Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,628,625	0	A	C	23.4%	142.4 / 10.3	64	V105G (GTC→GGC)	pdeF	cyclic di‑GMP phosphodiesterase PdeF
Reads supporting (aligned to +/- strand):  ref base A (45/4);  new base C (0/15);  total (45/19)
Fisher's exact test for biased strand distribution p-value = 2.43e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCT  >  NC_000913/2628486‑2628758                                                                                                                                             |                                                                                                                                      gCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGa                                                                                                                                       <  2:1691928/140‑1 (MQ=255)  cTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGAc                                                                                                                                      >  2:528238/1‑140 (MQ=255)  cTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGAc                                                                                                                                      <  2:2235998/140‑1 (MQ=255)  cTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGAc                                                                                                                                      >  1:702693/1‑140 (MQ=255)    cGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGAccc                                                                                                                                    <  1:569336/140‑1 (MQ=255)       cAGTAATCCTAAAAATGCAGAAAACTGAAAAAGTATCAAAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATCCGCGGGGAAATA‑AAACGGGTATCCCCATGTG‑AACGTTTTTACGCGGGGGAGTAAAGCCCCTGTa                                                                                                                                <  2:98656/140‑1 (MQ=255)          tAATCCAAAAAATGCAGCAAACGGGAAAAGTATCAAAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATCCGCGGGGAAATT‑AACCGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGAAAAGCCCCTGTAAc                                                                                                                              <  2:190794/140‑1 (MQ=255)          aaaTCCTACAAATGCAGCAAACGGAAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCAAGAATATACGCTGGAAAATA‑AAACGGGTATCACCATGTAAAACGTTGTTCCGCGGGGGAGAAAAGCCCCTGTAAc                                                                                                                              <  2:1330452/139‑1 (MQ=255)               caaCAAATGCAAAAAACTGAAAAAATATCAAAAACAGCGTTGCAGGAAACACAATCTCCCAGAATAGCCGCGGGGAAATT‑AAAGGGTTACCACCATGTGAAACTTTTTTACGCGGGGGAGAAAAGCCCCTGAAACCGccc                                                                                                                         <  2:1329293/138‑1 (MQ=255)               cTAAAAATGCAGAAAACTGAAAAAGTATAAGAAACAGCGTTGAAGAAAACACAATCGGCCAGAATATACGCGGGAAAATA‑AACCGGGTATCACCATGTAAAACGTTGTTACGCCGGGGAGAAAAGCCCCTGAAACCGccc                                                                                                                         <  2:1755592/140‑1 (MQ=255)                  aaaaTGCAGCAACCGGGAAAAATATCAAAAACAGCTTTGGAGGAAACACAATACGCGAGAATATGCCCGGATAAATT‑AAACGGTTATCCCCATGTGAAACTTTTTTACGGGGGGGAGAAAAGACCCTGTAACCGCCCCaa                                                                                                                      <  2:1855199/139‑1 (MQ=255)                   aaaTGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAAc                                                                                                                     >  1:1769675/1‑140 (MQ=255)                      ggcagaaAACTGAAAAAGTATCAAAAACAGCGTTGCAGAAAACACAATCTGCCAGAATATCCGCTGGAAAATT‑AAACGGGTACCCCCATGTGAAACGTTGTTCCGCCGGGGAGTAAAGCCCCTGTAACCGCCCCAACaga                                                                                                                  <  2:983503/139‑1 (MQ=255)                       gcagaaAACGGAAAAAGTATCAAAAACAGCGTTGCAGAAAACACAATCTGCAAAAATATCCGCTGGAAAATA‑AAACGGGTATCACCATGTAAAACGTTGTTCCGCCGGGGAGAAAAGCCCCTGTAACCGCCCCAACagag                                                                                                                 <  2:1910008/140‑1 (MQ=255)                        cagcaAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAgaga                                                                                                                >  2:1879079/1‑140 (MQ=255)                                              aaaCGGCGTTGCAGAAAACAAATTCGGACAAAATATCCGC‑GGAAAATTAAAACGGGTATCACAATGTAAACCGTTTTTCCGGGGGGAAGAAAACCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAg                                                                                          <  2:2038287/140‑1 (MQ=255)                                              aaaCAGCTTTGAAGGAAAAAAAATCCGCCAAAAAAAACGGGGGAAAATA‑AAACGGGTATCACCATGTGAAACGTTGTTACGCGGGGGAGAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAg                                                                                          <  2:1409139/140‑1 (MQ=255)                                              aaaCAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAg                                                                                          <  1:69775/140‑1 (MQ=255)                                              aaaCAGCGTTGCAGAAAACACAATCTGCCAAAATATACGCGGGAAAATA‑AAACGGGTACCACCATGTAAAACTTTGTTACGCGGGGGAGAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAg                                                                                          <  2:2012973/140‑1 (MQ=255)                                              aaaCAGCGTTGCAGAAAACAAAATCTGCAAAAATATACGCTGGGAAATA‑AAACGGGTACCACCATGTAAAACGTTGTTACGCGGGGGAGAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAg                                                                                          <  2:1918249/140‑1 (MQ=255)                                              aaaCAGCGTGGCAGAAAACACAATCTCCCAAAATATCCGCGGGAAAATA‑AACCGGTTATCCCCATGTAAAATGTTGTTCCGCCGGGGAGAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAg                                                                                          <  2:1946560/140‑1 (MQ=255)                                                 cAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATA‑AAAGGGGTATCACCATGTAAACGGTTGTTACGCC‑GGGAGTAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGt                                                                                      <  2:442979/140‑1 (MQ=255)                                                   gCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGT‑ACCGCCCCAACAGGGAATGATAATGATAATAAAATTAACCGTTa                                                                                    >  2:1769888/1‑140 (MQ=255)                                                    cGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTa                                                                                    >  1:2165253/1‑140 (MQ=255)                                                        gCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGa                                                                                >  1:1366508/1‑140 (MQ=255)                                                        gCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGa                                                                                >  1:997440/1‑140 (MQ=255)                                                            gAAACACAATCTGCCAGAATATACGCTGGGAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGc                                                                            >  2:726082/1‑140 (MQ=255)                                                              aaCACAATCTGCCAGAATATACGCTGGGAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGt                                                                          >  1:1662516/1‑140 (MQ=255)                                                                 acaATCTGCCAGAATATACGCTGGGAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTc                                                                       >  1:1699321/1‑140 (MQ=255)                                                                  caATCTGCCAGAATATACGCTGGGAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCg                                                                      >  1:2177097/1‑140 (MQ=255)                                                                     tCTGCAAAAATATCCGCGGGAAAATA‑AACCGGGTATCCCCATGTGAAACGTTGTTACGCCGGGGAGAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATTATAATAAAATTAGCCGTTAATGATAGCGTTTCGTaa                                                                   <  2:82197/140‑1 (MQ=255)                                                                       tGCCAGAATATACGCTGGGAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTaaaa                                                                 >  1:2208880/1‑140 (MQ=255)                                                                             aaTACACGCTGGGAAATA‑AAGCGGGTACCACCATGTAAAACGTTTTTCCGCCGGGGAGTAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAg                                                           <  1:1879079/140‑1 (MQ=255)                                                                               tatcCGCGGGAAAATA‑AAACGGGTACCCCCATGTAAAACGTTGTTACGCGGGGGAGAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGct                                                         <  2:1769675/140‑1 (MQ=255)                                                                               tataCGCGGGAAAATA‑AAACGGGTATCCCCATGTAAAAGTTTTTTCCGCGGGGGAGAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGct                                                         <  2:2025997/140‑1 (MQ=255)                                                                                  aCGCTGGGAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTg                                                      >  1:2025997/1‑140 (MQ=255)                                                                                   cGCTGGGAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGc                                                     >  1:1435262/1‑140 (MQ=255)                                                                                       gggAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCatat                                                 >  1:1165263/1‑140 (MQ=255)                                                                                       gggAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCatat                                                 >  2:784925/1‑140 (MQ=255)                                                                                       gggAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCatat                                                 >  2:2138544/1‑140 (MQ=255)                                                                                       gggAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCatat                                                 >  1:1960131/1‑140 (MQ=255)                                                                                       gggAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCatat                                                 >  2:69021/1‑140 (MQ=255)                                                                                        ggAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATAtt                                                >  1:2085987/1‑140 (MQ=255)                                                                                        ggAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATAtt                                                >  1:128770/1‑140 (MQ=255)                                                                                        ggAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATAtt                                                >  2:156230/1‑140 (MQ=255)                                                                                        ggAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTACGTAAAAACCCAGCTCTGCATAtt                                                >  2:1888769/1‑140 (MQ=255)                                                                                         gAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATAttt                                               >  1:2202222/1‑140 (MQ=255)                                                                                         gAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATAttt                                               >  2:2104406/1‑140 (MQ=255)                                                                                           aaTT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGc                                             >  2:1265157/1‑140 (MQ=255)                                                                                                 aaCGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACa                                        >  2:2134692/1‑140 (MQ=255)                                                                                                   ggggTATCACCATGTAAAACTTTGTTACGCCGGGGAGAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACaaa                                      <  2:1960131/139‑1 (MQ=255)                                                                                                   cGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACaaa                                      <  1:726082/140‑1 (MQ=255)                                                                                                   cGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACaaa                                      >  2:1054441/1‑140 (MQ=255)                                                                                                        aTCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATcc                                 >  1:87562/1‑140 (MQ=255)                                                                                                          ccccttgtaAAAGGTTGTTACGCGGGGGAAAAAAGCCCCTATAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCt                               <  2:2177097/135‑1 (MQ=255)                                                                                                             cATGTGAAGCGTTGTTACGCCGGGGAGTAAAGCCCCTG‑AACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAgg                           <  1:1769888/140‑1 (MQ=255)                                                                                                              aTGTAAAACGTTTTTCCGCCGGGGAGAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAgg                           <  2:702693/140‑1 (MQ=255)                                                                                                               tgtgAAACTTTGTTACGCGGGGGAGTAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGc                          <  2:2208880/140‑1 (MQ=255)                                                                                                               tgtgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCTGACAAAAATCCCTAAGGc                          >  2:2193893/1‑140 (MQ=255)                                                                                                               tgtgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGc                          >  2:1685534/1‑140 (MQ=255)                                                                                                                gtgAAACGTTGTTACGCCGGGGAGTAAGGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCg                         >  1:758269/1‑140 (MQ=255)                                                                                                                gtgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCg                         >  1:1523382/1‑140 (MQ=255)                                                                                                                gtgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCg                         >  2:718744/1‑140 (MQ=255)                                                                                                                gtgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCg                         >  2:1409384/1‑140 (MQ=255)                                                                                                                gtgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCg                         >  2:297479/1‑140 (MQ=255)                                                                                                                gtgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCg                         >  1:1469673/1‑140 (MQ=255)                                                                                                                gtgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGAC‑AAAATCCCTAAGGCGa                        >  1:1298487/1‑140 (MQ=255)                                                                                                                 tgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTGACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGa                        >  1:1340924/1‑140 (MQ=255)                                                                                                                 tgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGa                        >  1:1877743/1‑140 (MQ=255)                                                                                                                     acgctgttACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATccc                    >  2:747536/5‑140 (MQ=255)                                                                                                                       gttgttACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTATTGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTg                  >  2:1120884/1‑140 (MQ=255)                                                                                                                       gttgttACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTg                  >  2:1598905/1‑140 (MQ=255)                                                                                                                        ttgtgACGCGGGGGAGAAAGGCCCTTGAAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTgg                 <  1:528238/140‑1 (MQ=255)                                                                                                                                ccGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATgccg         <  1:1265157/140‑1 (MQ=255)                                                                                                                                 gggggAAAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATgccgc        <  2:87562/139‑1 (MQ=255)                                                                                                                                   gggAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGccc      <  2:1699321/140‑1 (MQ=255)                                                                                                                                   gggAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGccc      >  2:1444115/1‑140 (MQ=255)                                                                                                                                     gagaAAGGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCTGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAg    <  1:2193893/140‑1 (MQ=255)                                                                                                                                     gagaAAAGCCCCTGAAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCACCCAg    <  1:1764935/140‑1 (MQ=255)                                                                                                                                       gaaaaGCCCTTTTAGCCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCt  <  2:1469673/138‑1 (MQ=255)                                                                                                                                             |                                                                                                                                      GCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATT‑AAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCT  >  NC_000913/2628486‑2628758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑