Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	445,558	0	A	C	32.0%	74.9 / 30.9	50	V370G (GTA→GGA)	yajR	putative major facilitator superfamily transport protein YajR
Reads supporting (aligned to +/- strand):  ref base A (24/10);  new base C (0/16);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.23e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAA  >  NC_000913/445425‑445690                                                                                                                                      |                                                                                                                                     aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTTATGGTACTGGCGACTGTCAGCCACACTGCGGCCCGCATTTCGCCAGCGAGAAATAcccccc                                                                                                                                >  2:1170334/1‑139 (MQ=255)   gCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGa                                                                                                                              <  1:1598197/140‑1 (MQ=255)      cTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCg                                                                                                                           <  1:946920/140‑1 (MQ=255)        tttAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTc                                                                                                                         <  2:1673180/140‑1 (MQ=255)             cGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACa                                                                                                                    <  1:895915/140‑1 (MQ=255)             cGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGGTATTTTATGGTACATGCGACTGTCAGCCACACCGCCACCAGCCTTCCGCCCGCGAAAAACACCCCCCCACCCGCAAACa                                                                                                                    >  2:577874/1‑140 (MQ=255)             cGCCTCGTTTGCGGCAATGTCCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCGCACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACa                                                                                                                    <  1:1248691/140‑1 (MQ=255)              gCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGTCAGCGAGAAATACCCCCTGACCGTCAAACAt                                                                                                                   <  1:1461170/140‑1 (MQ=255)               ccTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCCTTGCGCCAGCGAGAAATACCCCCCGACCGTCAAACATg                                                                                                                  >  1:960026/1‑140 (MQ=255)                 tCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAAAACCCCCTGACCGTCAAACATGcc                                                                                                                >  2:83965/1‑140 (MQ=255)                 tCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGGTCTTTCATGGGACCGGCGGCCGTCAGCCACACTGCCGGCCGCCTTTCGCCAGCGAGGAAAAACCCCCGACCCGCCAACAAGcc                                                                                                                >  2:337005/1‑140 (MQ=255)                      tGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTaa                                                                                                           <  2:826423/140‑1 (MQ=255)                        cGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATc                                                                                                         <  1:1321673/140‑1 (MQ=255)                           cAATGTTCGCTGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTGCTGGCGACTGACAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAg                                                                                                      <  2:1242680/140‑1 (MQ=255)                           cAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAg                                                                                                      <  2:163991/140‑1 (MQ=255)                             aTGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAgcc                                                                                                    <  1:83965/140‑1 (MQ=255)                                  cGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTTTTTTATGGGACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCCGCGAGAAATACCCCCCGACCGTCAAACAAGCCGTTTATCCAGCCGcccc                                                                                               >  2:979701/1‑139 (MQ=255)                                  cGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGGACTGGCGACTGTCAGCCACACCGCGGCCCGCATTGCGCCAGCGGGAAATACCCCCCGACCGTCAAACATGCCGCTAATCCAGCCGcccc                                                                                               >  2:171166/1‑139 (MQ=255)                                                  cgcAAATTGTTGACATGGGGCGTTTTTTTCATGGTACTGCCGACTTTCCGCCACCCCGGGGCCCGCATGCCCCCAGCGAGAAATACCCCCTGACCGCCAACCATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgc                                                                               <  2:328232/140‑1 (MQ=255)                                                    cAAACTGCTGCCATCCGGCGTTTTTTTCATGGTACGGGCGACTGTCGCCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgcca                                                                             <  2:655740/140‑1 (MQ=255)                                                           ctgACATACGGCGTTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAg                                                                      <  1:1482280/140‑1 (MQ=255)                                                                   cggcggGTCTTTCCTGGTACAGGCGAGCGTCAGCCACACCTCCGCCATCAGTGCGTCCACCTGAAATATCCCCCGCCCCCCAAAAATGCCGTTAATCCAGCCGCCCAGCGTACCGCCCATCCCCACGCCGATaaaaacgg                                                              >  2:215743/1‑135 (MQ=255)                                                                      ggtttCTTTCAGGGTACTGGCGACTGTCAGCCACACGGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGACCTTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAActggctgg                                                           <  1:1096708/137‑1 (MQ=255)                                                                       gtttCTTTCATGGTACGGGGGACTGCCGGCCACACTGCGGCCAGCTTTGCGCCAGCAAAAAATCCCCCCTGCCCGTCAAACATGCCGTTAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggt                                                          <  2:129256/138‑1 (MQ=255)                                                                        tttttttCATGGTACGGGCGACTGTCAGCCACACGGCGGCAAGCATTGCGCCAGCGAGAAATCCCCCTGGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggtg                                                         <  2:1673085/136‑1 (MQ=255)                                                                        gTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggtg                                                         <  1:1408258/140‑1 (MQ=255)                                                                             ttaagggTACTGGCGACTGTCAGCCACACTGGGGCCAGCATTGCGCCGGCGAGAAATCCCCCCTGACCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTa                                                    <  1:1762148/135‑1 (MQ=255)                                                                                   gtccgggCAACTTTCACCCACACGGCGGCCAGCATTGCCCCAGCGAAAAAACCCCCCTACCCGCAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAcc                                              <  2:1518244/135‑1 (MQ=255)                                                                                    tACGGGCGACTGCCCGCCCCACGGCGGCCACCTTTGCGCGAGGGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAccc                                             <  2:156579/140‑1 (MQ=255)                                                                                     ccgggCGACTGTCAGCCACACGGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAACCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCa                                            <  1:337005/137‑1 (MQ=255)                                                                                      cTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCAt                                           >  1:840844/1‑140 (MQ=255)                                                                                         gCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGc                                        >  2:1051890/1‑140 (MQ=255)                                                                                             ctgctaGCCCCACAGCGGCGAGCATCGCAAGAGGGAAAAATGCGCGCTGCCCACAAAACATCGCGTTAATCCAGCCGCCCAGGGAGCCGCCAATCGCCCCGCCAAGAAACTGGCTGGTGGAGTAAACGCCCATCGCCGTa                                    <  2:1529992/135‑1 (MQ=255)                                                                                             cTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                    >  2:580078/1‑140 (MQ=255)                                                                                             cTGCCAGCCCCACTGCGGCCAGCATTGCGCCAGGGAAAAATCCCCCCGGACCGTAAAACATGCCGTTAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                    <  2:1268813/140‑1 (MQ=255)                                                                                              tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                   >  2:917253/1‑140 (MQ=255)                                                                                              tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGCGGAGTAAACACCCATCGCCGTAc                                   >  2:110321/1‑140 (MQ=255)                                                                                                tCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCt                                 >  1:13772/1‑140 (MQ=255)                                                                                                tCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATA‑CCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCtt                                >  1:917856/1‑140 (MQ=255)                                                                                                   gCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTg                              >  2:868082/1‑140 (MQ=255)                                                                                                        acTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAAcc                         >  2:1092652/1‑140 (MQ=255)                                                                                                          tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                       >  2:1687852/1‑140 (MQ=255)                                                                                                          ggCGGCCAGCTTTGCGCCAGCGAAAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                       <  2:960026/139‑1 (MQ=255)                                                                                                          ggCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                       <  2:509687/139‑1 (MQ=255)                                                                                                            cGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctgct                     >  1:1543523/1‑140 (MQ=255)                                                                                                            cGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctgct                     >  2:295826/1‑140 (MQ=255)                                                                                                              gCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                   <  1:1092652/140‑1 (MQ=255)                                                                                                              gCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                   <  1:1051890/140‑1 (MQ=255)                                                                                                              gCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                   >  2:226478/1‑140 (MQ=255)                                                                                                              gCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                   >  1:1177671/1‑140 (MQ=255)                                                                                                              gCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                   >  1:205391/1‑140 (MQ=255)                                                                                                                cAGAATTGCGCCAGCGAAAAAT‑CCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                <  2:917856/140‑1 (MQ=255)                                                                                                                 aGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                >  1:823975/1‑140 (MQ=255)                                                                                                                     ttGCGCCAGCGAGAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct            <  1:908086/140‑1 (MQ=255)                                                                                                                     ttGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct            <  1:29917/140‑1 (MQ=255)                                                                                                                      tGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCtt           >  1:785399/1‑140 (MQ=255)                                                                                                                       gcgcCAGGGAGAAATCCCCCTTGACCGCCAAACATGCAGTTAATCAGGCGGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt          <  2:1462623/140‑1 (MQ=255)                                                                                                                       gccccAGCGAAAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt          <  2:13772/137‑1 (MQ=255)                                                                                                                        cgcCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTa         >  1:306285/1‑140 (MQ=255)                                                                                                                        cgcCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTa         >  1:355200/1‑140 (MQ=255)                                                                                                                            aGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGa     <  1:295826/140‑1 (MQ=255)                                                                                                                            aGCGAGAAATCCCCCCTGACCGCAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGa     <  1:1529425/140‑1 (MQ=255)                                                                                                                             gCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt    >  1:1646753/1‑140 (MQ=255)                                                                                                                             gCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt    >  2:879256/1‑140 (MQ=255)                                                                                                                             gCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt    >  1:11737/1‑140 (MQ=255)                                                                                                                             gCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCACCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt    >  2:750301/1‑140 (MQ=255)                                                                                                                               gagaAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATaa  <  1:171166/140‑1 (MQ=255)                                                                                                                               aaaaaaTCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATaa  <  1:979701/137‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     ACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAA  >  NC_000913/445425‑445690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑