Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,798,780	A→G	intergenic (‑285/‑384)	stpA ← / → alaE	DNA‑binding transcriptional repressor StpA with RNA chaperone activity/L‑alanine exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,798,780	0	A	G	100.0%	163.0 / NA	42	intergenic (‑285/‑384)	stpA/alaE	DNA‑binding transcriptional repressor StpA with RNA chaperone activity/L‑alanine exporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/21);  total (21/21)

ATTTCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGACCCAGCTACTACATACACCGAATTCTCTACGCTTATAGCGTTACCTCACCCCCAAACTTTCATTAGTGAT  >  NC_000913/2798643‑2798914                                                                                                                                          |                                                                                                                                       atTTCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGca                                                                                                                                      >  2:70338/1‑140 (MQ=255)        gATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCa                                                                                                                               <  2:642887/140‑1 (MQ=255)        gATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCa                                                                                                                               <  2:524072/140‑1 (MQ=255)           tatataTTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTg                                                                                                                            <  1:98602/140‑1 (MQ=255)                  tAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCttt                                                                                                                     >  1:637317/1‑140 (MQ=255)                   aGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTc                                                                                                                    >  1:630834/1‑140 (MQ=255)                         tttGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGt                                                                                                              >  1:132247/1‑140 (MQ=255)                         tttGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGt                                                                                                              >  2:165685/1‑140 (MQ=255)                          ttGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGtt                                                                                                             <  2:480863/140‑1 (MQ=255)                           tgatgaGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTa                                                                                                            <  1:165685/140‑1 (MQ=255)                                  tAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACa                                                                                                     <  2:223241/140‑1 (MQ=255)                                  tAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACa                                                                                                     <  2:637317/140‑1 (MQ=255)                                     tttAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTg                                                                                                  >  2:330430/1‑140 (MQ=255)                                                  tGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATaaaaa                                                                                     <  1:86647/140‑1 (MQ=255)                                                         agCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGa                                                                              >  1:458920/1‑140 (MQ=255)                                                         agCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGa                                                                              >  2:284894/1‑140 (MQ=255)                                                         agCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGa                                                                              >  2:264952/1‑140 (MQ=255)                                                            ggcgCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGaaaa                                                                           >  1:240799/1‑140 (MQ=255)                                                               gcTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGa                                                                        <  1:442314/140‑1 (MQ=255)                                                                 tctctGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGAcc                                                                      <  2:407668/140‑1 (MQ=255)                                                                  ctctGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGAccc                                                                     <  2:132247/140‑1 (MQ=255)                                                                    ctGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGACCCAg                                                                   <  2:628728/140‑1 (MQ=255)                                                                               ttCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGAccc                                                                     >  2:300203/1‑127 (MQ=255)                                                                               ttCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGAccc                                                                     <  1:300203/127‑1 (MQ=255)                                                                               ttCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGACCCAGCTACtacatac                                                        >  1:124615/1‑140 (MQ=255)                                                                                    cAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGACCCAGCTACTACATACACCGa                                                   <  2:510926/140‑1 (MQ=255)                                                                                    cAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGACCCAGCTACTACATACACCGa                                                   <  2:458920/140‑1 (MQ=255)                                                                                                 aTCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGACCCAGCTACTACATACACCGAATTCTCTACGCtt                                      >  1:328649/1‑140 (MQ=255)                                                                                                  tCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGACCCAGCTACTACATACACCGAATTCTCTACGCTta                                     <  2:97342/140‑1 (MQ=255)                                                                                                  tCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGACCCAGCTACTACATACACCGAATTCTCTACGCTta                                     <  2:117924/140‑1 (MQ=255)                                                                                                        tGAATAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGACCCAGCTACTACATACACCGAATTCTCTACGCTTATAGCGt                               >  1:215158/1‑140 (MQ=255)                                                                                                          aaTAAAAATGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGACCCAGCTACTACATACACCGAATTCTCTACGCTTATAGCGTTa                             >  2:11104/1‑140 (MQ=255)                                                                                                             aaaaaTGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGACCCAGCTACTACATACACCGAATTCTCTACGCTTATAGCGTTACCt                          <  1:639476/140‑1 (MQ=255)                                                                                                                  tGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGACCCAGCTACTACATACACCGAATTCTCTACGCTtat                                    >  2:576933/1‑125 (MQ=255)                                                                                                                  tGCAATATAACAGAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGACCCAGCTACTACATACACCGAATTCTCTACGCTtat                                    <  1:576933/125‑1 (MQ=255)                                                                                                                              gAATAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGACCCAGCTACTACATACACCGAATTCTCTACGCTTATAGCGTTACCTCACCCCCAAACTTTCAt         >  2:617185/1‑140 (MQ=255)                                                                                                                                 tAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGACCCAGCTACTACATACACCGAATTCTCTACGCTTATAGCGTTACCTCACCCCCAAACTTTCATTAg      <  2:315559/140‑1 (MQ=255)                                                                                                                                 tAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGACCCAGCTACTACATACACCGAATTCTCTACGCTTATAGCGTTACCTCACCCCCAAACTTTCATTAg      >  2:461877/1‑140 (MQ=255)                                                                                                                                 tAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGACCCAGCTACTACATACACCGAATTCTCTACGCTTATAGCGTTACCTCACCCCCAAACTTTCATTAg      <  2:240799/140‑1 (MQ=255)                                                                                                                                 tAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGACCCAGCTACTACATACACCGAATTCTCTACGCTTATAGCGTTACCTCACCCCCAAACTTTCATTAg      >  2:590/1‑140 (MQ=255)                                                                                                                                 tAAAAGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGACCCAGCTACTACATACACCGAATTCTCTACGCTTATAGCGTTACCTCACCCCCAAACTTTCATTAg      >  1:580365/1‑140 (MQ=255)                                                                                                                                     aGAAAGCATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGACCCAGCTACTACATACACCGAATTCTCTACGCTTATAGCGTTACCTCACCCCCAAACTTTCATTAGTGat  >  1:369642/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       ATTTCAAGATTATATATTAGCCATTTTGATGAGTAATTTAGATTATTGGTGAACAGAGCGGCGCTCTCTGCTCGCTAATTCCGCAAGCCTGACAAAATCAAAGTGAATAAAAATGCAATATAACAGAATAAAAGAAAACATCCATCACTGGTGCTTTCTGCTGTTAATGTACATTGTCTGGCATAAAAAATGCTGAAAATGACCCAGCTACTACATACACCGAATTCTCTACGCTTATAGCGTTACCTCACCCCCAAACTTTCATTAGTGAT  >  NC_000913/2798643‑2798914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑