Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,585,592	C→G	D299H (GAT→CAT)	ydeP ←	putative oxidoreductase YdeP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,585,592	0	C	G	100.0%	103.1 / NA	27	D299H (GAT→CAT)	ydeP	putative oxidoreductase YdeP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (11/16);  total (11/16)

CAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCAGAGTTCGTCAGCATTTCA  >  NC_000913/1585463‑1585704                                                                                                                                  |                                                                                                                 cAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATGATCGCGCTCa                                                                                                        <  1:462314/140‑1 (MQ=255)               atatCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATGATCGCGCTCAATTAACAGGCGcat                                                                                          >  2:7755/1‑140 (MQ=255)                 atCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATGATCGCGCTCAATTAACAGGCGcatca                                                                                        <  2:158112/140‑1 (MQ=255)                      tCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATGATCGCGCTCAATTAACAGGCGca                                                                                           >  1:216970/1‑132 (MQ=255)                      tCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATGATCGCGCTCAATTAACAGGCGca                                                                                           <  2:216970/132‑1 (MQ=255)                       ccACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATGATCGCGCTCAATTAACAGGCGCATCATCCCCt                                                                                  <  2:290681/140‑1 (MQ=255)                          cTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATGATCGCGCTCAATTAACAGGCGCATCATCCCCTTGa                                                                               >  1:261676/1‑140 (MQ=255)                             ggAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATGATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCa                                                                            >  1:117530/1‑140 (MQ=255)                                         gTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATGATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGc                                                                >  1:480031/1‑140 (MQ=255)                                                   aGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATGATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATgcg                                                      <  1:212572/140‑1 (MQ=255)                                                   aGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATGATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATgcg                                                      <  2:58829/140‑1 (MQ=255)                                                   aGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATGATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATgcg                                                      <  2:400584/140‑1 (MQ=255)                                                   aGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATGATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATgc                                                       >  1:184224/1‑139 (MQ=255)                                                   aGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATGATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATgc                                                       <  2:184224/139‑1 (MQ=255)                                                     cTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATGATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATgc                                                       >  2:237943/1‑137 (MQ=255)                                                     cTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATGATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATgc                                                       <  1:237943/137‑1 (MQ=255)                                                     cTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATGATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCa                                                    <  2:98937/140‑1 (MQ=255)                                                          cAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATGATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTta                                               <  2:117530/140‑1 (MQ=255)                                                                   cGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATGATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCa                                      <  2:261676/140‑1 (MQ=255)                                                                              tGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATGATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACt                            >  2:345271/1‑139 (MQ=255)                                                                              tGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATGATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACt                            <  1:345271/139‑1 (MQ=255)                                                                                aaTAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATGATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCa                                      >  1:464508/1‑127 (MQ=255)                                                                                aaTAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATGATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCa                                      <  2:464508/127‑1 (MQ=255)                                                                                aaTAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATGATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAg                         >  1:442107/1‑140 (MQ=255)                                                                                     aCTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATGATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCa                    <  2:442107/140‑1 (MQ=255)                                                                                              caAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATGATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCAGAGTTCGTc           <  2:480031/140‑1 (MQ=255)                                                                                                       aaGGCCGACCTGCGGCGCTTGCAGCATGATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCAGAGTTCGTCAGCATTTCa  >  2:394724/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                 CAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTCGTCAAAGCCGACGGTATGCGTTTGAATAAACTCGTCATCAAGCAATGAAGGCCGACCTGCGGCGCTTGCAGCATCATCGCGCTCAATTAACAGGCGCATCATCCCCTTGAGCAACGCCATATCGCCACCAATGCGCACGTTATAGTAGGCACTGGCCAACTGAGTCTCAGAGTTCGTCAGCATTTCA  >  NC_000913/1585463‑1585704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑