Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,101,5070TG35.3% 80.7 / 10.4 51intergenic (+77/‑183)sodA/kdgTsuperoxide dismutase (Mn)/2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (8/25);  new base G (18/0);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 7.32e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGG  >  NC_000913/4101374‑4101646
                                                                                                                                     |                                                                                                                                           
ttCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGttttttt                                                                                                                                       >  1:766158/1‑140 (MQ=255)
ttCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGgtttttt                                                                                                                                       >  1:606491/1‑140 (MQ=255)
 tCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTtg                                                                                                                                      <  2:215047/140‑1 (MQ=255)
  cTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAGCGGCGGTGGttttttttt                                                                                                                                     >  1:1077699/1‑138 (MQ=255)
  cTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTtgt                                                                                                                                     >  1:43255/1‑140 (MQ=255)
   tGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTtttg                                                                                                                                    >  2:217168/1‑140 (MQ=255)
          tggtgAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAAtt                                                                                                                             >  2:882413/1‑140 (MQ=255)
               aaCTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGGTCAATTTCaaa                                                                                                                        >  2:184513/1‑140 (MQ=255)
                   gggACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATaa                                                                                                                    <  1:305817/140‑1 (MQ=255)
                    ggACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTGTAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGGGGGTTTTTTTTGTTCAATTTTaaaaaaaa                                                                                                                   >  2:68713/1‑140 (MQ=255)
                         aaGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATCAATTTTAaaaaaaaaaaaa                                                                                                              >  2:636191/1‑140 (MQ=255)
                                  cACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGat                                                                                                     <  1:630484/140‑1 (MQ=255)
                                    cGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGataa                                                                                                   >  1:123445/1‑140 (MQ=255)
                                              cgAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACa                                                                                         <  1:84940/140‑1 (MQ=255)
                                                aaaaaaTAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGCGGGTTTTTTTTTATTAATTTTAAAAAAAAAACAAAGATCCGaaaaaaaaaaaaaaac                                                                                       >  1:558202/1‑136 (MQ=255)
                                                  aaaaTAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAGCGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGt                                                                                     <  2:1077699/140‑1 (MQ=255)
                                                        atCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAAt                                                                               <  2:989349/140‑1 (MQ=255)
                                                             ttGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGAtg                                                                          <  2:984246/140‑1 (MQ=255)
                                                             ttGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGAtg                                                                          <  1:425664/140‑1 (MQ=255)
                                                             ttGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGAtg                                                                          <  1:882413/140‑1 (MQ=255)
                                                             ttGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGAtg                                                                          <  2:766158/140‑1 (MQ=255)
                                                                  gccTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGtt                                                                     <  2:1144435/140‑1 (MQ=255)
                                                                      gctgcAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGa                                                                 <  2:43255/140‑1 (MQ=255)
                                                                       ctgcAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGGTTAATTTTAAAATAAAAAAAATGAACCGATAAAAAAAAAAAAACGTTTTAATTGATGTGGTTTTTAt                                                                >  2:881178/1‑140 (MQ=255)
                                                                       ctgcAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTGTGATCAATTTTAAAATAAAAAAAATGATCCGATAAAAATAAAAAAGCGGTTTAATTGATGTGGTTTTGAt                                                                >  1:179237/1‑140 (MQ=255)
                                                                          cAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCAc                                                             <  1:636191/140‑1 (MQ=255)
                                                                              gAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATCAATTTTAAAATAAAAAAAAAGATCCGATAAAAATAAAAAAACGGTTTAATTTATGTGGTTTTTATTACtttt                                                         >  2:455672/1‑140 (MQ=255)
                                                                                     ataGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGAtt                                                  <  2:123445/140‑1 (MQ=255)
                                                                                     ataGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGAtt                                                  <  1:455672/140‑1 (MQ=255)
                                                                                           cGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTTTGGTCAATTTTAAAATAAAAACAAAGATCCGATAAAAAAAAAAAAGCGTTTCAATTGATGTGGTTTTGATCAATTTTATTTATTAaatga                                            >  2:878660/1‑140 (MQ=255)
                                                                                           cGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTTTGATCAATTTTAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGGTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAaatga                                            >  1:965516/1‑140 (MQ=255)
                                                                                           cGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAaatga                                            <  2:125013/140‑1 (MQ=255)
                                                                                           cGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATCAATTTTTAAATAAAAAAAAAGGTCCGATAAAAATAAAAAAGCGGTTTAATTTATTTGGTTTTTATTACTTTTTTTTATTAaatta                                            >  2:1107271/1‑138 (MQ=255)
                                                                                           cGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATCAATTTTAAAATAAAAAAAAAGATCCGATAAAAAAAAAAAAGCGTTTTAATTTATGTGGGTTTTATCACTTTTTTTTATTAaatga                                            >  2:1033219/1‑140 (MQ=255)
                                                                                           cGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAAAAAAAAAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAaatga                                            >  1:468177/1‑140 (MQ=255)
                                                                                           cGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTGTGATCAATTTCAAAATAAAAACAAAGATCCGATAAAAATAAAACAGCGGTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAaatga                                            >  1:821361/1‑140 (MQ=255)
                                                                                            gCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAaatgaa                                           >  1:778392/1‑140 (MQ=255)
                                                                                              atatCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATCAATTTTAAAAAAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAaaagaatg                                         >  1:992748/1‑140 (MQ=255)
                                                                                              atatCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATCAATTTCAAAATAAAAAAAATGATCCGATAAAAATAAAAAAACGTTTTAATTTATGTGGGTTTTATCACTTTTATTTATTAaatgaatg                                         >  2:457939/1‑140 (MQ=255)
                                                                                                     cTTAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATCAATTTTAAAATAAAAACAAAGATTCGATAAAAATAAAACAACGGTTTAATTTATGTGGGTTTTATTACTTTTATTTATTAAATGAATGTCTATCt                                  >  2:508095/1‑140 (MQ=255)
                                                                                                       tAAAAAATGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTTATCACTTTTATTGATTAAATGAATGTCTATCTTc                                >  1:81057/1‑140 (MQ=255)
                                                                                                        aaaaaaTGAACCATCGCCAACGGCGGTGGTTTTTTTTTGATCAATTTTAAAATTAAAAAAAACATCCGCTAAAAATAAAACAGCGGTTCAAATTTTGTGGTTTTGATGACTTTTCTTTAATAAATCAATGTTTATCTTct                               >  2:97178/1‑139 (MQ=255)
                                                                                                          aaaaTGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGtt                             <  2:778392/140‑1 (MQ=255)
                                                                                                          aaaaTGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGtt                             <  1:878660/140‑1 (MQ=255)
                                                                                                          aaaaTGAACCATCGCCAACGGCGGTGGGTTTTTTTTGATCAATTTTAAAAAAAAAACAAAGATCCGATAAAAAAAAAACAACGGTTTAATTTATGTGGTTTTTATCACCTTTTTTGGTTAAATAAAGGTCTTTCTTCGtt                             >  2:822969/1‑140 (MQ=255)
                                                                                                                      cGCCAACGGCGGTGGTTTTTTTGTGATCAATTTTAAAATAAAAAAAAAGATCCGATAAAAATAAAACAGCGTTTTAATTTATGTGGTTTTTATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACt                 >  2:46157/1‑140 (MQ=255)
                                                                                                                          aaCGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATg             <  2:566729/140‑1 (MQ=255)
                                                                                                                          aaCGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATg             <  2:609306/140‑1 (MQ=255)
                                                                                                                          aaCGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATg             <  2:1015688/140‑1 (MQ=255)
                                                                                                                            cggcggTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCt           <  1:953979/140‑1 (MQ=255)
                                                                                                                             ggcggTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTc          <  1:457939/140‑1 (MQ=255)
                                                                                                                             ggcggTGGTCTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACAGATGCTc          <  2:106379/140‑1 (MQ=255)
                                                                                                                               cggTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCa        <  2:466218/140‑1 (MQ=255)
                                                                                                                               cggTGGTTTTTTTGTGATCAATTTCAAAATAAAAAAAATGATCCGCTAAAAAAAAAAAAGCGTTTCAATTGATTTGGTTTTTATCACTTTTATTGATTAAATGAAAGTCTATCTTCGTTTTCCTCAAAACTGATGCTccc        >  2:1046589/1‑139 (MQ=255)
                                                                                                                                     tttttttGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAgg  <  1:881178/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                           
TTCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGTGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGG  >  NC_000913/4101374‑4101646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑