Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,782,5090AC20.4% 408.4 / 21.1 162V73G (GTA→GGA) ypjAadhesin‑like autotransporter YpjA
Reads supporting (aligned to +/- strand):  ref base A (85/44);  new base C (0/33);  total (85/77)
Fisher's exact test for biased strand distribution p-value = 2.21e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 174 total aligned reads displayed.

GTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACCACACCAATAACATTAGTAGTGAAGGCAGCCATCC  >  NC_000913/2782371‑2782647
                                                                                                                                          |                                                                                                                                          
    tACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCg                                                                                                                                       >  2:164455/1‑140 (MQ=255)
    tACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCg                                                                                                                                       >  2:61783/1‑140 (MQ=255)
     aCCTGTGGAAATCCCCCCGTCATAAATTAACTATTTCCCACCGTTAATGGTTTTATTATTATCCGCCCCCACAAAATTGTTATGACTTCCTATTTCTTAATACCCACCAAATTAAAAAACACTCCAATTAGATCCCCCGc                                                                                                                                      <  2:1291620/140‑1 (MQ=255)
        tgtgGAAATCCCCCCGCCATGAATTGACTGTCTTCCACCGTTAATGGTTGTATTATTAGCCTGCCCCCCAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATCCCCCGCCAt                                                                                                                                   <  1:1131285/140‑1 (MQ=255)
        tgtgGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCAt                                                                                                                                   <  2:1657317/140‑1 (MQ=255)
        tgtgAAAATCCCCCCGTCATGAATTGACTGTCTGCCACCTTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATTTCTTGATACCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCAt                                                                                                                                   <  2:362584/140‑1 (MQ=255)
          tgGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGa                                                                                                                                 >  1:232081/1‑140 (MQ=255)
           ggAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGaa                                                                                                                                >  1:1338625/1‑140 (MQ=255)
           ggAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGaa                                                                                                                                >  1:38134/1‑140 (MQ=255)
                 ccccccGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATCCCCCGCCATGAACATTct                                                                                                                          <  2:391968/139‑1 (MQ=255)
                 aCCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTct                                                                                                                          >  2:630805/1‑140 (MQ=255)
                  cccccGTCATGAATTGACTGTCTGCCACCGTTAATGTTTGTTTTATTAGCCTCCCCCACAAAATTTTTATGACTTCCTATATCTTGATATCCACCAGATTAAATAAGACTTCCATTAGATCCCCCGCCATGAACATTctg                                                                                                                         <  2:859889/140‑1 (MQ=255)
                  cccccGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTTTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTctg                                                                                                                         <  1:200719/140‑1 (MQ=255)
                  cccccGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATCCCCCGCCATGAACATTctg                                                                                                                         <  1:621567/140‑1 (MQ=255)
                  cccccGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTctg                                                                                                                         <  2:499681/140‑1 (MQ=255)
                    cccGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTctgct                                                                                                                       >  1:1233811/1‑140 (MQ=255)
                    cccGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGCTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTctgct                                                                                                                       >  2:280641/1‑140 (MQ=255)
                      cGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTgg                                                                                                                     >  1:1031638/1‑140 (MQ=255)
                        tCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGcc                                                                                                                   >  1:409487/1‑140 (MQ=255)
                        tCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGcc                                                                                                                   <  1:1344054/140‑1 (MQ=255)
                        tCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGcc                                                                                                                   >  2:562897/1‑140 (MQ=255)
                        tCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGcc                                                                                                                   <  1:198989/140‑1 (MQ=255)
                          aTGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTTTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCAt                                                                                                                 <  1:540524/140‑1 (MQ=255)
                          aTGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATCCCCCGCCATGAACATTCTGCTGGCCAt                                                                                                                 <  2:1203945/140‑1 (MQ=255)
                          aTGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCAt                                                                                                                 <  2:1338625/140‑1 (MQ=255)
                           tGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATg                                                                                                                >  2:1708320/1‑140 (MQ=255)
                           tGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATACCCACCAGATTCAATAAGACTTCCATTAGATCCCCCGCCATGAACATTCTGCTGGCCATg                                                                                                                <  1:885268/140‑1 (MQ=255)
                            gAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATgg                                                                                                               <  1:99724/140‑1 (MQ=255)
                             aaTTGACTTTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATCCCCCGCCATGAACATTCTGCTGGCCATGGt                                                                                                              <  2:1380040/140‑1 (MQ=255)
                              aTTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGtt                                                                                                             <  2:38134/140‑1 (MQ=255)
                              aTTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGtt                                                                                                             <  2:1463445/140‑1 (MQ=255)
                                  aCTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGata                                                                                                         >  1:494608/1‑140 (MQ=255)
                                      tgtacaaCCGTTAATGGTTGTTTTATTTCCCTGCCCCATAAAATTGTTATGACTTCCTATATCTTGATATCCTCCAAATTAAATAAGTTTCCTATAAGATCCCCCGCCATGAACATTCTGCTGGCCATGGTTGATAatat                                                                                                     <  2:339328/134‑1 (MQ=255)
                                      tCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAatat                                                                                                     <  2:158848/140‑1 (MQ=255)
                                      acagtcACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCTTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAatat                                                                                                     <  1:338925/135‑1 (MQ=255)
                                       cTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATg                                                                                                    >  2:734681/1‑140 (MQ=255)
                                       cTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATg                                                                                                    >  1:1633186/1‑140 (MQ=255)
                                        tGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGa                                                                                                   <  1:1708320/140‑1 (MQ=255)
                                        tGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGa                                                                                                   <  1:280585/140‑1 (MQ=255)
                                        cgCCACCGTTAATGGTTGTATTATTAGCCTGCCCCAAAAAATTTTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATCCCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGa                                                                                                   <  2:1756470/139‑1 (MQ=255)
                                         gCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAg                                                                                                  >  2:1107589/1‑140 (MQ=255)
                                          ccACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAgt                                                                                                 <  1:1488066/140‑1 (MQ=255)
                                             ccGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGtt                                                                                              <  2:1233811/140‑1 (MQ=255)
                                                ttAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTAtt                                                                                           <  1:164455/140‑1 (MQ=255)
                                                    tGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATttgtt                                                                                       >  1:54991/1‑140 (MQ=255)
                                                          tattatTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCt                                                                                 >  1:1383639/1‑140 (MQ=255)
                                                          tattatTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCt                                                                                 >  2:484396/1‑140 (MQ=255)
                                                               ttaGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTc                                                                            >  2:230871/1‑140 (MQ=255)
                                                                 aGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCTTGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCAt                                                                          >  1:813897/1‑140 (MQ=255)
                                                                 aGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCAt                                                                          >  2:916624/1‑140 (MQ=255)
                                                                  gCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATCCCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATc                                                                         <  1:1613297/140‑1 (MQ=255)
                                                                  gCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTcgtc                                                                         <  1:734681/140‑4 (MQ=255)
                                                                    cTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCa                                                                       >  2:328704/1‑140 (MQ=255)
                                                                    cTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCa                                                                       >  2:1366965/1‑140 (MQ=255)
                                                                       ccccACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGTCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACtt                                                                    >  1:747116/1‑140 (MQ=255)
                                                                       ccccACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACtt                                                                    >  1:1045210/1‑140 (MQ=255)
                                                                       ccccACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACtt                                                                    >  1:676424/1‑140 (MQ=255)
                                                                       ccccACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACtt                                                                    >  1:1721355/1‑140 (MQ=255)
                                                                       ccccACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACtt                                                                    >  1:1614010/1‑140 (MQ=255)
                                                                       ccccACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACtt                                                                    >  1:152518/1‑140 (MQ=255)
                                                                          cacaAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACttttt                                                                 <  2:1441791/140‑1 (MQ=255)
                                                                            aaaaaTTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTgg                                                               <  2:1721355/139‑1 (MQ=255)
                                                                                         aCTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAg                                                  >  2:971490/1‑140 (MQ=255)
                                                                                            tCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATCCCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGtct                                               <  1:280641/140‑1 (MQ=255)
                                                                                            tCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATCCCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGtct                                               <  1:398112/140‑1 (MQ=255)
                                                                                                      tGATATCCTCCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTAc                                     >  2:942350/1‑140 (MQ=255)
                                                                                                      tGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTAc                                     >  2:1766457/1‑140 (MQ=255)
                                                                                                      tGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTAc                                     >  1:1564423/1‑140 (MQ=255)
                                                                                                       gATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTAcc                                    >  2:186629/1‑140 (MQ=255)
                                                                                                        atatCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTAccc                                   >  1:494128/1‑139 (MQ=255)
                                                                                                        atatCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTAc                                     >  1:165351/1‑138 (MQ=255)
                                                                                                        atatCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACca                                   >  1:89946/1‑140 (MQ=255)
                                                                                                        atatCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACca                                   <  1:916624/140‑1 (MQ=255)
                                                                                                        atatCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACca                                   >  1:962960/1‑140 (MQ=255)
                                                                                                        atatCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACca                                   >  1:1293716/1‑140 (MQ=255)
                                                                                                        atatCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACca                                   >  2:480175/1‑140 (MQ=255)
                                                                                                        atatCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACca                                   >  1:606056/1‑140 (MQ=255)
                                                                                                          atCCACCAGATTCAATAAGACTTCCATTAGATCCCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACcaca                                 <  1:33519/140‑1 (MQ=255)
                                                                                                           tCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACCacac                                >  1:317283/1‑140 (MQ=255)
                                                                                                            ccaccaGATTCAATAAGCCTTCCATTAGATCCCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACCACAcc                               <  2:858898/140‑1 (MQ=255)
                                                                                                             caccaGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTAc                                     >  1:1451573/1‑133 (MQ=255)
                                                                                                             caccaGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTATCACACCa                              >  1:947617/1‑140 (MQ=255)
                                                                                                             caccaGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACCACACCa                              >  2:699050/1‑140 (MQ=255)
                                                                                                             caccaGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACCACACCa                              >  2:899381/1‑140 (MQ=255)
                                                                                                             caccaGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACCACACCa                              >  2:1732147/1‑140 (MQ=255)
                                                                                                             caccaGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACCACACCa                              >  2:1109984/1‑140 (MQ=255)
                                                                                                              accaGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACCACACCaa                             >  2:749283/1‑140 (MQ=255)
                                                                                                               ccaGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACCACACCAAt                            <  1:631676/140‑1 (MQ=255)
                                                                                                               caaGATTAAATAAGACTTCCATTAGATCCCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACCACACCAAt                            <  2:1586133/138‑1 (MQ=255)
                                                                                                                  gATTCAATAAGACTTCCATTAGATCCCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACCACACCAATAAc                         <  2:1383639/140‑1 (MQ=255)
                                                                                                                     tCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACCACACCAATAACAtt                      >  1:831502/1‑140 (MQ=255)
                                                                                                                      aaaaaaGACTTCCATTAGATCCCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACCACACCAATAACATta                     <  2:152518/136‑1 (MQ=255)
                                                                                                                        aTAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACCACACCAATAACATtagt                   >  1:861591/1‑140 (MQ=255)
                                                                                                                              cTTCCATTAGATCCCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTGCAGTCTCATCGTTTACCACACCAATAACATTAGTAGTGaa             <  1:1277115/140‑1 (MQ=255)
                                                                                                                              cTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACCACACCAATAACATTAGTAGTGaa             <  1:1766457/140‑1 (MQ=255)
                                                                                                                                tCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACCACACCAATAACATTAGTAGTGAAgg           <  1:1107589/140‑1 (MQ=255)
                                                                                                                                 ccATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACCACACCAATAACATTAGTAGTGAAGGc          >  2:663336/1‑140 (MQ=255)
                                                                                                                                 ccATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACCACACCAATAACATTAGTAGTGAAGGc          >  2:403793/1‑140 (MQ=255)
                                                                                                                                        aTACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACCACACCAATAACATTAGTAGTGAAGGCAGCCATc   >  2:29230/1‑140 (MQ=255)
                                                                                                                                         tACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACCACACCAATAACATTAGTAGTGAAGGCAGCCATcc  >  1:1062535/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                          
GTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACATTCTGCTGGCCATGGTTGATAATATGAGTGTTATTTGTTGTACCTCGTTCATCCACTTTTTGGTTGCCATCTACAGTCTCATCGTTTACCACACCAATAACATTAGTAGTGAAGGCAGCCATCC  >  NC_000913/2782371‑2782647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑