Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,397,754	T→A	D290V (GAT→GTT)	uspE ←	universal stress protein with a role cellular motility

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,397,754	0	T	A	100.0%	83.4 / NA	26	D290V (GAT→GTT)	uspE	universal stress protein with a role cellular motility
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (18/8);  total (18/8)

CAGTAAGTAAAAAATAGGCCCGATAACTCGGGCCTTGTCAGTTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTC  >  NC_000913/1397617‑1397887                                                                                                                                          |                                                                                                                                      cAGTAAGTAAAAAATAGGCCCGATAACTCGGGCCTTGTCAGTTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGaaca                                                                                                                                     <  2:522211/140‑1 (MQ=255)                  gCCCGATAACTCGGGCCTTGTCAGTTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGAACAATCACCTGTTCCGCCgt                                                                                                                    >  1:372026/1‑140 (MQ=255)                                gCCTTGTCAGTTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGAACAATCACCTGTTCCGCCGTGTTGcc                                                                                                              <  1:19317/132‑1 (MQ=255)                                gCCTTGTCAGTTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGAACAATCACCTGTTCCGCCGTGTTGcc                                                                                                              >  2:19317/1‑132 (MQ=255)                                 ccTTGTCAGTTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGAACAATCACCTGTTCCGCCGTGTTGCCGAGGAAtgc                                                                                                     <  2:683069/140‑1 (MQ=255)                                  cTTGTCAGTTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGAACAATCACCTGTTCCGCCGTGTTGCCGAGGAAtgct                                                                                                    <  1:125745/140‑1 (MQ=255)                                   ttGTCAGTTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGAACAATCACCTGTTCCGCCGTGTTGCCGAGGAAtgctg                                                                                                   >  1:236966/1‑140 (MQ=255)                                    tGTCAGTTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGAACAATCACCTGTTCCGCCGTGTTGCCGAGGAAtgctgc                                                                                                  >  1:589687/1‑140 (MQ=255)                                     gTCAGTTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGAACAATCACCTGTTCCGCCGTGTTGCCGAGGAATgctgct                                                                                                 >  2:17840/1‑140 (MQ=255)                                      tCAGTTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGAACAATCACCTGTTCCGCCGTGTTGCCGAGGAATGctgctg                                                                                                <  2:119559/140‑1 (MQ=255)                                          ttATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGAACAATCACCTGTTCCGCCGTGTTGCCGAGGAAtgc                                                                                                     <  1:170601/131‑1 (MQ=255)                                          ttATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGAACAATCACCTGTTCCGCCGTGTTGCCGAGGAAtgc                                                                                                     >  2:170601/1‑131 (MQ=255)                                                aaGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGAACAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGt                                                                                      <  2:372026/140‑1 (MQ=255)                                                  gagTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGAACAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGc                                                                                    >  1:607474/1‑140 (MQ=255)                                                    gTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGAACAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGt                                                                                  >  2:577632/1‑140 (MQ=255)                                                     tCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGAACAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTc                                                                                 >  2:408493/1‑140 (MQ=255)                                                               tcttctTCGTCATCCAGTTCAACGGGTGTCTGATCCTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGAACAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGt                                                                                      >  2:239554/1‑125 (MQ=255)                                                               tcttctTCGTCATCCAGTTCAACGGGTGTCTGATCCTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGAACAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGt                                                                                      <  1:239554/125‑1 (MQ=255)                                                                 ttcttcGTCATCCAGTTCAACGGGTGTCTGATAC‑GGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGAACAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAg                                                                    >  2:313270/1‑140 (MQ=255)                                                                            ccAGTTCAAGGGGTGTCTGAT‑CCGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATAAACAATCCCCTGTCCCGCCGTGTTGCGGAGGAATGCTGCTGAAATCCCGGTGCGTCCTCCCGTGCCCAAAACCACAAccc                                                         <  2:310692/140‑1 (MQ=255)                                                                                ttCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGAACAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAacaaca                                                             <  2:176644/133‑1 (MQ=255)                                                                                ttCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGAACAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACcaca                                                             >  1:176644/1‑133 (MQ=255)                                                                                ttCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGAACAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACcaca                                                             >  1:374576/1‑133 (MQ=255)                                                                                ttCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGAACAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACcaca                                                             <  2:374576/133‑1 (MQ=255)                                                                                          gtCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGAACAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAACCCCCGCCTGTAAGTg                                            <  2:607474/140‑1 (MQ=255)                                                                                               ttgcTGGTCAGTTTTAATGCCCAGCGGGTCGCGGGAAAGATGAACAACCACTTGTCCCGCGGTTTTGCCGGGGAATGCTCCGAAAATCCCGGTGCTCCCTCCCGGGCCCAAAACCACAATCCCCGCCTGTAAGTGCTCCg                                       <  2:363337/137‑1 (MQ=255)                                                                                                     gTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGAACAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAAt                                 >  2:122331/1‑140 (MQ=255)                                                                                                             tAATGACCAGCAGGTCGCAGCGAAGATGAACAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATc                         >  2:420395/1‑140 (MQ=255)                                                                                                                    cagcagGTCGCAGCGAAGATGAACAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCtctt                  >  2:713579/1‑140 (MQ=255)                                                                                                                              cAGCGAAGATGAACAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGATCCGCCAAATCAGGAATCACCTCTTCTGGCAGAc         >  2:278993/1‑139 (MQ=255)                                                                                                                              cAGCGAAGATGAACAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGATCCGCCAAATCAGGAATCACCTCTTCTGGCAGAc         <  1:278993/139‑1 (MQ=255)                                                                                                                                    aGATGAACAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTc  >  1:657453/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      CAGTAAGTAAAAAATAGGCCCGATAACTCGGGCCTTGTCAGTTATTGAAGAGTCGTTAATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCCGTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTC  >  NC_000913/1397617‑1397887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑