Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,427,482	(A)7→6	intergenic (‑60/‑212)	yjfZ ← / → ytfA	protein YjfZ/putative transcriptional regulator YtfA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,427,476	0	A	.	100.0%	263.3 / NA	61	intergenic (‑54/‑218)	yjfZ/ytfA	protein YjfZ/putative transcriptional regulator YtfA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (27/34);  total (27/34)

TTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAGAAAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCATTATGGATATAACGATGCACCCGGGGAGAAAATGACTTCCACGCCT  >  NC_000913/4427348‑4427610                                                                                                                                 |                                                                                                                                       ttttaCTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGata                                                                                                                            <  1:452902/140‑1 (MQ=255)              tCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATaa                                                                                                               >  1:374818/1‑140 (MQ=255)              tCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATaa                                                                                                               >  2:443501/1‑140 (MQ=255)              tCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATaa                                                                                                               >  1:102522/1‑140 (MQ=255)              tCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATaa                                                                                                               >  1:222227/1‑140 (MQ=255)                        aaaaaCGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTATGTTTTc                                                                                                     >  1:180882/1‑140 (MQ=255)                          aaaCGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTg                                                                                                   <  2:222227/140‑1 (MQ=255)                          aaaCGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTg                                                                                                   <  1:321103/140‑1 (MQ=255)                          aaaCGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTg                                                                                                   <  1:323144/140‑1 (MQ=255)                            aCGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGaa                                                                                                 <  1:243464/140‑1 (MQ=255)                            aCGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGaa                                                                                                 <  1:182282/140‑1 (MQ=255)                                gaTAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAgtgg                                                                                             >  1:462956/1‑140 (MQ=255)                                          cAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGc                                                                                   >  1:214683/1‑140 (MQ=255)                                          cAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGc                                                                                   >  1:557764/1‑140 (MQ=255)                                           aGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCg                                                                                  <  1:358694/140‑1 (MQ=255)                                             gAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGa                                                                                <  2:365537/140‑1 (MQ=255)                                             gAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGa                                                                                <  2:80913/140‑1 (MQ=255)                                             gAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGa                                                                                <  1:443501/140‑1 (MQ=255)                                                 tGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGt                                                                            <  2:214683/140‑1 (MQ=255)                                                  gAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTg                                                                           <  2:51060/140‑1 (MQ=255)                                                   aataaataGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGa                                                                          <  1:647564/140‑1 (MQ=255)                                                    ataaataGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGaa                                                                         <  2:462956/140‑1 (MQ=255)                                                           gTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATAtt                                                                  >  1:23330/1‑140 (MQ=255)                                                                 ggTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTa                                                                 >  1:663980/1‑135 (MQ=255)                                                                 ggTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTa                                                                 <  2:663980/135‑1 (MQ=255)                                                                   tAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCaa                                                          >  1:568086/1‑140 (MQ=255)                                                                      tGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCaaaaa                                                       >  2:257576/1‑140 (MQ=255)                                                                         cATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCg                                                    <  2:284882/140‑1 (MQ=255)                                                                         cATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCg                                                    <  2:276966/140‑1 (MQ=255)                                                                         cATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTATGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCg                                                    <  2:180882/140‑1 (MQ=255)                                                                          aTTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGc                                                   >  2:381975/1‑140 (MQ=255)                                                                          aTTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGc                                                   >  2:443710/1‑140 (MQ=255)                                                                          aTTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGc                                                   >  2:280218/1‑140 (MQ=255)                                                                            tGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGc                                                   >  1:333022/1‑138 (MQ=255)                                                                            tGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGc                                                   <  2:333022/138‑1 (MQ=255)                                                                             gTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCa                                                >  1:399042/1‑140 (MQ=255)                                                                               aaTGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCAtt                                              <  2:151676/140‑1 (MQ=255)                                                                               aaTGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCAtt                                              <  2:605011/140‑1 (MQ=255)                                                                                 tGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCATTAt                                            <  2:436708/140‑1 (MQ=255)                                                                                 tGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCATTAt                                            <  1:193886/140‑1 (MQ=255)                                                                                 tGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCATTAt                                            <  1:443710/140‑1 (MQ=255)                                                                                     aCCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCATTATGGat                                        >  1:462091/1‑140 (MQ=255)                                                                                     aCCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCATTATGGat                                        >  1:512854/1‑140 (MQ=255)                                                                                      ccTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCATTATGGata                                       >  1:276596/1‑140 (MQ=255)                                                                                      ccTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCATTATGGata                                       <  2:376594/140‑1 (MQ=255)                                                                                      ccTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCATTATGGata                                       >  1:495911/1‑140 (MQ=255)                                                                                          tttAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCATTATGGATATAAc                                   >  2:648018/1‑140 (MQ=255)                                                                                           ttAATGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCATTATGGATATAACg                                  >  1:526715/1‑140 (MQ=255)                                                                                            tAATGTTTTTATAAATATCGCAGCTGAATGTTCATAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCATTATGGATATAACGa                                 >  2:661038/1‑140 (MQ=255)                                                                                              aTGTTTTTATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCATTATGGATATAACGATg                               <  1:254336/140‑1 (MQ=255)                                                                                                    ttATAAATATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCATTATGGATATAACGATGCACCCg                         >  2:521494/1‑140 (MQ=255)                                                                                                         aaTATCGCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCATTATGGATATAACGATGCACCCGGGgag                    >  1:646506/1‑140 (MQ=255)                                                                                                               gCAGCTGAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCATTATGGATATAACGATGCACCCGGGGAGAAAATg              <  1:521494/140‑1 (MQ=255)                                                                                                                     gAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCATTATGGATATAACGATGCACCCGGGGAGAAAATGACTTcc        <  2:345196/140‑1 (MQ=255)                                                                                                                     gAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCATTATGGATATAACGATGCACCCGGGGAGAAAATGACTTcc        <  2:526715/140‑1 (MQ=255)                                                                                                                     gAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCATTATGGATATAACGATGCACCCGGGGAGAAAATGACTTcc        <  2:276596/140‑1 (MQ=255)                                                                                                                     gAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCATTATGGATATAACGATGCACCCGGGGAGAAAATGACTTcc        <  1:58927/140‑1 (MQ=255)                                                                                                                     gAATGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCATTATGGATATAACGATGCACCCGGGGAGAAAATGACTTcc        <  2:695298/140‑1 (MQ=255)                                                                                                                      aaTGTTCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCATTATGGATATAACGATGCACCCGGGGAGAAAATGACTTCCa       <  1:257576/140‑1 (MQ=255)                                                                                                                          ttCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCATTATGGATATAACGATGCACCCGGGGAGAAAATGACTTCCACGcc   <  2:91073/140‑1 (MQ=255)                                                                                                                           tCACAG‑AAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCATTATGGATATAACGATGCACCCGGGGAGAAAATGACTTCCACGCCt  >  1:320104/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                       TTTTACTGGTTTGTCGGTGGACGAAAAACGAGATAAATATGCAGGAAATGAATAAATAGTCGTTGGTAATGTCATTGTAATGTTACCTGTTTAATGTTTTTATAAATATCGCAGCTGAATGTTCACAGAAAAAAATCGATATTAATAAAAATAAGTTTGTTTTCTGAAGTGGTGAAAATAGCGGATGGTGAAAGATATTAATATCAAAAATCGCTCATTATGGATATAACGATGCACCCGGGGAGAAAATGACTTCCACGCCT  >  NC_000913/4427348‑4427610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑