Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,618,360	T→C	P139P (CCA→CCG)	hda ←	inibitor of reinitiation of DNA replication

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,618,360	0	T	C	100.0%	180.4 / NA	47	P139P (CCA→CCG)	hda	inibitor of reinitiation of DNA replication
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/23);  total (24/23)

AAAACCACGCAAACGCGCGCGTAACTGTAGCGCCTGCAACTTATCTTCATCAGAAAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATG  >  NC_000913/2618228‑2618494                                                                                                                                     |                                                                                                                                       aaaaCCACGCAAACGCGCGCGTAACTGTAGCGCCTGCAACTTATCTTCATCAGAAAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGAt                                                                                                                                 <  2:264586/140‑1 (MQ=255)       aCGCAAACGCGCGCGTAACTGTAGCGCCTGCAACTTATCTTCATCAGAAAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCgg                                                                                                                           <  1:375473/140‑1 (MQ=255)               gcgcgcgTAACTGTAGCGCCTGCAACTTATCTTCATCAGAAAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATcaac                                                                                                                   >  2:1258/1‑140 (MQ=255)                               cgcCTGCAACTTATCTTCATCAGAAAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCTAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCg                                                                                                   >  2:44445/1‑140 (MQ=255)                                 ccTGCAACTTATCTTCATCAGATAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGAt                                                                                                 >  2:669341/1‑140 (MQ=255)                                 ccTGCAACTTATCTTCATCAGAAAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGAt                                                                                                 >  1:129205/1‑140 (MQ=255)                                   tGCAACTTATCTTCATCAGAAAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTc                                                                                               >  1:633784/1‑140 (MQ=255)                                   tGCAACTTATCTTCATCAGAAAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTc                                                                                               >  2:586184/1‑140 (MQ=255)                                       aCTTATCTTCATCAGAAAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGa                                                                                           >  2:557693/1‑140 (MQ=255)                                        cTTATCTTCATCAGAAAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGaa                                                                                          <  2:136710/140‑1 (MQ=255)                                        cTTATCTTCATCAGAAAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGaa                                                                                          <  2:129205/140‑1 (MQ=255)                                          tATCTTCATCAGAAAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCTAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAAtt                                                                                        <  1:44445/140‑1 (MQ=255)                                          tATCTTCATCAGAAAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAAtt                                                                                        <  1:557693/140‑1 (MQ=255)                                                     gAAAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTagag                                                                             >  1:260815/1‑140 (MQ=255)                                                      aaaGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAgaga                                                                            >  2:667638/1‑140 (MQ=255)                                                       aaGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGAt                                                                           <  2:633784/140‑1 (MQ=255)                                                           ggCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGaa                                                                       >  1:52789/1‑140 (MQ=255)                                                             cTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGaaaa                                                                     >  2:169790/1‑140 (MQ=255)                                                                   tttGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCa                                                               <  2:52789/140‑1 (MQ=255)                                                                   tttGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCa                                                               <  1:669341/140‑1 (MQ=255)                                                                      gTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCAtct                                                            <  1:641512/140‑1 (MQ=255)                                                                               cccccAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCa                                                               <  1:21208/128‑1 (MQ=255)                                                                               cccccAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCa                                                               >  2:21208/1‑128 (MQ=255)                                                                               cccccAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACt                                                   >  2:452587/1‑140 (MQ=255)                                                                               cccccAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACt                                                   >  1:318005/1‑140 (MQ=255)                                                                                    agtcgagtcgCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCg                                              >  1:294325/1‑140 (MQ=255)                                                                                      tcgagtcgCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGcc                                            >  2:41539/1‑140 (MQ=255)                                                                                       cgagtcgCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCt                                           <  1:1258/140‑1 (MQ=255)                                                                                         agtcgCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGc                                         >  2:350525/1‑140 (MQ=255)                                                                                           tcgCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCaa                                       <  1:41539/140‑1 (MQ=255)                                                                                            cgcgacgcgaGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAAt                                      <  1:586184/140‑1 (MQ=255)                                                                                               gacgcgaGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATaca                                   <  1:350525/140‑1 (MQ=255)                                                                                                 cgcgaGATCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACt                                 <  1:667638/140‑1 (MQ=255)                                                                                                       aTCCGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAAtgt                           >  1:76064/1‑140 (MQ=255)                                                                                                          cGGTAATCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAAtgttgt                        <  2:76064/140‑1 (MQ=255)                                                                                                                tCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAAtgttgt                        >  2:127644/1‑134 (MQ=255)                                                                                                                tCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAAtgttgt                        <  1:127644/134‑1 (MQ=255)                                                                                                                tCCCAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATAc                  >  1:187846/1‑140 (MQ=255)                                                                                                                   cAGATTCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGa               <  2:649371/140‑1 (MQ=255)                                                                                                                       ttCAACTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAg           >  2:182277/1‑140 (MQ=255)                                                                                                                           aCTGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGAc       >  2:535296/1‑140 (MQ=255)                                                                                                                             tGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACaa     <  2:187846/140‑1 (MQ=255)                                                                                                                             tGCCGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACaa     <  1:169790/140‑1 (MQ=255)                                                                                                                               ccGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAg           >  2:459788/1‑132 (MQ=255)                                                                                                                               ccGCGGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAg           <  1:459788/132‑1 (MQ=255)                                                                                                                                cgcgGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATg  <  1:614576/140‑1 (MQ=255)                                                                                                                                cgcgGCGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATg  >  1:426292/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                       AAAACCACGCAAACGCGCGCGTAACTGTAGCGCCTGCAACTTATCTTCATCAGAAAGTGGCTGCAATTTGTAGATCTGCCCCCAGTCGAGTCGCGACGCGAGATCCGGTAATCCCAGATTCAACTGCCGCGGTGGACGATCGCCGGTGATCAACAACCGTGTTTTGCCCGATTCCAGAATTCGATTGTAGAGATCGAAAATCGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATG  >  NC_000913/2618228‑2618494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑