Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	175,372	T→G	L89R (CTG→CGG)	clcA →	chloride:H(+) antiporter ClcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	175,372	0	T	G	100.0%	347.0 / NA	92	L89R (CTG→CGG)	clcA	chloride:H(+) antiporter ClcA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (55/37);  total (55/37)

GCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGC  >  NC_000913/175238‑175508                                                                                                                                       |                                                                                                                                         gCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGa                                                                                                                                     <  2:1025608/140‑1 (MQ=255) gCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGa                                                                                                                                     <  2:738053/140‑1 (MQ=255)        gggCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTgg                                                                                                                              >  1:392980/1‑140 (MQ=255)        gggCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTgg                                                                                                                              <  2:362157/140‑1 (MQ=255)        gggCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTgg                                                                                                                              <  2:862462/140‑1 (MQ=255)        gggCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTgg                                                                                                                              <  1:1181315/140‑1 (MQ=255)           cTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTa                                                                                                                           >  1:1138478/1‑140 (MQ=255)             ggCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGa                                                                                                                                     <  1:656205/128‑1 (MQ=255)             ggCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGa                                                                                                                                     >  2:656205/1‑128 (MQ=255)             ggCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACt                                                                                                                         >  1:875247/1‑140 (MQ=255)             ggCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGATAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACt                                                                                                                         >  1:506984/1‑140 (MQ=255)               cAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACttt                                                                                                                       >  1:1123104/1‑140 (MQ=255)                        cTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTgcgc                                                                                                              >  1:1177481/1‑140 (MQ=255)                        cTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTgcgc                                                                                                              >  1:1259346/1‑140 (MQ=255)                              aCAAAGGTGTCGCCTGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATAc                                                                                                        <  1:714917/140‑1 (MQ=255)                              aCAAAGGTGTCGCCTGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATAc                                                                                                        <  2:506391/140‑1 (MQ=255)                                       tCGCCTGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGaa                                                                                               >  2:1028892/1‑140 (MQ=255)                                            tGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAgg                                                                                          <  2:1138478/140‑1 (MQ=255)                                            tGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAgg                                                                                          <  1:328155/140‑1 (MQ=255)                                              gTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACgcgc                                                                                                    >  2:235851/1‑128 (MQ=255)                                              gTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACgcgc                                                                                                    <  1:235851/128‑1 (MQ=255)                                              gTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAggtg                                                                                        >  2:1231578/1‑140 (MQ=255)                                                  cAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTc                                                                                    >  1:604626/1‑140 (MQ=255)                                                       ccAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGa                                                                               >  2:1064096/1‑140 (MQ=255)                                                         aaCGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATc                                                                             >  1:924255/1‑140 (MQ=255)                                                          aCGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATcc                                                                            >  2:884167/1‑140 (MQ=255)                                                            gTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCg                                                                          <  2:1123104/140‑1 (MQ=255)                                                            gTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGATAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCg                                                                          <  2:506984/140‑1 (MQ=255)                                                                   ggCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTg                                                                   >  2:132980/1‑140 (MQ=255)                                                                    gcgcTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGa                                                                  >  1:557916/1‑140 (MQ=255)                                                                       cTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAgg                                                               >  1:961757/1‑140 (MQ=255)                                                                         ggTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAgggg                                                             <  1:100741/140‑1 (MQ=255)                                                                            aCATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGgcgc                                                          <  2:1333693/140‑1 (MQ=255)                                                                               tACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTgg                                                       <  2:1259346/140‑1 (MQ=255)                                                                               tACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTgg                                                       <  1:132980/140‑1 (MQ=255)                                                                                 ctgctgATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGaa                                                     <  2:1387577/140‑1 (MQ=255)                                                                                 ctgctgATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGaa                                                     <  2:940840/140‑1 (MQ=255)                                                                                 ctgctgATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGaa                                                     <  2:392980/140‑1 (MQ=255)                                                                                  tgctgATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAg                                                    >  2:1220126/1‑140 (MQ=255)                                                                                    ctgATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGTTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGAt                                                  >  1:724084/1‑140 (MQ=255)                                                                                    ctgATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGAt                                                  >  1:1466472/1‑140 (MQ=255)                                                                                    ctgATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGAt                                                  >  1:692799/1‑140 (MQ=255)                                                                                    ctgATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGAt                                                  >  1:525652/1‑140 (MQ=255)                                                                                    ctgATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGAt                                                  >  1:1460630/1‑140 (MQ=255)                                                                                    ctgATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGAt                                                  >  2:1459753/1‑140 (MQ=255)                                                                                    ctgATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGAt                                                  >  1:1334130/1‑140 (MQ=255)                                                                                    ctgATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGAt                                                  >  1:1273452/1‑140 (MQ=255)                                                                                    ctgATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGAt                                                  >  1:1216091/1‑140 (MQ=255)                                                                                    ctgATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGAt                                                  >  2:528726/1‑140 (MQ=255)                                                                                    ctgATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGAt                                                  >  1:1120856/1‑140 (MQ=255)                                                                                    ctgATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGAt                                                  >  1:1009916/1‑140 (MQ=255)                                                                                    ctgATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTATGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGAt                                                  >  1:832760/1‑140 (MQ=255)                                                                                     tgATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATc                                                 <  2:1120856/140‑1 (MQ=255)                                                                                       aTAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTCCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCaa                                               >  2:80444/1‑140 (MQ=255)                                                                                         aaTTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACg                                             >  2:379537/1‑140 (MQ=255)                                                                                           ttATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTc                                           >  2:503983/1‑140 (MQ=255)                                                                                              tCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGTTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCg                                        <  2:724084/140‑1 (MQ=255)                                                                                              tCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCg                                        >  2:909261/1‑140 (MQ=255)                                                                                              tCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCg                                        >  1:1333390/1‑140 (MQ=255)                                                                                              tCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCg                                        >  1:169261/1‑140 (MQ=255)                                                                                              tCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCg                                        >  1:226402/1‑140 (MQ=255)                                                                                              tCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCg                                        <  1:1064096/140‑1 (MQ=255)                                                                                                cGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGtt                                      <  2:875247/140‑1 (MQ=255)                                                                                                   ttCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGc                                   >  1:1105824/1‑140 (MQ=255)                                                                                                   ttCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGc                                   >  2:1206925/1‑140 (MQ=255)                                                                                                     cTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCtg                                 <  1:1220126/140‑1 (MQ=255)                                                                                                      tGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCg                                    >  1:36967/1‑136 (MQ=255)                                                                                                      tGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCg                                    <  2:36967/136‑1 (MQ=255)                                                                                                        ttAACCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCtggtg                              <  1:1206925/140‑1 (MQ=255)                                                                                                          aaCCGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGc                            >  1:1073486/1‑140 (MQ=255)                                                                                                             cGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCgtg                         <  2:961757/140‑1 (MQ=255)                                                                                                             cGTCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCgtg                         >  2:1292705/1‑140 (MQ=255)                                                                                                               tCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTa                       >  1:740556/1‑140 (MQ=255)                                                                                                               tCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTa                       >  1:457246/1‑140 (MQ=255)                                                                                                               tCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTa                       >  2:1272153/1‑140 (MQ=255)                                                                                                               tCGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGGTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGCTGGCGTGTa                       >  2:117343/1‑140 (MQ=255)                                                                                                                cGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGGAGATCAACGTCCCGTTCGCTGGTGGCGTGTAt                      >  1:1100276/1‑140 (MQ=255)                                                                                                                cGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTGGCTGGTGGCGTGTAt                      >  2:1313381/1‑140 (MQ=255)                                                                                                                cGCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTAt                      >  2:1479586/1‑140 (MQ=255)                                                                                                                cGCTTTTCTCTGCTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTAt                      >  1:1312335/1‑140 (MQ=255)                                                                                                                 gCTTTTCTCTGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTAtt                     <  1:479540/140‑1 (MQ=255)                                                                                                                       ctctGTTCGGCGGTGCGGGCGATGTTTGGTTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGt               >  2:84825/1‑140 (MQ=255)                                                                                                                        tctGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTg              <  1:1231578/140‑1 (MQ=255)                                                                                                                        tctGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTg              <  1:1292705/140‑1 (MQ=255)                                                                                                                        tctGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTg              <  2:557916/140‑1 (MQ=255)                                                                                                                        tctGTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTg              <  2:1177481/140‑1 (MQ=255)                                                                                                                           gTTCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAg           <  2:1333390/140‑1 (MQ=255)                                                                                                                            ttCGGCGGTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGt          <  1:503983/140‑1 (MQ=255)                                                                                                                               ggcggTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCt       >  1:1396571/1‑140 (MQ=255)                                                                                                                                  ggTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTg    <  1:884167/140‑1 (MQ=255)                                                                                                                                   gTGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTgg   <  1:1028892/140‑1 (MQ=255)                                                                                                                                    tGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGGGGCGTGTATTGCCGGTGAAGTTCTTTGGc  >  1:1419319/1‑140 (MQ=255)                                                                                                                                    tGCGGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCAGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGc  <  1:80444/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         GCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCCGGAAATTGAAGGGGCGCTGGAAGATCAACGTCCCGTTCGCTGGTGGCGTGTATTGCCGGTGAAGTTCTTTGGC  >  NC_000913/175238‑175508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑