Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,195,676	Δ1 bp	coding (554/1251 nt)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,195,676	0	T	.	100.0%	125.1 / NA	30	coding (554/1251 nt)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (14/16);  total (14/16)

GTTAAACACCCTGAACTGACCGATATGGTTATCTTCCGTGAAAACTCGGAAGACATTTATGCGGGTATCGAATGGAAAGCAGACTCTGCCGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATACGCAATTGCTAACGATCGTGA  >  NC_000913/1195543‑1195790                                                                                                                                      |                                                                                                                   gTTAAACACCCTGAACTGACCGATATGGTTATCTTCCGTGAAAACTCGGAAGACATTTATGCGGGTATCGAATGGAAAGCAGACTCTGCCGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGaaa                                                                                                             >  2:45744/1‑140 (MQ=255) gTTAAACACCCTGAACTGACCGATATGGTTATCTTCCGTGAAAACTCGGAAGACATTTATGCGGGTATCGAATGGAAAGCAGACTCTGCCGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGaaa                                                                                                             >  2:125890/1‑140 (MQ=255) gTTAAACACCCTGAACTGACCGATATGGTTATCTTCCGTGAAAACTCGGAAGACATTTATGCGGGTATCGAATGGAAAGCAGACTCTGCCGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGaaa                                                                                                             >  2:113741/1‑140 (MQ=255)                   aCCGATATGGTTATCTTCCGTGAAAACTCGGAAGACATTTATGCGGGTATCGAATGGAAAGCAGACTCTGCCGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGAAAATTCGCTTCCCGGAACAt                                                                                           >  2:581555/1‑140 (MQ=255)                   aCCGATATGGTTATCTTCCGTGAAAACTCGGAAGACATTTATGCGGGTATCGAATGGAAAGCAGACTCTGCCGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGAAAATTCGCTTCCCGGAACAt                                                                                           <  1:132505/140‑1 (MQ=255)                        tatGGTTATCTTCCGTGAAAACTCGGAAGACATTTATGCGGGTATCGAATGGAAAGCAGACTCTGCCGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGAAAATTCGCTTCCCGGAACATTGTgg                                                                                      >  2:547961/1‑140 (MQ=255)                           ggTTATCTTCCGTGAAAACTCGGAAGACATTTATGCGGGTATCGAATGGAAAGCAGACTCTGCCGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGAAAATTCGCTTCCCGGAACATTGTGGTAt                                                                                   >  2:654119/1‑140 (MQ=255)                           ggTTATCTTCCGTGAAAACTCGGAAGACATTTATGCGGGTATCGAATGGAAAGCAGACTCTGCCGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGAAAATTCGCTTCCCGGAACATTGTGGTAt                                                                                   <  2:289895/140‑1 (MQ=255)                           ggTTATCTTCCGTGAAAACTCGGAAGACATTTATGCGGGTATCGAATGGAAAGCAGACTCTGCCGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGAAAATTCGCTTCCCGGAACATTGTGGTAt                                                                                   <  1:547961/140‑1 (MQ=255)                           ggTTATCTTCCGTGAAAACTCGGAAGACATTTATGCGGGTATCGAATGGAAAGCAGACTCTGCCGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGAAAATTCGCTTCCCGGAACATTGTGGTAt                                                                                   <  1:27849/140‑1 (MQ=255)                             ttATCTTCCGTGAAAACTCGGAAGACATTTATGCGGGTATCGAATGGAAAGCAGACTCTGCCGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGAAAATTCGCTTCCCGGAACATTGTGGTATCg                                                                                 >  2:449593/1‑140 (MQ=255)                               aTCTTCCGTGAAAACTCGGAAGACATTTATGCGGGTATCGAATGGAAAGCAGACTCTGCCGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGt                                                                               >  1:325980/1‑140 (MQ=255)                                      gTGAAAACTCGGAAGACATTTATGCGGGTATCGAATGGAAAGCAGACTCTGCCGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGAAAATTCGCTTCCCGGAACATtgt                                                                                        <  1:181/124‑1 (MQ=255)                                      gTGAAAACTCGGAAGACATTTATGCGGGTATCGAATGGAAAGCAGACTCTGCCGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGAAAATTCGCTTCCCGGAACATtgt                                                                                        >  2:181/1‑124 (MQ=255)                                      gTGAAAACTCGGAAGACATTTATGCGGGTATCGAATGGAAAGCAGACTCTGCCGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGc                                                                        >  2:291899/1‑140 (MQ=255)                                       tGAAAACTCGGAAGACATTTATGCGGGTATCGAATGGAAAGCAGACTCTGCCGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGcc                                                                       <  1:449593/140‑1 (MQ=255)                                                           aTGCGGGTATCGAATGGAAAGCAGACTCTGCCGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCa                                                   <  1:47295/140‑1 (MQ=255)                                                           aTGCGGGTATCGAATGGAAAGCAGACTCTGCCGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTAGGAAGAAGGCACCa                                                   <  1:45744/140‑1 (MQ=255)                                                               gggTATCGAATGGAAAGCAGACTCTGCCGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCa                                                   >  2:171763/1‑136 (MQ=255)                                                               gggTATCGAATGGAAAGCAGACTCTGCCGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCa                                                   <  1:171763/136‑1 (MQ=255)                                                                                  gACTCTGCCGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATc                            >  2:133028/1‑140 (MQ=255)                                                                                      ctGCCGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAAt                        >  2:269891/1‑140 (MQ=255)                                                                                            aCGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATACGCaa                  <  1:291899/140‑1 (MQ=255)                                                                                            aCGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATACGCaa                  <  1:540028/140‑1 (MQ=255)                                                                                             cGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATACGCAAt                 >  2:163245/1‑140 (MQ=255)                                                                                              gCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATACGCAAtt                <  1:113741/140‑1 (MQ=255)                                                                                                  agaAAGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATACGCAATTGCTa            <  1:654119/140‑1 (MQ=255)                                                                                                    aaaGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCATACGTCTGGTTCGTGCAGCGATCGAATACGCAATTGCTAAc          <  1:125890/140‑1 (MQ=255)                                                                                                    aaaGTGATTAAATTCCTGCGTGAAGAGATGGGGG‑GAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATACGCAATTGCTAAc          <  1:163245/140‑1 (MQ=255)                                                                                                            tAAATTCCTGCGTGAAGAGATGGGGG‑GAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATACGCAATTGCTAACGATCgtga  <  2:325980/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                   GTTAAACACCCTGAACTGACCGATATGGTTATCTTCCGTGAAAACTCGGAAGACATTTATGCGGGTATCGAATGGAAAGCAGACTCTGCCGACGCCGAGAAAGTGATTAAATTCCTGCGTGAAGAGATGGGGGTGAAGAAAATTCGCTTCCCGGAACATTGTGGTATCGGTATTAAGCCGTGTTCGGAAGAAGGCACCAAACGTCTGGTTCGTGCAGCGATCGAATACGCAATTGCTAACGATCGTGA  >  NC_000913/1195543‑1195790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑