Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	28.1%	109.1 / 29.4	64	intergenic (‑179/‑28)	ttdR/ttdA	DNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (4/42);  new base T (18/0);  total (22/42)
Fisher's exact test for biased strand distribution p-value = 2.03e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCA‑‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAA  >  NC_000913/3206298‑3206574                                                                                                                                           |                                                                                                                                              aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCa                                                                                                                                             >  1:1949333/1‑140 (MQ=255) aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCa                                                                                                                                             <  2:850422/140‑1 (MQ=255)   aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGa                                                                                                                                           <  1:1912369/140‑1 (MQ=255)   aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGa                                                                                                                                           >  1:1239164/1‑140 (MQ=255)     cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACa                                                                                                                                         <  1:1785007/140‑1 (MQ=255)     cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACa                                                                                                                                         <  2:84422/140‑1 (MQ=255)          ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATAc                                                                                                                                    <  1:1212487/140‑1 (MQ=255)             ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACaaa                                                                                                                                 <  2:121832/140‑1 (MQ=255)               cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCCGACAAATACaaaaa                                                                                                                               >  1:1982984/1‑140 (MQ=255)               cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGAcaaaaacaaaaa                                                                                                                               >  1:1541718/1‑140 (MQ=255)                 cTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCCCATTTTCAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACt                                                                                                                             <  2:2083494/140‑1 (MQ=255)                  tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTg                                                                                                                            <  2:1660421/140‑1 (MQ=255)                  tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTg                                                                                                                            <  2:1090411/140‑1 (MQ=255)                   gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTgg                                                                                                                           <  2:781831/140‑1 (MQ=255)                   gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTgg                                                                                                                           <  2:1949333/140‑1 (MQ=255)                    ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAAAAAAAAAAAAAAAATGGa                                                                                                                          >  2:1379917/1‑140 (MQ=255)                     tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                         <  2:1239164/140‑1 (MQ=255)                     tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                         <  2:2021342/140‑1 (MQ=255)                     tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                         <  1:697812/140‑1 (MQ=255)                         gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTCAAAAAAAAAAAAAAAATTGAGTTgg                                                                                                                     >  2:2003775/1‑139 (MQ=255)                              atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCCGACAAAAACAAAAAAAGGAGTTGCCctga                                                                                                                >  1:2001521/1‑140 (MQ=255)                              atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatga                                                                                                                >  2:1824936/1‑140 (MQ=255)                              atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatga                                                                                                                <  1:647276/140‑1 (MQ=255)                                  tcattcTTATTTTAGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAg                                                                                                            <  1:110513/140‑1 (MQ=255)                                    attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCg                                                                                                          <  1:1835125/140‑1 (MQ=255)                                     ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                         <  1:1379917/140‑1 (MQ=255)                                     ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                         <  2:1496181/140‑1 (MQ=255)                                         tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAACAAAAAATGGAGGTGGCATGATGAggggaaag                                                                                                     >  1:243029/1‑133 (MQ=255)                                         tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAAAAAAAAAAGGGGGTGCCATGATTAGGGaaaaa                                                                                                     >  2:1566851/1‑138 (MQ=255)                                           ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAACAAAAAATGGAGTTGCCATGATGAGCgaaagaaa                                                                                                   >  1:1071995/1‑140 (MQ=255)                                             ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAATACAAAAACTGGAGTTGCCATGATGAGGGAAAAaaata                                                                                                 >  2:1607054/1‑140 (MQ=255)                                              tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTTC‑GACAAAAACAAAAAAAGGGGGTGCGATGATGAGCGAAAGtaaaaa                                                                                                >  2:899532/1‑140 (MQ=255)                                              tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCACACAAAAAAAAAAAATGGAGGTGGCATGATGAGGGGAAGaaaaaa                                                                                                >  2:1412835/1‑140 (MQ=255)                                                gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGc                                                                                              <  1:74027/140‑1 (MQ=255)                                                      aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                        <  1:1412835/140‑1 (MQ=255)                                                       ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                       <  1:2003775/140‑1 (MQ=255)                                                           cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCA‑‑GTg                                                                                 <  2:1577782/140‑1 (MQ=255)                                                                tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCATACAAAAAAAAAAAATGGGGTTGCGATGATGAGCGAAAGAAATAAACAACAAGCA‑‑GAGAATaa                                                                            >  2:587420/1‑140 (MQ=255)                                                                tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCA‑‑GTGAATaa                                                                            <  1:1824936/140‑1 (MQ=255)                                                                tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACA‑TTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAACAAAAAATGGAGTTGCCATGATGAGGGAAAGAAATAAAAAAAAGGCA‑‑GTGAaaaaa                                                                           >  1:1232676/1‑139 (MQ=255)                                                                 gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCA‑‑GTGAATAAg                                                                           <  1:1246858/140‑1 (MQ=255)                                                                    aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCA‑‑GTGAATAAGTTg                                                                        <  2:1331402/140‑1 (MQ=255)                                                                         cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCA‑‑GTGAATAAGTTGACaga                                                                   <  1:899532/140‑1 (MQ=255)                                                                          atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTCCAACAAAAAAAAAAAAAGGAGGTGTCATGATGAGCGAAAAGAAAAAACAACAAGCA‑‑GTGAATAAAATGTCagaa                                                                  >  2:1015905/1‑139 (MQ=255)                                                                          atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTCAGACAAATAAAAAAAATGGAAATGGCATTATGTGGGGAAAAAATAAACAACAAGCA‑‑GTGAATAAATTTATagag                                                                  >  2:129791/1‑140 (MQ=255)                                                                           tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCA‑‑GTGAATAAGTTGACAgaga                                                                 <  2:367294/140‑1 (MQ=255)                                                                            atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGAATTTTTCAGACAAAAACAAAAAATGGAGTTGCCATGATGAGCGGAAATAAAAAGCAAAAGGCA‑‑GGGAAAAAAATGACAGAGAt                                                                >  2:1680055/1‑140 (MQ=255)                                                                            atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCAGACAAATAAAAAAAAAGGAGTTGGCATGATGAGGGAAAAGAAAAAACAAAAAGCA‑‑AGGAAAAAAATTACAGAGAt                                                                >  2:1892678/1‑140 (MQ=255)                                                                                  ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAACAAAAAATGGAGTTGGCATGATGAGCGGAAAAAAAAAACAAAAAGCA‑‑GTGAATAAATTGACAGAGATTGTCGc                                                          >  2:958496/1‑140 (MQ=255)                                                                                     ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCCGACAAAAAAAAAAAATTGGGGTGGCCTGATGAGGGGAAGAAATAAACAAAAAGCA‑‑AGGAATAAAATGACAAAGAATGTTGCTaa                                                       >  2:446585/1‑140 (MQ=255)                                                                                      gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTTAGAAAAAAAAAAAAAATGGAGTTGCCATGGTGGGGGAAAAGAAAAAAAAAAAGGGA‑‑GGGAAAAAAATTACAGAGAATTTCGCTaaa                                                      >  2:39871/1‑139 (MQ=255)                                                                                      gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCCGACAAAAACAAAAAATGGAGGTGGCATGATGAGCGAAAGGAATAAACAAAAAGCA‑‑GTGAAAAAATTGACAAAGATTGTTGCTAAc                                                      >  2:887909/1‑140 (MQ=255)                                                                                        aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGAATTTTTCAAACAAAAAAAAAAAATGGAGTTGGCATGGTGAGGGGAAGTAAAAAACAAAAAGCA‑‑GTGAATAAGATGACAGAGAATTTCGCTAAAtt                                                    >  2:1208107/1‑140 (MQ=255)                                                                                        aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGAATTTTTCAAACAAAAAAAAAAAAAGGAGGTGGCATGATGAGGGGAAAAAAAAAAAAACAAGCA‑‑GTGAAAAAAATGACAGAGATTGTTGCTAACtt                                                    >  2:1905648/1‑140 (MQ=255)                                                                                                    aCCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCA‑‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAt                                        <  1:1680055/140‑1 (MQ=255)                                                                                                        caTTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAACAAAAAATGGGGGTGTCCTGATGTGGGGAAGTAAAAAACAACAGGCA‑‑GGGAATAAATTGACAGAGATTGTCGCTAACTTTTCCCCCCTGATTTCt                                    >  2:1080795/1‑140 (MQ=255)                                                                                                        caTTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAAACAAAAAAAAAAAATGGAGTTTGCATGATGAGCGAAAAGAAAAAACAAAAAGCA‑‑GAGAAAAAATTGAAAGAAAATGTCGCTAACTTTACCGCCATGAttttt                                    >  2:1908965/1‑138 (MQ=255)                                                                                                        caTTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCCGACAAAAAAAAAAAAAGGAGGTGCCATGATGAGGGAAAGGAAAAAACAACAAGCA‑‑GTGAATAAATTGACAGAAAATTTCGCTAAATTTTACCCCCTGAttttt                                    >  2:814397/1‑138 (MQ=255)                                                                                                                gTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCA‑‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAAt                            <  1:1908965/140‑1 (MQ=255)                                                                                                                    tAGTTTGTGGCGGT‑GAATTTTCCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCA‑‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCt                        <  1:1379632/140‑1 (MQ=255)                                                                                                                       tttGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCA‑‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgat                     <  1:1080795/140‑1 (MQ=255)                                                                                                                       tttGTGGCGGT‑GAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCA‑‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgat                     <  1:1208107/140‑1 (MQ=255)                                                                                                                        ttGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGATTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCA‑‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgatg                    <  2:1071995/140‑1 (MQ=255)                                                                                                                        ttGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCA‑‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgatg                    <  1:587420/140‑1 (MQ=255)                                                                                                                        ttGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCA‑‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgatg                    <  1:1607054/140‑1 (MQ=255)                                                                                                                           tgGCGGT‑GAAATTTTTCCGACAAAAACAAAAAATTGAGTTGCCATGATGAGCGAAAAAAAAAAACAAAAAGCA‑‑GTGAATAAATTGACAGAGAATTTTGCTAACTTTACCCCCATGATTTTTACCCGAAAGCCTGATGACg                 >  2:1311062/1‑140 (MQ=255)                                                                                                                             gCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCA‑‑GTGAATAAGTTGACAGAGATTGTCGCT‑ACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtgg              <  2:1982984/140‑1 (MQ=255)                                                                                                                               ggT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg               <  1:1788591/140‑1 (MQ=255)                                                                                                                                   gAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCA‑‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa          <  1:1015905/140‑1 (MQ=255)                                                                                                                                   gAATTTTTCAAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCA‑‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa          <  1:887909/140‑1 (MQ=255)                                                                                                                                   aaaTTTTTCCAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCA‑‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa          <  1:958496/139‑1 (MQ=255)                                                                                                                                   aaaTTTTTCCAAAAAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCA‑‑GTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa          <  2:1232676/139‑1 (MQ=255)                                                                                                                                         ttCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCA‑‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACt    >  2:1092103/1‑140 (MQ=255)                                                                                                                                           ccAGAAAAAAACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCACCAGGCA‑‑GTGAATAAGTTGACAGAGATTGTCGTAAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa  <  2:212330/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                              AAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCA‑‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAA  >  NC_000913/3206298‑3206574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑