Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,327,395	G→A	I90I (ATC→ATT)	rlmE ←	23S rRNA 2'‑O‑ribose U2552 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,327,395	0	G	A	100.0%	92.5 / NA	27	I90I (ATC→ATT)	rlmE	23S rRNA 2'‑O‑ribose U2552 methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/16);  total (11/16)

CGGTGTTCCGCTCATGTTTGGTGCCATATCGGACATGACAACCTGGACTTTGCTGTCGCCAACGCGCTCCAGCAGTGCTTTCATCACCAGTTCATCACGAAAATCGCCCTGAAGAAAGTCCACACCAACGATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACATATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACCGTCATTCCC  >  NC_000913/3327266‑3327512                                                                                                                                  |                                                                                                                      cgGTGTTCCGCTCATGTTTGGTGCCATATCGGACATGACAACCTGGACTTTGCTGTCGCCAACGCGCTCCAGCAGTGCTTTCATCACCAGTTCATCACGAAAATCGCCCTGAAGAAAGTCCACACCAACAATAGGATCCa                                                                                                             <  1:296456/140‑1 (MQ=255) cgGTGTTCCGCTCATGTTTGGTGCCATATCGGACATGACAACCTGGACTTTGCTGTCGCCAACGCGCTCCAGCAGTGCTTTCATCACCAGTTCATCACGAAAATCGCCCTGAAGAAAGTCCACACCAACAATAGGATCCa                                                                                                             <  2:276507/140‑1 (MQ=255)  ggTGTTCCGCTCATGTTTGGTGCCATATCGGACATGACAACCTGGACTTTGCTGTCGCCAACGCGCTCCAGCAGTGCTTTCATCACCAGTTCATCACGAAAATCGCCCTGAAGAAAGTCCACACCAACAATAGGATCCAt                                                                                                            <  1:102989/140‑1 (MQ=255)  ggTGTTCCGCTCATGTTTGGTGCCATATCGGACATGACAACCTGGACTTTGCTGTCGCCAACGCGCTCCAGCAGTGCTTTCATCACCAGTTCATCACGAAAATCGCCCTGAAGAAAGTCCACACCAACAATAGGATCCAt                                                                                                            <  2:43048/140‑1 (MQ=255)  ggTGTTCCGCTCATGTTTGGTGCCATATCGGACATGACAACCTGGACTTTGCTGTCGCCAACGCGCTCCAGCAGTGCTTTCATCACCAGTTCATCACGAAAATCGCCCTGAAGAAAGTCCACACCAACAATAGGATCCAt                                                                                                            <  1:334344/140‑1 (MQ=255)   gtgtTCCGCTCATGTTTGGTGCCATATCGGACATGACAACCTGGACTTTGCTGTCGCCAACGCGCTCCAGCAGTGCTTTCATCACCAGTTCATCACGAAAATCGCCCTGAAGAAAGTCCACACCAACAATAGGATCCATa                                                                                                           >  1:492668/1‑140 (MQ=255)                gTTTGGTGCCATATCGGACATGACAACCTGGACTTTGCTGTCGCCAACGCGCTCCAGCAGTGCTTTCATCACCAGTTCATCACGAAAATCGCCCTGAAGAAAGTCCACACCAACAATAGGATCCATAGGTAAAAGATCGc                                                                                              >  2:251674/1‑140 (MQ=255)                     gTGCCATATCGGACATGACAACCTGGACTTTGCTGTCGCCAACGCGCTCCAGCAGTGCTTTCATCACCAGTTCATCACGAAAATCGCCCTGAAGAAAGTCCACACCAACAATAGGATCCATAGGTAAAAGATCGCAAGCg                                                                                         >  1:177276/1‑140 (MQ=255)                                       cAACCTGGACTTTGCTGTCGCCAACGCGCTCCAGCAGTGCTTTCATCACCAGTTCATCACGAAAATCGCCCTGAAGAAAGTCCACACCAACAATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTgccg                                                                       >  1:398801/1‑140 (MQ=255)                                                 tttGCTGTCGCCAACGCGCTCCAGCAGTGCTTTCATCACCAGTTCATCACGAAAATCGCCCTGAAGAAAGTCCACACCAACAATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTggg                                                             <  2:171021/140‑1 (MQ=255)                                                 tttGCTGTCGCCAACGCGCTCCAGCAGTGCTTTCATCACCAGTTCATCACGAAAATCGCCCTGAAGAAAGTCCACACCAACAATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTggg                                                             <  2:305594/140‑1 (MQ=255)                                                  ttGCTGTCGCCAACGCGCTCCAGCAGTGCTTTCATCACCAGTTCATCACGAAAATCGCCCTGAAGAAAGTCCACACCAACAATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAAt                                                                  >  1:353945/1‑134 (MQ=255)                                                  ttGCTGTCGCCAACGCGCTCCAGCAGTGCTTTCATCACCAGTTCATCACGAAAATCGCCCTGAAGAAAGTCCACACCAACAATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAAt                                                                  <  2:353945/134‑1 (MQ=255)                                                   tGCTGTCGCCAACGCGCTCCAGCAGTGCTTTCATCACCAGTTCATCACGAAAATCGCCCTGAAGAAAGTCCACACCAACAATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTg                                                           <  2:398801/140‑1 (MQ=255)                                                   tGCTGTCGCCAACGCGCTCCAGCAGTGCTTTCATCACCAGTTCATCACGAAAATCGCCCTGAAGAAAGTCCACACCAACAATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTg                                                           <  1:251674/140‑1 (MQ=255)                                                   tGCTGTCGCCAACGCGCTCCAGCAGTGCTTTCATCACCAGTTCATCACGAAAATCGCCCTGAAGAAAGTCCACACCAACAATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTg                                                           >  1:137043/1‑140 (MQ=255)                                                     cTGTCGCCAACGCGCTCCAGCAGTGCTTTCATCACCAGTTCATCACGAAAATCGCCCTGAAGAAAGTCCACACCAACAATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAAtt                                                                 <  2:91806/132‑1 (MQ=255)                                                     cTGTCGCCAACGCGCTCCAGCAGTGCTTTCATCACCAGTTCATCACGAAAATCGCCCTGAAGAAAGTCCACACCAACAATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAAtt                                                                 >  1:91806/1‑132 (MQ=255)                                                                     ccAGCAGTGCTTTCATCACCAGTTCATCACGAAAATCGCCCTGAAGAAAGTCCACACCAACAATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACATATTGTGaccaa                                         <  1:191832/140‑1 (MQ=255)                                                                         cagTGCTTTCATCACCAGTTCATCACGAAAATCGCCCTGAAGAAAGTCCACACCAACAATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACATATTGTGACCAaccac                                     >  2:267816/1‑140 (MQ=255)                                                                          agTGCTTTCATCACCAGTTCATCACGAAAATCGCCCTGAAGAAAGTCCACACCAACAATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACATATTGTGACCAaccacc                                    <  2:492668/140‑1 (MQ=255)                                                                                   atcaCCAGTTCATCACGAAAATCGCCCTGAAGAAAGTCCACACCAACAATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACATATTGTGACCAACCACCCGGagcagc                           >  2:125105/1‑140 (MQ=255)                                                                                       ccAGTTCATCACGAAAATCGCCCTGAAGAAAGTCCACACCAACAATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACATATTGTGACCAACCACCCGGAGCAGCACCa                       <  2:265095/140‑1 (MQ=255)                                                                                             catcaCGAAAATCGCCCTGAAGAAAGTCCACACCAACAATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACATATTGTGACCAACCACCCGGAGCAGCACCAAGGTCg                 >  1:270363/1‑140 (MQ=255)                                                                                                    aaaaTCGCCCTGAAGAAAGTCCACACCAACAATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACATATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACCg          >  2:5356/1‑140 (MQ=255)                                                                                                            ccTGAAGAAAGTCCACACCAACAATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACATATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACCGTCATTccc  <  2:137043/140‑1 (MQ=255)                                                                                                            ccTGAAGAAAGTCCACACCAACAATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACATATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACCGTCATTccc  <  2:101118/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                      CGGTGTTCCGCTCATGTTTGGTGCCATATCGGACATGACAACCTGGACTTTGCTGTCGCCAACGCGCTCCAGCAGTGCTTTCATCACCAGTTCATCACGAAAATCGCCCTGAAGAAAGTCCACACCAACGATAGGATCCATAGGTAAAAGATCGCAAGCGATGATGCGGCCTTTGCCGCCAATTTGGGTGACCACATATTGTGACCAACCACCCGGAGCAGCACCAAGGTCGACAACCGTCATTCCC  >  NC_000913/3327266‑3327512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑