| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 650,809 | 0 | T | G | 26.3% | 71.7 / 10.7 | 38 | T350P (ACC→CCC) | citC | citrate lyase synthetase |
| Reads supporting (aligned to +/- strand): ref base T (6/22); new base G (10/0); total (16/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.69e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTC‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAG > NC_000913/650672‑650940 | cAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCt > 1:432032/1‑140 (MQ=255) tGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTg < 1:1069048/140‑1 (MQ=255) tttGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGCATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCcg < 2:400644/140‑1 (MQ=255) gCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGt < 1:559231/140‑1 (MQ=255) cAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGCGCCTGCAACGGCGGGCTGGTTTATTTTTCTTTTTCTACTGTTTCTCATGCGGGGGGCTTTTGTCGCACTGCCGCGTc > 2:1041566/1‑140 (MQ=255) tatCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCggg < 1:460692/140‑1 (MQ=255) tGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCgggggggg > 2:866450/1‑135 (MQ=255) gCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGTGCt > 1:890117/1‑140 (MQ=255) cGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTc > 1:24815/1‑140 (MQ=255) cGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTCTCCTGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGGTC‑AAGCa > 2:366116/1‑140 (MQ=255) gTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGTGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTC‑AAGCAGGt < 1:1041566/140‑1 (MQ=255) gggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTC‑AAGCAGGTTCt < 1:447827/140‑1 (MQ=255) ggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTTATTTTTTACCTGTTTTTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTC‑AAGCAGGGTCTg > 1:876629/1‑140 (MQ=255) gCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTC‑AAGCAGGTTCTGc < 2:1067622/140‑1 (MQ=255) tGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGTGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTC‑AAGCAGGTTCTGCAaat > 1:1090478/1‑140 (MQ=255) catcaCATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTC‑AAGCAGGTTCTGCAAATAATGCAg < 1:847245/140‑1 (MQ=255) cacaTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTC‑CAGGAGGGTCTGCCAAAAATTCAGCgg > 2:892785/1‑139 (MQ=255) cacaTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTTACCTGTTTTTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTC‑AAGCAAGGTGTGCAAAAAATGCAGCgg > 2:870159/1‑139 (MQ=255) caTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTTTTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTC‑AAGCAGGTTCTGCAAATAATGCAGCGTGa > 1:1023944/1‑140 (MQ=255) tCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGGTC‑AGGCAGGTTCTGCAAAAAATGCAGCGGGAcc > 2:802557/1‑139 (MQ=255) ccccAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTTATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGGTC‑AAGCAGGGTTTTCAAAAAAAGCAGCGGGGCTg > 2:567483/1‑140 (MQ=255) ccAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTC‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCa < 1:892785/140‑1 (MQ=255) cAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTTTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGGTC‑AAGCCGGGTCTGCCAAAAATTCAGCGGGACTGccg > 2:665995/1‑138 (MQ=255) gACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTC‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAggg > 1:584726/1‑140 (MQ=255) ggTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTC‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAgcgg < 1:567483/140‑1 (MQ=255) ggTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTC‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAgcgg < 2:24815/140‑1 (MQ=255) tGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTC‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAgcggcg < 1:1088583/140‑1 (MQ=255) tGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTC‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAgcggcg < 1:866450/140‑1 (MQ=255) ccTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTC‑AGGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGcgcg < 1:802557/140‑1 (MQ=255) ccTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTC‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGcgcg < 2:606590/140‑1 (MQ=255) ccTGCAACGGCGGGCTGGGTTATTTTTATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGTGCTC‑AAGCAGGGTTTTCCAATAATGCAGCGCGGCTGCCGGGGCCAGCGGggcg > 2:1005014/1‑140 (MQ=255) cTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTC‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGa < 2:584726/140‑1 (MQ=255) cTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTC‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGa < 1:870159/140‑1 (MQ=255) cggGCTGGGTTATTTTCATTTTTTACCTGTTTCTCATTGGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGGTCAAACCAGGTT‑TGCAAAAAATGGAGCGTGACTCGAGGGGCCACCGGCGCGGGAGGCGTgg > 2:756132/1‑139 (MQ=255) cggGCTGGGTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGTGGTC‑CAACAGGTTCTGCAAATAATGCAGCGGGACTGCAGGGGCCAGCGGCGCGATAGCCgggg > 2:37552/1‑137 (MQ=255) tttCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTC‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGc < 2:1090478/140‑1 (MQ=255) tttCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTC‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCA‑GGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGcc > 2:76436/1‑140 (MQ=255) ttCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTC‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGcc < 2:884348/140‑1 (MQ=255) aTTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTC‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCcagc > 2:237012/1‑140 (MQ=255) aTTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTC‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCcagc < 2:1040610/140‑1 (MQ=255) ccTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTC‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACg < 2:432032/140‑1 (MQ=255) tGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTC‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAg < 1:1005014/140‑1 (MQ=255) | CAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTC‑AAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAG > NC_000913/650672‑650940 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |