Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913372,6400TG23.5% 141.4 / 12.4 68L176V (TTA→GTA) mhpD2‑hydroxypentadienoate hydratase
Reads supporting (aligned to +/- strand):  ref base T (20/32);  new base G (16/0);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 5.38e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CATTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCA‑CGCAAAATGGCCAGTCTGGGTGAACCGCT  >  NC_000913/372501‑372779
                                                                                                                                           |                                                                                                                                            
cATTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTGCGGAGCAACTGCCGGGGGGGt                                                                                                                                              >  2:259951/1‑140 (MQ=255)
cATTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGt                                                                                                                                              <  2:1103130/140‑1 (MQ=255)
cATTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGt                                                                                                                                              <  1:1329365/140‑1 (MQ=255)
cATTGAATGGGTACTTCCGGCACTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGt                                                                                                                                              <  2:389113/140‑1 (MQ=255)
  ttGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTa                                                                                                                                            >  2:333748/1‑140 (MQ=255)
  ttGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTa                                                                                                                                            >  1:369088/1‑140 (MQ=255)
  ttGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGCCCGGCGCAACGTCCGGCGGGGTTa                                                                                                                                            >  2:1664316/1‑140 (MQ=255)
   tGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAg                                                                                                                                           <  1:1659228/140‑1 (MQ=255)
   tGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAg                                                                                                                                           <  2:1592725/140‑1 (MQ=255)
   tGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGACACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAg                                                                                                                                           <  1:675636/140‑1 (MQ=255)
          gTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCATATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGaa                                                                                                                                    >  1:1751871/1‑140 (MQ=255)
          gTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGaa                                                                                                                                    <  2:646870/140‑1 (MQ=255)
          gTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGAACTGaa                                                                                                                                    >  2:994705/1‑140 (MQ=255)
          gTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGAACTGaa                                                                                                                                    >  2:1283027/1‑140 (MQ=255)
          gTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGGCCGGCGGGGGTAGAGCTGaa                                                                                                                                    >  2:389071/1‑140 (MQ=255)
               tCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGaaaaaat                                                                                                                               >  1:1809744/1‑138 (MQ=255)
                ccagcgcCGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGGCCGGCGGGGGTAGACCTGAAAAAcag                                                                                                                              >  2:1405856/4‑138 (MQ=255)
                   gcgcTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACTTGAAAAACTgcgc                                                                                                                           >  1:1166095/1‑140 (MQ=255)
                   gcgcTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTgcgc                                                                                                                           >  1:1549008/1‑140 (MQ=255)
                     gcTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCa                                                                                                                         <  1:259951/140‑1 (MQ=255)
                        ggAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGa                                                                                                                      >  1:1373938/1‑140 (MQ=255)
                             tggtggGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATg                                                                                                                 <  1:893688/140‑1 (MQ=255)
                              ggtggGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGa                                                                                                                <  2:49841/140‑1 (MQ=255)
                               gtggGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGAc                                                                                                               <  1:36534/140‑1 (MQ=255)
                                tggGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGAcg                                                                                                              <  1:1140478/140‑1 (MQ=255)
                                  gggAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGGTAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGAcgcg                                                                                                            <  1:111239/140‑1 (MQ=255)
                                  gggAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGAcgcg                                                                                                            <  2:182357/140‑1 (MQ=255)
                                  gggAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGAcgcg                                                                                                            <  2:1376803/140‑1 (MQ=255)
                                   ggAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGAcg                                                                                                              <  2:564782/137‑1 (MQ=255)
                                   ggAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGAcg                                                                                                              >  1:564782/1‑137 (MQ=255)
                                   ggAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGGCCGGCGGGGGTAGACCTGAAAAAATGCGCCATGGAGATGAcgcgg                                                                                                           >  1:488193/1‑139 (MQ=255)
                                    gAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCCGCCCAACGTCCGGCGGGGGTAGACCTGAAAAAAAGCGCCATGAAGATTAcgcgga                                                                                                          >  2:1049400/1‑138 (MQ=255)
                                      gCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGtaat                                                                                                        >  1:1666809/1‑140 (MQ=255)
                                       ccGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGtaata                                                                                                       >  1:233491/1‑140 (MQ=255)
                                          cATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACg                                                                                                    >  1:1270278/1‑140 (MQ=255)
                                          cATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGtaataaag                                                                                                    >  1:974229/1‑138 (MQ=255)
                                            ttCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGaa                                                                                                  <  1:1044825/140‑1 (MQ=255)
                                            ttCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGaa                                                                                                  <  1:1525396/140‑1 (MQ=255)
                                             tCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAag                                                                                                 <  1:922214/140‑1 (MQ=255)
                                              cgcgACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAaga                                                                                                >  1:1655341/1‑140 (MQ=255)
                                                         gATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCg                                                                                     >  2:640257/1‑140 (MQ=255)
                                                         gATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCg                                                                                     >  1:1653276/1‑140 (MQ=255)
                                                         gATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTTGACCCGAAAAACCGCGCCATGGAAATGACGCGCAATAACGAAGAGGGTTCTAGCg                                                                                     >  2:862815/1‑140 (MQ=255)
                                                                     cGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCg                                                                         <  2:666432/140‑1 (MQ=255)
                                                                      gATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGa                                                                        <  1:1083471/140‑1 (MQ=255)
                                                                      gATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGa                                                                        <  1:333748/140‑1 (MQ=255)
                                                                         aCCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATg                                                                     >  1:501540/1‑140 (MQ=255)
                                                                          ccGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGc                                                                    >  1:1276691/1‑140 (MQ=255)
                                                                             tGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTg                                                                 >  2:651713/1‑140 (MQ=255)
                                                                              ggCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCGGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGGCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTgg                                                                <  1:833501/140‑1 (MQ=255)
                                                                                  gATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACa                                                            <  2:1578139/140‑1 (MQ=255)
                                                                                          ctcctGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTa                                                    >  2:1353915/1‑140 (MQ=255)
                                                                                            cctGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAAt                                                  >  1:1248783/1‑140 (MQ=255)
                                                                                              tgtgGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGc                                                <  2:1270278/140‑1 (MQ=255)
                                                                                              tgtgGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGc                                                <  1:640257/140‑1 (MQ=255)
                                                                                               gtgGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCg                                               <  2:256859/140‑1 (MQ=255)
                                                                                                  gggTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCCTGAAGATGAAGCGTAATAAAGAAGAGGGTTCTAGCGGGCGCGGCAGCGGATGCCTGGGGCATCCGCTTAATGCGGcc                                            >  2:1435881/1‑140 (MQ=255)
                                                                                                      gtATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTct                                        <  2:1031104/140‑1 (MQ=255)
                                                                                                       tATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTTGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTctg                                       >  2:1440240/1‑140 (MQ=255)
                                                                                                       tATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCCTGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTctg                                       >  1:1446231/1‑140 (MQ=255)
                                                                                                       tATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTctg                                       >  1:236318/1‑140 (MQ=255)
                                                                                                        aTGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTctgg                                      <  2:369088/140‑1 (MQ=255)
                                                                                                        aTGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTctgg                                      <  2:1809744/140‑1 (MQ=255)
                                                                                                          gTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTctggct                                    >  2:792848/1‑140 (MQ=255)
                                                                                                              tCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTTTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCa                                >  2:1088694/1‑140 (MQ=255)
                                                                                                              tCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGAACTGAAAAACTGCGCCCTGAAGATGACGCGGAAAAACGGAGAGGTTTCTAGCGGGGGCGGCAGCCAATGCCCGGGACATCCGCTTAATGTGGCCGCCTTGCTGGCa                                >  2:533006/1‑140 (MQ=255)
                                                                                                               cggcggTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCA‑c                              >  1:1200980/1‑140 (MQ=255)
                                                                                                                 gcggTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCA‑CGc                            >  1:1589726/1‑140 (MQ=255)
                                                                                                                 gcggTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCA‑CGc                            >  1:1525559/1‑140 (MQ=255)
                                                                                                                 gcggTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCA‑CGc                            >  1:1716632/1‑140 (MQ=255)
                                                                                                                  cggTCCGGCGCAACCTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATGCATGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCA‑CGCa                           >  1:848110/1‑140 (MQ=255)
                                                                                                                     tCCGGCGCAACGTCCGGCGGGGTTTGAACCGAAAAACTGCGCCATGAAAGTGACGCGTAAAAAAGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTTGGGCATCCGCCTAATGCGGGCGGCTCGCTGGTACCGCaaa                         >  2:840888/1‑140 (MQ=255)
                                                                                                                     tCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCA‑CGCaaaa                        >  1:691648/1‑140 (MQ=255)
                                                                                                                     tCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAAAGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGGCATCCGCTTAAAGCGGCCGTCTGGCTGGCA‑CGCaaaa                        >  1:1510281/1‑140 (MQ=255)
                                                                                                                       cGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCA‑CGCAAAATg                      <  1:1405856/140‑1 (MQ=255)
                                                                                                                         gcgcAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCA‑CGCAAAATGGc                    <  1:1103770/140‑1 (MQ=255)
                                                                                                                         gcgcAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCA‑CGCAAAATGGc                    <  1:792848/140‑1 (MQ=255)
                                                                                                                            cAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCA‑CGCAAAATGGCCAg                 <  1:651713/140‑1 (MQ=255)
                                                                                                                               cGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCA‑CGCAAAATGGCCAGTCt              >  2:453905/1‑140 (MQ=255)
                                                                                                                               cGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCA‑CGCAAAATGGCCAGTCt              >  2:1180674/1‑140 (MQ=255)
                                                                                                                                   cggcggGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCA‑CGCAAAATGGCCAGTCTGGGt          >  2:288032/1‑140 (MQ=255)
                                                                                                                                      cggGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCA‑CGCAAAATGGCCAGTCTGGGTGaa       <  1:862815/140‑1 (MQ=255)
                                                                                                                                          gTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCA‑CGCAAAGTGGCCAGTCTGGGTGAACCGc   >  2:1577598/1‑140 (MQ=255)
                                                                                                                                           ttAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCA‑CGCAAAATGGCCAGTCTGGGTGAACCGCt  <  2:1446231/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
CATTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCA‑CGCAAAATGGCCAGTCTGGGTGAACCGCT  >  NC_000913/372501‑372779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑