Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,692,869 T→G intergenic (‑18/+21) uidC ← / ← uidB outer membrane porin family protein/glucuronide:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,692,8690TG98.1% 186.6 / ‑4.1 52intergenic (‑18/+21)uidC/uidBouter membrane porin family protein/glucuronide:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (25/26);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01

GTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAAT  >  NC_000913/1692735‑1693004
                                                                                                                                      |                                                                                                                                       
gTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGTCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGat                                                                                                                                    <  2:433190/140‑1 (MQ=255)
        gTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTa                                                                                                                            <  1:389109/140‑1 (MQ=255)
          cTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTAtt                                                                                                                          <  2:1079711/140‑1 (MQ=255)
          cTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTAtt                                                                                                                          <  1:819038/140‑1 (MQ=255)
                    gTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGTATattaat                                                                                                                <  2:32209/140‑1 (MQ=255)
                     tCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATattaatt                                                                                                               >  1:334080/1‑140 (MQ=255)
                     tCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATattaatt                                                                                                               <  1:946764/140‑1 (MQ=255)
                       ccGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAg                                                                                                             >  2:920719/1‑140 (MQ=255)
                       ccGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAATTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAg                                                                                                             >  1:731246/1‑140 (MQ=255)
                         gCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTg                                                                                                           <  2:767083/140‑1 (MQ=255)
                               tCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCg                                                                                                     <  1:1018219/140‑1 (MQ=255)
                               tCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCg                                                                                                     >  2:697534/1‑140 (MQ=255)
                               tCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCg                                                                                                     >  2:954021/1‑140 (MQ=255)
                                   cAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGa                                                                                                 <  1:73460/140‑1 (MQ=255)
                                     gCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGAtt                                                                                               <  1:816735/140‑1 (MQ=255)
                                        aGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAAt                                                                                            <  2:926514/140‑1 (MQ=255)
                                                cgcATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATtgctgctg                                                                                    <  1:920719/140‑1 (MQ=255)
                                                 gcATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATtgctgctgc                                                                                   <  2:493672/140‑1 (MQ=255)
                                                            ggTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACg                                                                        >  2:566407/1‑140 (MQ=255)
                                                            ggTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACg                                                                        >  2:983575/1‑140 (MQ=255)
                                                             gTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGa                                                                       >  1:706944/1‑140 (MQ=255)
                                                             gTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGa                                                                       >  1:228949/1‑140 (MQ=255)
                                                                aGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTa                                                                    <  1:697534/140‑1 (MQ=255)
                                                                       agGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAAttt                                                             >  1:503413/1‑140 (MQ=255)
                                                                       agGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAAttt                                                             >  1:876210/1‑140 (MQ=255)
                                                                       agGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAAttt                                                             >  2:864936/1‑140 (MQ=255)
                                                                       agGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAAttt                                                             >  2:536160/1‑140 (MQ=255)
                                                                            gCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAAcc                                                        >  2:837044/1‑140 (MQ=255)
                                                                            gCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAAcc                                                        >  1:672700/1‑140 (MQ=255)
                                                                                tCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGa                                                    <  1:409963/140‑1 (MQ=255)
                                                                                          aaCGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGaa                                          <  2:876210/140‑1 (MQ=255)
                                                                                                   ggCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATc                                 >  1:167382/1‑140 (MQ=255)
                                                                                                   ggCCACTATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATc                                 >  1:683569/1‑140 (MQ=255)
                                                                                                         tATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAg                           <  1:373629/140‑1 (MQ=255)
                                                                                                         tATTTTTCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAg                           <  1:467491/140‑1 (MQ=255)
                                                                                                         tATTTTTCTCATAATTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAg                           <  2:731246/140‑1 (MQ=255)
                                                                                                             tttCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGa                       <  2:1061750/140‑1 (MQ=255)
                                                                                                             tttCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGa                       <  2:934907/140‑1 (MQ=255)
                                                                                                             tttCTCATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGa                       <  1:746597/140‑1 (MQ=255)
                                                                                                                ctcATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATaa                    <  2:672700/140‑1 (MQ=255)
                                                                                                                ctcATAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATaa                    <  2:334080/140‑1 (MQ=255)
                                                                                                                   aTAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCACCCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAAc                 <  2:278150/140‑1 (MQ=255)
                                                                                                                   aTAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAAc                 <  2:683569/140‑1 (MQ=255)
                                                                                                                   aTAGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAAc                 >  1:713727/1‑140 (MQ=255)
                                                                                                                     aGTTGCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCa               <  1:864936/140‑1 (MQ=255)
                                                                                                                         gCACCTTTGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGata           >  2:768682/1‑140 (MQ=255)
                                                                                                                               ttGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACg     >  2:760534/1‑140 (MQ=255)
                                                                                                                               ttGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACg     >  2:842359/1‑140 (MQ=255)
                                                                                                                                tGATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTTCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGa    >  1:833517/1‑140 (MQ=255)
                                                                                                                                 gATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGaa   >  2:173685/1‑140 (MQ=255)
                                                                                                                                 gATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGaa   >  2:453828/1‑140 (MQ=255)
                                                                                                                                 gATGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGaa   >  2:581617/1‑140 (MQ=255)
                                                                                                                                  aTGTGCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAAt  >  2:345628/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                       
GTTTTTCAGTCTGATGCGTAGTCCCGCTTCATCGTCAGCCAGTTGCGCCGCATAAGCAGAGGTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAAT  >  NC_000913/1692735‑1693004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑