| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 646,747 | 0 | T | G | 20.0% | 95.9 / 11.3 | 40 | T255P (ACC→CCC) | citG | triphosphoribosyl‑dephospho‑CoA synthase |
| Reads supporting (aligned to +/- strand): ref base T (0/32); new base G (8/0); total (8/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.30e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTT > NC_000913/646608‑646884 | aaaTTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGt < 2:941820/140‑1 (MQ=255) gCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCt < 2:937696/140‑1 (MQ=255) cTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGAATGcccccct > 1:792027/1‑138 (MQ=255) cTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGAATGCCCCCtt > 1:891847/1‑140 (MQ=255) aataatTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTGGGATGCCCCCcttttt > 2:608756/1‑139 (MQ=255) atTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGGATGCCCCCCTTTTTCaat > 1:695149/1‑140 (MQ=255) ttAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTCGACGTCCCCCttttttaata > 2:450506/1‑135 (MQ=255) aaaTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGc < 1:437124/123‑1 (MQ=255) aaaTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGAATGc > 2:437124/1‑123 (MQ=255) tCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTTGAATGCCCCCCTTTTTtaataaattt > 1:141193/1‑136 (MQ=255) gtgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGATTGCATTCCCCCCTTTTTTCATAATGatttc > 2:488130/1‑138 (MQ=255) tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTGGAAGGACCCCTTTTTTCAATAATGtttttg > 2:907804/1‑137 (MQ=255) tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGAATGCCCCCCTTTTTCAATAAATTTTgtt > 1:1065918/1‑138 (MQ=255) tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGCGGGTGGCAGGTCCCCCTTTTTCAATAATGtttttg > 2:856944/1‑137 (MQ=255) cTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGcc < 2:792027/140‑1 (MQ=255) aaaaaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTc < 1:608756/140‑1 (MQ=255) cAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGTATGCCCCCCTTTTTTAATAATTTTTTGGCCTcgccccg > 2:942110/1‑136 (MQ=255) ggTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGAATGCCCCCCTTTTTTAATAATTTTTTCGCCCCCCGCCGtg > 2:943257/1‑139 (MQ=255) ggTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGAATGCCCCCCTTTTTCAATAATGTTTTCGCCTCCCGCTGtt > 2:92881/1‑139 (MQ=255) tAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGc < 2:891847/140‑1 (MQ=255) tAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGc < 1:450506/140‑1 (MQ=255) tCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGGTGGATGCCCCCCTTTTTGGATAATTTTTGTTCCTCCCGCCGTGGGCCgcgc > 2:1002868/1‑140 (MQ=255) cAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCa < 2:100122/140‑1 (MQ=255) tAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTTGGATGCCCCCCTTTTTTTATAATGTTTTCGCCCCGCGCTGTAGCCAGCGCCGGc > 2:947780/1‑140 (MQ=255) gTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGcccc < 1:509294/140‑1 (MQ=255) cACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTTGGATGTCCCCCTTTTTTAACAAAATTTTTGCCTCCCGCCGTAGGCAGCGCAGGccccccccc > 2:558549/1‑137 (MQ=255) aCTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGc < 2:711376/140‑1 (MQ=255) ccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgcg < 1:244278/140‑1 (MQ=255) ccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgcg < 1:856944/140‑1 (MQ=255) tGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGGATGCCCCCCTTTTTCAATAATGTTTTTGCCTCCCCCCGTAGCCCGCGCCGGCCCCCCCCCCCCCCCCCCGCTGCa > 2:819207/1‑140 (MQ=255) ttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTAt < 1:371306/140‑1 (MQ=255) cgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCg < 1:960546/140‑1 (MQ=255) acacTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGt < 1:943257/140‑1 (MQ=255) tCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGAt < 1:666439/140‑1 (MQ=255) cTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGAATGCCCCCCTTTTTCTATAATATTTTCGCCTCCCGCCGTAGCCAGCGCAGGCCCCCCCCCCCCCCCCCCCCTGCAACCTTGGGATTGCCGTTGATCGc > 2:231743/1‑140 (MQ=255) tGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGcc < 2:695149/140‑1 (MQ=255) tGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGcc < 1:285070/140‑1 (MQ=255) tGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGcc < 1:947780/140‑1 (MQ=255) gTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCa < 2:739787/140‑1 (MQ=255) gTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGGTGGCAAGCCCCCCTTTTTCCATAATAATTTCGCCCCCCGCTTGAGCCCACGCAGGGCCCCCCCCCCCCCCCGCGCTGCCACGCTGGTATTGCCGGTGGACGccc > 2:342857/1‑139 (MQ=255) tCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCAt < 2:552989/140‑1 (MQ=255) aaCTGCCGGAGATAATCGAGATCGGC‑GGGGGGTCGGATGCCCCCCTTTTTCCATAATGTTTTCGCCCCCCGCCGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCCCGGTGCGACGCTGGGATCGCCCGTGATCGCCCTcca > 2:762042/1‑138 (MQ=255) caggCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGt < 1:533861/137‑1 (MQ=255) aCTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGTCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGt < 1:942110/140‑1 (MQ=255) aCTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGt < 1:44136/140‑1 (MQ=255) tGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAg < 1:488130/140‑1 (MQ=255) ccGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGa < 1:607491/140‑1 (MQ=255) gagaTAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCa < 1:838049/140‑1 (MQ=255) gagaTAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCa < 1:183027/140‑1 (MQ=255) aTCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTAt < 2:1065918/140‑1 (MQ=255) gaTCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGc < 1:342857/140‑1 (MQ=255) gc‑ggGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGc < 1:819207/140‑1 (MQ=255) gc‑ggGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGc < 1:1002868/140‑1 (MQ=255) c‑ggGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGcc < 2:141193/140‑1 (MQ=255) ggTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGtt < 1:907804/140‑1 (MQ=255) | AAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTT > NC_000913/646608‑646884 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |