Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913372,6400TG23.0% 150.9 / 14.7 74L176V (TTA→GTA) mhpD2‑hydroxypentadienoate hydratase
Reads supporting (aligned to +/- strand):  ref base T (22/35);  new base G (17/0);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 2.42e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CATTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCT  >  NC_000913/372501‑372779
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cATTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGt                                                                                                                                             <  1:866101/140‑1 (MQ=255)
  ttGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCCACGTCCGGCGGGGGTa                                                                                                                                           >  1:1051671/1‑140 (MQ=255)
  ttGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTa                                                                                                                                           >  1:1029593/1‑140 (MQ=255)
  ttGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTa                                                                                                                                           >  2:556337/1‑140 (MQ=255)
   tGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGAGTGGTCGATTCAGTTGGTCGATACCGTGGCAGATAACGCCTCGTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAg                                                                                                                                          <  2:914109/140‑1 (MQ=255)
   tGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAg                                                                                                                                          <  2:1221254/140‑1 (MQ=255)
       tGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCt                                                                                                                                      >  1:918079/1‑140 (MQ=255)
          gTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGaa                                                                                                                                   >  1:701795/1‑140 (MQ=255)
             cTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGaaaaa                                                                                                                                <  2:698694/140‑1 (MQ=255)
             cTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAAACCTGaaaaa                                                                                                                                <  2:267548/140‑1 (MQ=255)
               tCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGGCCGGCGGGGGTAGACCTGCAAAACt                                                                                                                              >  1:1183287/1‑140 (MQ=255)
                ccGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTg                                                                                                                             <  2:1496615/140‑1 (MQ=255)
                ccGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTg                                                                                                                             >  2:64562/1‑140 (MQ=255)
                  ggCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGACGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTgcg                                                                                                                           <  2:779525/140‑1 (MQ=255)
                   gcgcTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCGACGTCCGGCGGGGGTAGACCTGAAAAAATgcgg                                                                                                                          >  1:1140648/1‑139 (MQ=255)
                   gcgcTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTgcgc                                                                                                                          <  2:918079/140‑1 (MQ=255)
                   gcgcTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTgcgc                                                                                                                          >  2:385769/1‑140 (MQ=255)
                   gcgcTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTgcgc                                                                                                                          >  1:538785/1‑140 (MQ=255)
                    cgcTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACAGCGcc                                                                                                                         >  1:695615/1‑140 (MQ=255)
                    cgcTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGGCCGGCGGGGGTAGACCTTAAAAAATGCGcc                                                                                                                         >  2:1444970/1‑140 (MQ=255)
                           aGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGa                                                                                                                  <  2:1293970/140‑1 (MQ=255)
                           aGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCCGCGGGGGTAGACCCGTAAAACTTCGCCacgtaaa                                                                                                                  >  1:551859/1‑134 (MQ=255)
                                tggGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGAcg                                                                                                             <  2:526730/140‑1 (MQ=255)
                                tggGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGAcg                                                                                                             <  1:364198/140‑1 (MQ=255)
                                 ggggAGCCGTATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGAcgc                                                                                                            >  2:570599/1‑140 (MQ=255)
                                 ggggAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGAcgc                                                                                                            >  2:1444111/1‑140 (MQ=255)
                                  gggAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGAcgcg                                                                                                           <  1:556337/140‑1 (MQ=255)
                                     aGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGtaa                                                                                                        >  1:536826/1‑140 (MQ=255)
                                       ccGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGtaata                                                                                                      <  1:1444111/140‑1 (MQ=255)
                                       ccGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGtaata                                                                                                      >  1:920715/1‑140 (MQ=255)
                                       ccGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCCGCGGGGTTAGACCTGAAAAACTGCGCCCTGAAGATGACGCGgaata                                                                                                      >  2:455735/1‑140 (MQ=255)
                                            ttCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGaa                                                                                                 <  1:362586/140‑1 (MQ=255)
                                            ttCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGaa                                                                                                 <  2:1029593/140‑1 (MQ=255)
                                              cgcgACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAaga                                                                                               <  2:901547/140‑1 (MQ=255)
                                                      gTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTa                                                                                       <  1:1058674/140‑1 (MQ=255)
                                                         gATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCCACGTCCGGCGGGGGTAGACCTGAAAAAACGCGCCCTGAAGATGACGCGGAATAACGGAGAGGGTTTTAACg                                                                                    >  2:747659/1‑140 (MQ=255)
                                                         gATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCCTGAAGATGGCGCGGAATAAAGAAGAGGGTTCTAGCg                                                                                    >  2:211175/1‑140 (MQ=255)
                                                         gATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCg                                                                                    >  2:547131/1‑140 (MQ=255)
                                                                tttGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCgg                                                                             <  2:538785/140‑1 (MQ=255)
                                                                     cGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCg                                                                        <  2:701795/140‑1 (MQ=255)
                                                                     cGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCg                                                                        >  2:955077/1‑140 (MQ=255)
                                                                     cGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCg                                                                        <  1:793125/140‑1 (MQ=255)
                                                                      gATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGa                                                                       <  2:490582/140‑1 (MQ=255)
                                                                       aTACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGaa                                                                      <  1:1114072/140‑1 (MQ=255)
                                                                              ggCAGATAACGCCTCCTGTTGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTTTAGCGGGCGCGGCAGCGAAAGCCTgg                                                               >  2:253245/1‑140 (MQ=255)
                                                                              ggCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATGCCTgg                                                               >  1:885617/1‑140 (MQ=255)
                                                                              ggCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTgg                                                               >  2:1422605/1‑140 (MQ=255)
                                                                                  gATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACa                                                           <  2:695615/140‑1 (MQ=255)
                                                                                    tAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCCTGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAAAGGCTGGGACATc                                                         >  2:99494/1‑140 (MQ=255)
                                                                                          ctcctGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTa                                                   >  2:678997/1‑140 (MQ=255)
                                                                                           tcctGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTaa                                                  >  1:1313347/1‑140 (MQ=255)
                                                                                             ctGTTGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATg                                                <  1:253245/140‑1 (MQ=255)
                                                                                             ctGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAAAGGAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATg                                                >  2:423391/1‑140 (MQ=255)
                                                                                                tgGGGTGTATGTCACCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCgg                                             <  2:551859/140‑1 (MQ=255)
                                                                                                 ggggTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCCTGAAGAAGACGCGTAATAACGAAGAGGGTTCTAACGGGCCCGGCAGCGAATGCCTGGGACAACCCCCTAATGCGGc                                            >  2:1385799/1‑140 (MQ=255)
                                                                                                      gtATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCCTGAAGATGACGCGTAATAAAGAAGAGGGTTCTAAGGGGCGCGGGAGCGAATGCCTGGGGCATCCGCTTAATGCGGCCGGct                                       >  2:1101547/1‑140 (MQ=255)
                                                                                                       tATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCg                                                       <  1:483492/123‑1 (MQ=255)
                                                                                                       tATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCg                                                       >  2:483492/1‑123 (MQ=255)
                                                                                                       tATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTctg                                      >  1:1175159/1‑140 (MQ=255)
                                                                                                       tATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTTGACCTGAAAAACTGCGCCATGAAGATGACGCGGAATAACGAAGAGGGTTCTAGCGGGGGCGGCAGCGGATGCCTGGGGCATCCGCTTAATGCGGGCGGctg                                      >  2:490751/1‑140 (MQ=255)
                                                                                                       tATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTctg                                      >  1:734541/1‑140 (MQ=255)
                                                                                                       tATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTctg                                      >  1:573031/1‑140 (MQ=255)
                                                                                                       tATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAAATGCGCCATGAAGATGACGCGTAATAAAGAAGAGGGTTCTAGCGGGCGCGGCAGCGAAAGCCTGGGGCATCCGCCTAATGCGGCCGTctg                                      >  2:337298/1‑140 (MQ=255)
                                                                                                        aTGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTctgg                                     <  1:99494/140‑1 (MQ=255)
                                                                                                          gTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTctggct                                   >  1:833812/1‑140 (MQ=255)
                                                                                                            cATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTctggctgg                                 >  1:45477/1‑140 (MQ=255)
                                                                                                             aTCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCtggctggc                                >  1:220519/1‑140 (MQ=255)
                                                                                                             aTCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCtggctggc                                <  1:455735/140‑1 (MQ=255)
                                                                                                             aTCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCtggctggc                                >  1:1320506/1‑140 (MQ=255)
                                                                                                              tCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCa                               <  1:385769/140‑1 (MQ=255)
                                                                                                                 gcggTCCGGCGCAACGTCCGGGGGGGTTAGACCTGAAAAACTGCGCCATGAAGGTGACGCGTAATAACGAAAAGGGTTTTAGCGGGCGCGGCAGCGAAAGCCCGGGGCATCCGCTTAATGCGGGCGTCTGGCTGGCCCGc                            >  2:1316615/1‑140 (MQ=255)
                                                                                                                 gcggTCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGc                            >  2:633866/1‑140 (MQ=255)
                                                                                                                  cggTCCCGCGCAACGTCCCGCGGGGTTAGAACCGAAAAACTGCGCCATGAAGATGACGCGTAAAAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATTCCTGGGACATCCGCTTTATGCGGGCGGCTGGCTGGCACGCa                           >  2:45882/1‑140 (MQ=255)
                                                                                                                   ggTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCaa                          <  2:920715/140‑1 (MQ=255)
                                                                                                                    gTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCaaa                         <  2:1255256/140‑1 (MQ=255)
                                                                                                                     tCCGGCGCAACGTCCGGCGGGGTTAGGCCTGAAAAACTGCGGCCTGAAGATGGCGCGTAATAAAGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATGCCTGGGGCATCCGCTTAATGCGGCCCGCTGGCTGGCACCCaaaa                        >  2:725064/1‑140 (MQ=255)
                                                                                                                     tCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGATGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGGCATCCGCTTAATGCGGCCGTCTGGCTGGCACGCaaaa                        >  2:1357978/1‑140 (MQ=255)
                                                                                                                     tCCGGCGCAACGTCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCaaaa                        >  2:847675/1‑140 (MQ=255)
                                                                                                                         gcgcAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGTACGCAAAATGGc                    <  2:1051671/140‑1 (MQ=255)
                                                                                                                         gcgcAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGc                    <  1:64562/140‑1 (MQ=255)
                                                                                                                          cgcAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGcc                   >  2:358030/1‑140 (MQ=255)
                                                                                                                           gcAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCa                  >  2:823899/1‑140 (MQ=255)
                                                                                                                             aaCGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGt                <  2:660520/140‑1 (MQ=255)
                                                                                                                             aaCGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGt                <  1:211175/140‑1 (MQ=255)
                                                                                                                             aaCGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGt                <  1:1444970/140‑1 (MQ=255)
                                                                                                                               cGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCt              >  2:50382/1‑140 (MQ=255)
                                                                                                                                       ggggTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAAc      >  1:1024617/1‑140 (MQ=255)
                                                                                                                                          gTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGc   >  2:715835/1‑140 (MQ=255)
                                                                                                                                           ttAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCt  <  1:633866/140‑1 (MQ=255)
                                                                                                                                           ttAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCt  <  2:45477/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CATTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCT  >  NC_000913/372501‑372779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑