Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	954,589	A→G	intergenic (‑123/+283)	focA ← / ← ycaO	formate channel FocA/ribosomal protein S12 methylthiotransferase accessory factor YcaO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	954,589	0	A	G	100.0%	130.5 / NA	34	intergenic (‑123/+283)	focA/ycaO	formate channel FocA/ribosomal protein S12 methylthiotransferase accessory factor YcaO
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/14);  total (20/14)

TTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATAT  >  NC_000913/954453‑954717                                                                                                                                         |                                                                                                                                 ttGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATGCgg                                                                                                                               >  1:249956/1‑140 (MQ=255)                   aCTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATc                                                                                                             >  1:567907/1‑140 (MQ=255)                       tctctTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCaaa                                                                                                         >  2:442955/1‑140 (MQ=255)                         tctTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCaaata                                                                                                       >  1:187456/1‑140 (MQ=255)                                   gTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAAtt                                                                                             >  1:257789/1‑140 (MQ=255)                                     cGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAAtttt                                                                                           >  2:198892/1‑140 (MQ=255)                                       gCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTaa                                                                                         <  2:249956/140‑1 (MQ=255)                                         gacgaGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTaaaa                                                                                       <  2:421015/140‑1 (MQ=255)                                                         aGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTg                                                                       >  1:239306/1‑140 (MQ=255)                                                               ataGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAAtttt                                                                 >  1:270741/1‑140 (MQ=255)                                                                 aGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAAtttttt                                                               >  2:445908/1‑140 (MQ=255)                                                                     gagaAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTTTTTCCGc                                                           >  1:373456/1‑140 (MQ=255)                                                                      agaAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTTTTTCCGCa                                                          >  1:702392/1‑140 (MQ=255)                                                                        aaaTTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTTTTTCCGCAtc                                                        >  2:187452/1‑140 (MQ=255)                                                                         aaTTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTTTTTCCGCAtcc                                                       <  2:567907/140‑1 (MQ=255)                                                                         aaTTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTTTTTCCGCAtcc                                                       <  1:198892/140‑1 (MQ=255)                                                                           ttGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTTTTTCCGCAtcctc                                                     <  1:445908/140‑1 (MQ=255)                                                                                  aGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTTTTTCCGCATCCTCCGCTaaa                                              <  2:187456/140‑1 (MQ=255)                                                                                        tatCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGtt                                        <  2:641807/140‑1 (MQ=255)                                                                                           ctcGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGttaat                                     <  2:702392/140‑1 (MQ=255)                                                                                             cGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGttaatta                                   >  2:258768/1‑140 (MQ=255)                                                                                             cGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGttaatta                                   >  1:605741/1‑140 (MQ=255)                                                                                              gCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGttaattaa                                  >  2:274593/1‑140 (MQ=255)                                                                                               ccTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTaaa                                 >  1:120078/1‑140 (MQ=255)                                                                                                   gCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAggg                             >  2:399592/1‑140 (MQ=255)                                                                                                        taGGCCCGTAACTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCAt                        >  2:116550/1‑140 (MQ=255)                                                                                                              cGTAACTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCg                  >  1:643786/1‑140 (MQ=255)                                                                                                                 aaCTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAAt               >  2:302076/1‑140 (MQ=255)                                                                                                                   cTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATaa             <  2:215388/140‑1 (MQ=255)                                                                                                                   cTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATaa             <  2:239306/140‑1 (MQ=255)                                                                                                                   cTCGCATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATaa             <  2:643786/140‑1 (MQ=255)                                                                                                                       cATGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTa         <  1:442955/140‑1 (MQ=255)                                                                                                                        aTGGTTTTTATGCAAATGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTaa        <  1:187452/140‑1 (MQ=255)                                                                                                                                      aaTGCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAatat  <  1:368076/132‑1 (MQ=255)                                                                                                                                         |                                                                                                                                 TTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATAT  >  NC_000913/954453‑954717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑