Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,953,436	Δ1 bp	coding (735/744 nt)	cmoA →	carboxy‑S‑adenosyl‑L‑methionine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,953,436	0	C	.	100.0%	72.9 / NA	18	coding (735/744 nt)	cmoA	carboxy‑S‑adenosyl‑L‑methionine synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (9/9);  total (9/9)

TGCTGGAAAACGTGATGCTGACCGATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAG  >  NC_000913/1953303‑1953561                                                                                                                                      |                                                                                                                              tGCTGGAAAACGTGATGCTGACCGATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGA‑GCTGCAt                                                                                                                        <  2:424657/140‑1 (MQ=255)        aaaCGTGATGCTGACCGATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGA‑GCTGCATGATCGAc                                                                                                                 <  2:95369/140‑1 (MQ=255)                                                cGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGA‑GCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCttt                                                                         >  2:317997/1‑140 (MQ=255)                                                             ccGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGA‑GCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGa                                                            >  2:142148/1‑140 (MQ=255)                                                                           aGTGAGCTGTGGTTCCAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGA‑GCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCgcgc                                              >  1:637701/1‑140 (MQ=255)                                                                           aGTGAGCTGTGGTTCCAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGA‑GCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCgcgc                                              <  1:142148/140‑1 (MQ=255)                                                                                    tgGTTCCAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGA‑GCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTa                                     >  2:343659/1‑140 (MQ=255)                                                                                    tgGTTCCAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGA‑GCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTACTa                                     >  1:295411/1‑140 (MQ=255)                                                                                         ccAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGA‑GCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTgg                                >  2:59941/1‑140 (MQ=255)                                                                                         ccAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGA‑GCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTgg                                >  2:617472/1‑140 (MQ=255)                                                                                            gTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGA‑GCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAg                             >  1:211707/1‑140 (MQ=255)                                                                                                tttAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGA‑GCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTACTAACTGGCAGcgcg                         <  2:295411/140‑1 (MQ=255)                                                                                                        tGGTTCACTGGTGGCATTAAAAGCAGAGGA‑GCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGagcagcag                 <  2:637701/140‑1 (MQ=255)                                                                                                         ggTTCACTGGTGGCATTAAAAGCAGAGGA‑GCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGagcagcagc                >  2:5859/1‑140 (MQ=255)                                                                                                             cACTGGTGGCATTAAAAGCAGAGGA‑GCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACgg            <  1:343659/140‑1 (MQ=255)                                                                                                             cACTGGTGGCATTAAAAGCAGAGGA‑GCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACgg            <  1:5859/140‑1 (MQ=255)                                                                                                             cACTGGTGGCATTAAAAGCAGAGGA‑GCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACgg            <  2:536989/140‑1 (MQ=255)                                                                                                                       aTTAAAAGCAGAGGA‑GCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAg  <  2:164221/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                              TGCTGGAAAACGTGATGCTGACCGATTCCGTGGAAACCCATAAAGCACGCCTGCATAACGCCGGTTTTGAGCATAGTGAGCTGTGGTTCCAGTGCTTTAACTTTGGTTCACTGGTGGCATTAAAAGCAGAGGACGCTGCATGATCGACTTTGGTAACTTTTATTCTCTGATTGCCAAAAATCATCTTTCACACTGGCTCGAAACGCTGCCCGCGCAGATTGCTAACTGGCAGCGCGAGCAGCAGCACGGGTTGTTTAAG  >  NC_000913/1953303‑1953561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑