Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,251,130	A→G	pseudogene (428/1581 nt)	yjbI →	pentapeptide repeats‑containing protein YjbI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,251,130	0	A	G	100.0%	311.4 / NA	82	pseudogene (428/1581 nt)	yjbI	pentapeptide repeats‑containing protein YjbI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/43);  total (39/43)

TAAAGATGCGCAGGGGTATATCGACCTGTCTGATTTGGATTTAACAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAGTGGTGAATGTGTCGATTTCAGCAAAAATATTCTTGATACAGTTGACTTCTCG  >  NC_000913/4250993‑4251263                                                                                                                                          |                                                                                                                                      tAAAGATGCGCAGGGGTATATCGACCTGTCTGATTTGGATTTAACAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGtt                                                                                                                                     >  2:534718/1‑140 (MQ=255)   aaGATGCGCAGGGGTATATCGACCTGTCTGATTTGGATTTAACAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGc                                                                                                                                   <  2:1020004/140‑1 (MQ=255)   aaGATGCGCAGGGGTATATCGACCTGTCTGATTTGGATTTAACAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGc                                                                                                                                   <  1:166053/140‑1 (MQ=255)   aaGATGCGCAGGGGTATATCGACCTGTCTGATTTGGATTTAACAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGc                                                                                                                                   <  1:55889/140‑1 (MQ=255)        gcgcAGGGGCATATCGACCTGTCTGATTTGGATTTAACAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAAttt                                                                                                                              <  2:895211/140‑1 (MQ=255)           cAGGGGTATATCGACCTGTCTGATTTGGATTTAACAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTtac                                                                                                                           <  2:15241/140‑1 (MQ=255)           cAGGGGTATATCGACCTGTCTGATTTGGATTTAACAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTtac                                                                                                                           <  1:547408/140‑1 (MQ=255)                gTATATCGACCTGTCTGATTTGGATTTAACAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTg                                                                                                                      <  1:928158/140‑1 (MQ=255)                gTATATCGACCTGTCTGATTTGGATTTAACAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTg                                                                                                                      <  1:127401/140‑1 (MQ=255)                  atatCGACCTGTCTGATTTGGATTTAACAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCa                                                                                                                    <  2:692670/140‑1 (MQ=255)                     tCGACCTGTCTGATTTGGATTTAACAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATa                                                                                                                 <  2:733506/140‑1 (MQ=255)                      cGACCTGTCTGATTTGGATTTAACAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAg                                                                                                                >  1:16053/1‑140 (MQ=255)                        aCCTGTCTGATTTGGATTTAACAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGtt                                                                                                              >  2:799906/1‑140 (MQ=255)                        aCCTGTCTGATTTGGATTTAACAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGtt                                                                                                              >  1:570847/1‑140 (MQ=255)                        aCCTGTCTGATTTGGATTTAACAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGtt                                                                                                              >  2:843177/1‑140 (MQ=255)                         ccTGTCTGATTTGGATTTAACAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTg                                                                                                             >  1:600149/1‑140 (MQ=255)                           tGTCTGATTTGGATTTAACAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGAt                                                                                                           >  2:854048/1‑140 (MQ=255)                            gTCTGATTTGGATTTAACAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATa                                                                                                          <  2:386077/140‑1 (MQ=255)                            gTCTGATTTGGATTTAACAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATa                                                                                                          <  1:1068768/140‑1 (MQ=255)                             tCTGATTTGGATTTAACAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATaa                                                                                                         >  1:628475/1‑140 (MQ=255)                               tGATTTGGATTTAACAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATg                                                                                                       <  2:628475/140‑1 (MQ=255)                                gATTTGGATTTAACAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGt                                                                                                      <  1:801451/140‑1 (MQ=255)                                 aTTTGGATTTAACAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTc                                                                                                     <  2:1008493/140‑1 (MQ=255)                                       aTTTAACAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATAttt                                                                                               >  2:293749/1‑140 (MQ=255)                                       aTTTAACAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATAttt                                                                                               >  1:34410/1‑140 (MQ=255)                                           aaCAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGAt                                                                                           >  2:684708/1‑140 (MQ=255)                                                gTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGa                                                                                      >  1:490317/1‑140 (MQ=255)                                                gTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGa                                                                                      >  1:423651/1‑140 (MQ=255)                                                gTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGa                                                                                      >  1:530927/1‑140 (MQ=255)                                                 ttGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGAc                                                                                     <  2:490317/140‑1 (MQ=255)                                                   gTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGGAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTc                                                                                        >  2:425207/1‑135 (MQ=255)                                                   gTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGGAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTc                                                                                        <  1:425207/135‑1 (MQ=255)                                                      aTTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTa                                                                                <  2:418261/140‑1 (MQ=255)                                                      aTTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTa                                                                                <  1:854048/140‑1 (MQ=255)                                                       ttttAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTac                                                                               <  2:600149/140‑1 (MQ=255)                                                        tttAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTaca                                                                              >  1:613925/1‑140 (MQ=255)                                                        tttAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTaca                                                                              <  2:570847/140‑1 (MQ=255)                                                             aGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCAAATTTAGATGTCGACGTTTACACAAtg                                                                         <  1:1012689/140‑1 (MQ=255)                                                                  aCGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGAtt                                                                    >  1:743979/1‑140 (MQ=255)                                                                         atCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTCGATGTCGACGTTTGCACAATGTGATTTTTATCa                                                             >  1:558347/1‑140 (MQ=255)                                                                         atCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAACGTGATTTTTATCa                                                             >  1:111704/1‑140 (MQ=255)                                                                          tCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCaa                                                            <  2:530927/140‑1 (MQ=255)                                                                          tCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCaa                                                            >  1:494562/1‑140 (MQ=255)                                                                            gAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAg                                                          <  2:974212/140‑1 (MQ=255)                                                                            gAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAg                                                          <  1:843177/140‑1 (MQ=255)                                                                            gAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAg                                                          <  1:293749/140‑1 (MQ=255)                                                                             aaGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGc                                                         <  1:931258/140‑1 (MQ=255)                                                                             aaGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGc                                                         <  1:121841/140‑1 (MQ=255)                                                                             aaGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGc                                                         >  2:931258/1‑140 (MQ=255)                                                                             aaGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGc                                                         >  1:585813/1‑140 (MQ=255)                                                                             aaGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGc                                                         <  1:367017/140‑1 (MQ=255)                                                                             aaGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGc                                                         >  2:408217/1‑140 (MQ=255)                                                                              aGGTGTCTTTTTTATCATCAAATTTTCAACATGTAACATTTGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCg                                                        <  1:534718/140‑1 (MQ=255)                                                                              aGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCg                                                        >  2:999889/1‑140 (MQ=255)                                                                              aGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCg                                                        >  2:242795/1‑140 (MQ=255)                                                                               ggTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGa                                                       >  1:108872/1‑140 (MQ=255)                                                                                gtgtCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAg                                                      <  1:95783/140‑1 (MQ=255)                                                                                   tCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAgtgg                                                   >  2:427987/1‑140 (MQ=255)                                                                                      tttttATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAGTGGTGa                                                <  2:312116/140‑1 (MQ=255)                                                                                        tttATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAGTGGTGAAt                                              <  2:400804/140‑1 (MQ=255)                                                                                                   aTCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAGTGGTGAATGTGTCGATTTc                                   <  2:585813/140‑1 (MQ=255)                                                                                                            aTGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAGTGGTGAATGTGTCGATTTCAGCAAAAat                          <  1:120864/140‑1 (MQ=255)                                                                                                            aTGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAGTGGTGAATGTGTCGATTTCAGCAAAAat                          <  1:799906/140‑1 (MQ=255)                                                                                                             tGTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAGTGGTGAATGTGTCGATTTCAGCAAAAata                         >  1:317293/1‑140 (MQ=255)                                                                                                              gTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAGTGGTGAATGTGTCGATTTCAGCAAAAatat                        >  1:798956/1‑140 (MQ=255)                                                                                                              gTAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAGTGGTGAATGTGTCGATTTCAGCAAAAatat                        >  2:320890/1‑140 (MQ=255)                                                                                                               tAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAGTGGTGAATGTGTCGATTTCAGCAAAAATAtt                       <  2:16053/140‑1 (MQ=255)                                                                                                               tAACATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAGTGGTGAATGTGTCGATTTCAGCAAAAATAtt                       >  1:714191/1‑140 (MQ=255)                                                                                                                 aCATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAGTGGTGAATGTGTCGATTTCAGCAAAAATATTCt                     <  2:743979/140‑1 (MQ=255)                                                                                                                 aCATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAGTGGTGAATGTGTCGATTTCAGCAAAAATATTCt                     <  2:714191/140‑1 (MQ=255)                                                                                                                  cATTCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAGTGGTGAATGTGTCGATTTCAGCAAAAATATTCtt                    >  1:964796/1‑140 (MQ=255)                                                                                                                    ttCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAGTGGTGAATGTGTCGATTTCAGCAAAAATATTCTTGa                  <  2:924516/140‑1 (MQ=255)                                                                                                                     tCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAGTGGTGAATGTGTCGATTTCAGCAAAAATATTCTTGAt                 >  2:281422/1‑140 (MQ=255)                                                                                                                     tCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAGTGGTGAATGTGTCGATTTCAGCAAAAATATTCTTGAt                 >  2:42876/1‑140 (MQ=255)                                                                                                                     tCGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAGTGGTGAATGTGTCGATTTCAGCAAAAATATTCTTGAt                 <  1:999889/140‑1 (MQ=255)                                                                                                                      cGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAGTGGTGAATGTGTCGATTTCAGCAAAAATATTCTTGATa                >  1:609579/1‑140 (MQ=255)                                                                                                                      cGAATGTAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAGTGGTGAATGTGTCGATTTCAGCAAAAATATTCTTGATa                >  1:240131/1‑140 (MQ=255)                                                                                                                            tAAAGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAGTGGTGAATGTGTCGATTTCAGCAAAAATATTCTTGATACAGTTg          >  2:194962/1‑140 (MQ=255)                                                                                                                              aaGAAATTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAGTGGTGAATGTGTCGATTTCAGCAAAAATATTCTTGATACAGTTGAc        >  1:82942/1‑140 (MQ=255)                                                                                                                                   aTTGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAGTGGTGAATGTGTCGATTTCAGCAAAAATATTCTTGATACAGTTGACTtctc   <  2:108872/140‑1 (MQ=255)                                                                                                                                   aTTGGGGGTTACAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAGTGGTGAATGTGTCGATTTCAGCAAAAATATTCTTGATACAGTTGACTtctc   >  1:465940/1‑140 (MQ=255)                                                                                                                                    ttGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAGTGGTGAATGTGTCGATTTCAGCAAAAATATTCTTGATACAGTTGACTTCTCg  <  1:281422/140‑1 (MQ=255)                                                                                                                                    ttGGGGGTTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAGTGGTGAATGTGTCGATTTCAGCAAAAATATTCTTGATACAGTTGACTTCTCg  <  2:609579/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      TAAAGATGCGCAGGGGTATATCGACCTGTCTGATTTGGATTTAACAAGTTGTCATTTTAAAGGTGACGTTATATCGAAGGTGTCTTTTTTATCATCAAATCTACAACATGTAACATTCGAATGTAAAGAAATTGGGGATTGCAATTTTACTACTGCAATAGTTGATAATGTCATATTTAGATGTCGACGTTTACACAATGTGATTTTTATCAAAGCGAGTGGTGAATGTGTCGATTTCAGCAAAAATATTCTTGATACAGTTGACTTCTCG  >  NC_000913/4250993‑4251263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑