| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,604,344 | 0 | C | T | 50.0% | 22.1 / 38.3 | 42 | S62F (TCT→TTT) | bglJ | DNA‑binding transcriptional regulator BglJ |
| Reads supporting (aligned to +/- strand): ref base C (1/20); new base T (21/0); total (22/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.17e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGAT > NC_000913/4604207‑4604464 | aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttttt > 1:213568/1‑140 (MQ=255)aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctt > 1:224322/1‑140 (MQ=255) gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttttttt > 2:393287/1‑140 (MQ=255) tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTttttctttttct < 2:427216/140‑1 (MQ=255) tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTttttctttttct < 1:225337/140‑1 (MQ=255) aTGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑c < 1:393287/140‑1 (MQ=255) taTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTT‑CCCTGAGaa > 2:225337/1‑140 (MQ=255) gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAtttttttttttt > 2:144019/1‑123 (MQ=255) gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttcttttt < 1:144019/123‑1 (MQ=255) tATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAgc < 1:425407/140‑1 (MQ=255) gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTG‑CCATGAGAAGGGAGCGCag > 1:166985/1‑140 (MQ=255) gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCag > 1:177448/1‑140 (MQ=255) agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCaga < 2:224322/140‑1 (MQ=255) gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCagag < 1:237115/140‑1 (MQ=255) gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCagag < 2:394883/140‑1 (MQ=255) agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTT‑CCATGAGAAGTGAGCGCagagg > 1:83994/1‑139 (MQ=255) tttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCTTTTTTTTTTTTTTTT‑CCATGAGAAGGGAGGGCagggggg > 1:113548/1‑135 (MQ=255) ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAggg < 1:482580/140‑1 (MQ=255) ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAggg < 1:332787/140‑1 (MQ=255) tATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGa < 1:257228/140‑1 (MQ=255) aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGAt > 1:101825/1‑140 (MQ=255) tAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAc < 2:468163/140‑1 (MQ=255) tAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAc < 2:157674/140‑1 (MQ=255) ataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGAtt < 2:177448/140‑1 (MQ=255) gCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATT‑AAGt < 2:488520/140‑1 (MQ=255) tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTG‑CCCTGTGCAGTGAGGGCGGAGGGGGGTTCTCTTGCCTTTCTGAACTGGGGGTT‑atactt > 2:168381/1‑135 (MQ=255) ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTG‑CCCTGAGAAGGGAGCGCAGAGAGGGGTTTTTTTGCCTGACTGAACTGGGGATT‑TAGtttt > 2:384288/1‑139 (MQ=255) aTACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATT‑AAGTTTCcg < 2:358808/140‑1 (MQ=255) ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATT‑AAGTTTCCGCGTAcc < 1:168381/140‑1 (MQ=255) ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATT‑AAGTTTCCGCGTAcc < 1:305104/140‑1 (MQ=255) taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTT‑GCATGAGAAGTGAGCGCGGAGGGGGATTTTCTTGCCTGTGTGAAAGTGCGGTT‑TAGATTTCGCGTGccc > 2:275363/1‑140 (MQ=255) taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTT‑CCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGGGAGT‑AAGTTTTCGCGGAccc > 2:389407/1‑140 (MQ=255) taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTT‑CCATGAGAAGTGAGGGCAGAGAGGGGTTATCTTTCCTGGCTGAACTGGCGATT‑AAGTTTTCGCGTAccc > 2:419927/1‑140 (MQ=255) taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTG‑CCATGAGAAGTGAGGGCAGAGAGGGGTTATCTTTCCTGACTGAACTGGCGGTT‑AAGGTTTCGCGTAccc > 2:216629/1‑140 (MQ=255) gTCAGTAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTT‑CCATGAGGAGTGAGGGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATT‑AAGGTTTCGCGGAACCGGCgg > 2:104258/1‑139 (MQ=255) gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTG‑CCATGAGAAGGGGGCGCAGAGAGGGAATTTCTTTCCTTACTGAACCCGCGATT‑AAGTTTTCGCGCACCCGGCgg > 2:34335/1‑139 (MQ=255) taaGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTG‑CCATGAGAAGAGAGGGGCGAGAGGGGGTATATTTCCTGACTGAACTGGGGGTT‑AAGGTTTCCCGGACCCGGCGtt > 2:438250/3‑140 (MQ=255) tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTTATTTTTTTTTTTTTTGCCCATG‑GGAGTGAGCGCGGAGAGGGGTTATATTGCCTGACTTAACTGGCGGTT‑TAGTTTTCGCGGTCCCcgcggt > 2:254791/1‑138 (MQ=255) tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTG‑CCCTGAGAAGGGAGGGCGGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATT‑AAGATTTCGCGTACCCCGCGtt > 2:430341/1‑140 (MQ=255) cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTG‑CCATGAGAAGGGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGGTT‑AAGTTTTCGCGGACCCGGCGttt > 2:305104/1‑140 (MQ=255) gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTT‑CCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATT‑AAGTTTTCGCGTACCCCGCGGTTTGt > 2:157078/1‑140 (MQ=255) gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTG‑CCCTTAGAAGGGGGCGCAGAGAGGGGTTATTTTGTCTGACTGAACTGGGGGTT‑TAATTTTCGCGGACCCGGCGGTTTGt > 2:368735/1‑140 (MQ=255) tCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTT‑CCCTGAGGAGGGAGCGCAGAGAGGGGTTATCTTTCCTGACTGAACTGGCGGTT‑AAGTTTTCGCGTACCCGGCGGTTTGtttt > 2:416297/1‑138 (MQ=255) tCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTAt < 1:34335/140‑1 (MQ=255) tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTT‑CCATGAGGAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTTAACTGGCGATT‑TAGTTTTCGCGTACCCCGGGGTTTGTTTTTgt > 2:272183/1‑139 (MQ=255) gagacagCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGga < 1:54930/134‑1 (MQ=255) cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGga < 1:416297/140‑1 (MQ=255) gATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTT‑CCCTGAGAAGGGAGGGCGGAGAGGGGGTTTTTTGCCTGGCTGGAATGGCGATTAAAGTTTCC‑CGTTCCCCGCGGTTTGTTTTTTCGggtgg > 2:76325/1‑139 (MQ=255) aTCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTT‑CCCTGAGAAGTGAGCGCAGAGAGGGATTATTTTTCCTGACTGAACTGGCGGTT‑AAGTTTTCGCGTACCCCGCGTTTTGTTATTGCGGGTGATGATATTgctgtctc > 2:54930/1‑133 (MQ=255) tctcGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCgg < 2:83994/140‑1 (MQ=255) tttGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGAt < 2:101825/140‑1 (MQ=255) | ATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTG‑CCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATT‑AAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGAT > NC_000913/4604207‑4604464 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |