/140‑1 (MQ=255)                                          ccTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTc                                                                                                           >  1:670559/1‑127 (MQ=255)                                          ccTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTc                                                                                                           <  2:670559/127‑1 (MQ=255)                                          ccTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTg                                                                                              <  2:747447/140‑1 (MQ=255)                                          ccTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTg                                                                                              <  1:796116/140‑1 (MQ=255)                                              tAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGc                                                                                             <  1:605407/137‑1 (MQ=255)                                              tAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGc                                                                                             >  2:605407/1‑137 (MQ=255)                                              tAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCtt                                                                                          >  1:345794/1‑140 (MQ=255)                                              tAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCtt                                                                                          >  1:250433/1‑140 (MQ=255)                                              tAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCtt                                                                                          >  2:270675/1‑140 (MQ=255)                                                aCCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTAc                                                                                        <  2:235312/140‑1 (MQ=255)                                                 ccTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACt                                                                                       <  1:546266/140‑1 (MQ=255)                                                 ccTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACt                                                                                       <  2:672516/140‑1 (MQ=255)                                                     gagaGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATgg                                                                                                 >  1:477936/1‑126 (MQ=255)                                                     gagaGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATgg                                                                                                 <  2:477936/126‑1 (MQ=255)                                                     gagaGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGc                                                                                   >  2:92260/1‑140 (MQ=255)                                                     gagaGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGc                                                                                   <  1:347411/140‑1 (MQ=255)                                                          ccTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTaa                                                                              >  1:191760/1‑140 (MQ=255)                                                          ccTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTaa                                                                              <  1:222410/140‑1 (MQ=255)                                                               cTTGAGCTAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCtt                                                                         >  1:214356/1‑140 (MQ=255)                                                                   aGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTG‑CCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGAc                                                                    <  2:262005/140‑1 (MQ=255)                                                                              cATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGt                                                          >  2:571644/1‑140 (MQ=255)                                                                                  gAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCa                                                      >  1:677617/1‑140 (MQ=255)                                                                                   aaGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCaa                                                     >  1:133185/1‑140 (MQ=255)                                                                                   aaGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCaa                                                     >  1:237743/1‑140 (MQ=255)                                                                                   aaGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCaa                                                     <  2:374548/140‑1 (MQ=255)                                                                                    aGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCaaa                                                    >  2:72477/1‑140 (MQ=255)                                                                                    aGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCaaa                                                    <  1:302660/140‑1 (MQ=255)                                                                                        aaaGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGc                                                <  1:522163/140‑1 (MQ=255)                                                                                        aaaGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGc                                                <  2:191760/140‑1 (MQ=255)                                                                                         aagaagTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGcc                                               <  1:707378/140‑1 (MQ=255)                                                                                         aagaagTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGcc                                               <  1:92260/140‑1 (MQ=255)                                                                                         aagaagTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGcc                                               <  1:360951/140‑1 (MQ=255)                                                                                              gTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCa                                          <  1:571644/140‑1 (MQ=255)                                                                                                        cAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATc                                <  2:677617/140‑1 (MQ=255)                                                                                                          aaGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCa                              <  2:623008/140‑1 (MQ=255)                                                                                                                      ggCCGCCGCCTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTgg                  >  1:243830/1‑140 (MQ=255)                                                                                                                       gccgccgccTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAg                      >  1:464054/1‑135 (MQ=255)                                                                                                                       gccgccgccTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAg                      <  2:464054/135‑1 (MQ=255)                                                                                                                       gccgccgccTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTggg                 >  2:575446/1‑140 (MQ=255)                                                                                                                       gccgccgccTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTggg                 >  2:108846/1‑140 (MQ=255)                                                                                                                       gccgccgccTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTggg                 >  2:149239/1‑140 (MQ=255)                                                                                                                           ccgccTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGACCTGGCATGAACCTATCCATAATTTAGGTGGGATGc             >  1:639371/1‑140 (MQ=255)                                                                                                                            cgccTTCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGcc            <  2:250433/140‑1 (MQ=255)                                                                                                                                 tCTGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTg       >  2:602780/1‑140 (MQ=255)                                                                                                                                   tGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCg     <  2:133185/140‑1 (MQ=255)                                                                                                                                   tGGAGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCg     <  2:237743/140‑1 (MQ=255)                                                                                                                                      aGGCATTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTgg  <  2:214356/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        AACCTGGTACTGTTGTCCGTTTTAGCATCGGGCAGGAAAAGCCTGTAACCTGGAGAGCCTTTCTTGAGCCAGGAATACATTGAAGACAAAGAAGTCACATTGACAAAGTTAAGTAGCGGCCGCCGCCTTCTGGAGGCGTTGCTGATCCTTATTGTCCTGTTTGCCGTCTGGTTGATGGCTGCCTTACTAAGCTTTAACCCTTCGGACCCCAGCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGG  >  NC_000913/933161‑933433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑