Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,429,9840TG23.3% 210.7 / 19.0 86L41V (TTG→GTG) cycAserine/alanine/glycine/cycloserine:H(+)symporter
Reads supporting (aligned to +/- strand):  ref base T (9/57);  new base G (20/0);  total (29/57)
Fisher's exact test for biased strand distribution p-value = 5.47e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 108 total aligned reads displayed.

GGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAAT  >  NC_000913/4429845‑4430119
                                                                                                                                            |                                                                                                                                        
ggTACAGGAAGAAAAAAACATGGTAGATCATGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGgt                                                                                                                                          <  2:1700719/140‑1 (MQ=255)
ggTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGgt                                                                                                                                          <  2:540268/140‑1 (MQ=255)
 gTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGgtt                                                                                                                                         <  2:140774/140‑1 (MQ=255)
  tACAGGAAGAAAAAAACTTGGTAGATCAGGTAAAAGCCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCATTCTCCCAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGG‑AGGGGggtg                                                                                                                                        >  2:1111863/1‑140 (MQ=255)
  tACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGTCATTGGT‑ACGGGgttg                                                                                                                                        >  1:1087788/1‑140 (MQ=255)
  tACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGgttg                                                                                                                                        >  2:323312/1‑140 (MQ=255)
  tACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGgttg                                                                                                                                        >  2:1164261/1‑140 (MQ=255)
  tACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGgttg                                                                                                                                        >  1:1255829/1‑140 (MQ=255)
  tACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGggtg                                                                                                                                        >  1:906387/1‑140 (MQ=255)
  tACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑AAGGGggtg                                                                                                                                        >  1:856451/1‑140 (MQ=255)
      ggAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTa                                                                                                                                    <  2:963892/140‑1 (MQ=255)
      ggAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTa                                                                                                                                    <  2:275751/140‑1 (MQ=255)
      ggAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTa                                                                                                                                    <  1:1164261/140‑1 (MQ=255)
      ggAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTa                                                                                                                                    <  1:1119121/140‑1 (MQ=255)
        aagaaAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑g                                                                                                                                 <  2:1781451/140‑1 (MQ=255)
           aaaaaaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑gggg                                                                                                                              >  2:321217/1‑140 (MQ=255)
           aaaaaaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGGTTTTTAT‑gggg                                                                                                                              >  2:1427081/1‑140 (MQ=255)
           aaaaaaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGGTGTTTAT‑gggg                                                                                                                              >  1:1404112/1‑140 (MQ=255)
            aaaaaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTATGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGt                                                                                                                             <  2:1155861/140‑1 (MQ=255)
            aaaaaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGt                                                                                                                             <  2:1493123/140‑1 (MQ=255)
             aaaaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTc                                                                                                                            <  2:926306/140‑1 (MQ=255)
             aaaaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTc                                                                                                                            <  1:1367960/140‑1 (MQ=255)
                   aTGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCaa                                                                                                                      <  2:866104/140‑1 (MQ=255)
                   aTGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCaa                                                                                                                      <  1:1054164/140‑1 (MQ=255)
                   aTGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCaa                                                                                                                      <  1:633830/140‑1 (MQ=255)
                         gATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGAt                                                                                                                <  2:833312/140‑1 (MQ=255)
                         gATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGAt                                                                                                                <  2:1241097/140‑1 (MQ=255)
                         gATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCACTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGGTGTTTAT‑GGGGTCTGGCAAAACGAt                                                                                                                >  1:991235/1‑140 (MQ=255)
                             aGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGGTGTTTTT‑TGGGTCTGGCAAAACGATTAGc                                                                                                            >  2:388910/1‑140 (MQ=255)
                              ggTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATACAGCTTATTGCCATTGGCGGTGGCATTGGG‑ACGGGGGTGTTTAT‑GGGGGCTGGCAAAACGATTAGcc                                                                                                           >  1:834275/1‑140 (MQ=255)
                               gTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑GCGGGGGTGTTTAT‑GGGGGCTGGCAAAACGATTAACCt                                                                                                          >  1:665583/1‑140 (MQ=255)
                               gTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGGCTGGCAAAACGATTAGCCt                                                                                                          >  1:490808/1‑140 (MQ=255)
                                tAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGTGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTTGT‑ACGGGGGTGTTTTT‑TGGGGCTGGCAAAAAGAATAGCCtt                                                                                                         >  2:608743/1‑140 (MQ=255)
                                 aaaaGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGGTGTTTTT‑TGGGGCTGGCAAAAAGATTAGCCttt                                                                                                        >  2:773618/1‑139 (MQ=255)
                                   aaGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGcc                                                                                                      <  2:934914/140‑1 (MQ=255)
                                         ttGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGGCCTTGGTGACGGGG‑TGTTTATCGGGGCCCGCCAAAAC‑ACTAGCCTTGCCGGGCCg                                                                                                >  2:1221407/1‑140 (MQ=255)
                                         ttGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGGCCATGGT‑AAGGGGGTGTTTTT‑GGGGTCTGGCAAAACGATTAGCCTTTCCGGGGCg                                                                                                >  1:1413640/1‑140 (MQ=255)
                                         ttGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCg                                                                                                <  2:1553321/140‑1 (MQ=255)
                                         ttGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGGCTGGCAAAACGATTAGCCTTGCCGGGCCg                                                                                                >  1:610160/1‑140 (MQ=255)
                                                   tCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAttt                                                                                      <  2:1415845/140‑1 (MQ=255)
                                                    cAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAtttt                                                                                     <  1:773618/140‑1 (MQ=255)
                                                    cAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAtttt                                                                                     <  1:1700018/140‑1 (MQ=255)
                                                     aGGCTCCGGCTGAACAGTCGCTACGGTGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTc                                                                                    <  1:608743/140‑1 (MQ=255)
                                                      ggCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCGTTGGT‑ACGGGGGTGTTTAT‑GGGGGCTGGCAAAACGATTAGCCTTGCCGGGCCGGCGATCAtttttt                                                                                   >  2:1388104/1‑138 (MQ=255)
                                                      ggCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCg                                                                                   <  2:1310518/140‑1 (MQ=255)
                                                        cTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGtt                                                                                 <  1:388910/140‑1 (MQ=255)
                                                           cGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGttt                                                                                >  2:1504018/1‑138 (MQ=255)
                                                           cGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGttt                                                                                <  1:1504018/138‑1 (MQ=255)
                                                           cGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGGTGTTTTT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGGCGTCGGTCATTTTTGttttt                                                                              >  1:709572/1‑138 (MQ=255)
                                                            ggCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTtata                                                                             <  2:1255829/140‑1 (MQ=255)
                                                            ggCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTtata                                                                             <  2:491074/140‑1 (MQ=255)
                                                            ggCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTtata                                                                             <  2:610160/140‑1 (MQ=255)
                                                             gCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCATCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTatat                                                                            <  2:1233892/140‑1 (MQ=255)
                                                             gCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTatat                                                                            <  2:499719/140‑1 (MQ=255)
                                                             gCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTatat                                                                            <  2:1371393/140‑1 (MQ=255)
                                                             gCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTatat                                                                            <  2:331589/140‑1 (MQ=255)
                                                                 aaCAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGGTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATc                                                                        >  2:1000776/1‑140 (MQ=255)
                                                                   cAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCAt                                                                      <  1:744435/140‑1 (MQ=255)
                                                                     gTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTg                                                                    <  2:436305/140‑1 (MQ=255)
                                                                      tCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTTCCATTGGT‑ACGGGGGTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGGTCATTTTCGTTTATATGATCATTgg                                                                   >  1:1125514/1‑140 (MQ=255)
                                                                       cGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGGTGTTTTT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGt                                                                  >  1:22966/1‑140 (MQ=255)
                                                                        gCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGtt                                                                 <  2:490808/140‑1 (MQ=255)
                                                                        gCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGGTGTTTAT‑GGGGGCTGGCAAAAAGATTAGCCTTGCCGGGGCGTCGGTCATTTTCGTTTTTATGATCATTGGtt                                                                 >  2:1071485/1‑140 (MQ=255)
                                                                          tACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGtttt                                                               <  1:1111863/140‑1 (MQ=255)
                                                                          tACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGtttt                                                               <  1:323312/140‑1 (MQ=255)
                                                                               cgcAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGct                                                          <  1:163077/140‑1 (MQ=255)
                                                                                gcAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGT‑ACGGGGGTGTTTAT‑TGGGTCTGGCCAAACGATTAGCCTTGCCGGGGCGGCGATCATTTTCGTTTATATGATCATTTGTTTTATGctc                                                         >  2:688879/1‑140 (MQ=255)
                                                                                 cAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGctct                                                        <  1:346370/140‑1 (MQ=255)
                                                                                  aaTCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTTGT‑ACGGGGGTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCtt                                                       >  1:682493/1‑140 (MQ=255)
                                                                                     ctcACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCttttt                                                    <  2:13502/140‑1 (MQ=255)
                                                                                           aaCCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGAt                                              <  1:1071485/140‑1 (MQ=255)
                                                                                            aCCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATg                                             <  1:321217/140‑1 (MQ=255)
                                                                                              cGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCg                                           <  1:537326/140‑1 (MQ=255)
                                                                                              cGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCg                                           <  2:252208/140‑1 (MQ=255)
                                                                                                aCATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCggg                                         <  1:412749/140‑1 (MQ=255)
                                                                                                aCATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCggg                                         <  2:451824/140‑1 (MQ=255)
                                                                                                aCATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCggg                                         <  1:231662/140‑1 (MQ=255)
                                                                                                 cATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGc                                        <  2:1601283/140‑1 (MQ=255)
                                                                                                   tatTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCaa                                      <  1:1553157/140‑1 (MQ=255)
                                                                                                          cTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGGTGTTTAT‑GGGGTCTGGCAAAAAGATTAGCCTTGCCGGGGCGTCGATCATTTTTGTTTATATGATCATTGGTTTTTTGCTCTTTTTTGTGATGCGGGCAATgggggg                               >  2:1378516/1‑139 (MQ=255)
                                                                                                              ttGCCATTGGCGGTGCCATTGGT‑ACGGGGGTGTTTAT‑GGGGTCTGGCAAAACGATTAACCTTGCCGGGGCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATtg                           >  1:1546148/1‑140 (MQ=255)
                                                                                                               tGCCATTGGCGGTGCCATTGGT‑ACGGGGGTGTTTTT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATtgc                          >  2:1185199/1‑140 (MQ=255)
                                                                                                               tGCCATTGGCGGTGCCATTGGT‑ACGGGGGTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCg                                           >  1:567232/1‑123 (MQ=255)
                                                                                                               tGCCATTGGCGGTGCCATTGGG‑ACGGGGGTGTTTTT‑TTGGGCTGGCAAAACGAATAGCCCTGCCGGGGCGGCGATCATTTTTGTTTTTATGATCATTTGTTTTTTGCTCTTTTTTGTGATGCGGGGAATGGGGGGATtgt                          >  2:385896/1‑139 (MQ=255)
                                                                                                               tGCCATTGGCGGGGCCATTGGT‑GCGGGGTTGTTTTT‑GGGGGCTTGCAAAACAAGTAGACTTGCTCGGCCGTCGATCAATTTTGGTTATATTTTCCTTGGTTTTTTTTTCTTTTTCCTGATGCGGGCAATGGGGGACTtgc                          >  2:1133966/1‑140 (MQ=255)
                                                                                                               tGCCATTGGCGGGGACATTTGT‑ACGGGGGTGTTTTT‑GGGGTCTGGCAAAACGATTAGCCCTTCCGGGGCGTCGCTCATTTTTGGTTTTATTATCATTGGGTTTTTTCTCTTTTTTGTGATGCGGGCGATGGGGGAATtgc                          >  2:1129104/1‑140 (MQ=255)
                                                                                                                  cATTGGCGGTGCCATTGGT‑ACGGGGGTGTTTAT‑GGGGGCTGGCAAAAAGATTAGCCTTGCCGGGGCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGGATtgctgc                       >  2:204413/1‑140 (MQ=255)
                                                                                                                    ttGGCGGTGCCATTGGT‑ACGGGGGTGTTTTT‑GGGGGCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTTTGCTCTTTTTCGTGATGCGGGCAATGGGGGGATTGCTGCtt                     >  2:917367/1‑140 (MQ=255)
                                                                                                                     tGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCttt                    <  2:856451/140‑1 (MQ=255)
                                                                                                                        cGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGa                 <  1:144341/140‑1 (MQ=255)
                                                                                                                        cGGTGCCATTGGT‑ACGGGGGTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGGAATGGGGGAATTGCTGCTTTCGa                 >  1:938016/1‑140 (MQ=255)
                                                                                                                          gTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAAt               <  2:1601955/140‑1 (MQ=255)
                                                                                                                                ttGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTGCGAATCTGGaa         <  1:1221407/140‑1 (MQ=255)
                                                                                                                                 tGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAAt        >  2:1540643/1‑140 (MQ=255)
                                                                                                                                   gT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATAc      <  2:1379701/140‑1 (MQ=255)
                                                                                                                                   gT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATAc      <  1:1000776/140‑1 (MQ=255)
                                                                                                                                      aCGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACaa    <  2:133957/140‑1 (MQ=255)
                                                                                                                                      aCGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACaa    >  1:768712/1‑140 (MQ=255)
                                                                                                                                        ggggTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAAt  <  2:665583/140‑1 (MQ=255)
                                                                                                                                        ggggTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAAt  >  1:223019/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                        
GGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGT‑ACGGGGTTGTTTAT‑GGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAAT  >  NC_000913/4429845‑4430119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑