Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,556,205	0	A	C	22.6%	117.3 / 10.0	53	S215R (AGT→AGG)	rtcA	RNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base A (38/3);  new base C (0/12);  total (38/15)
Fisher's exact test for biased strand distribution p-value = 1.71e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGC‑GAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCG  >  NC_000913/3556066‑3556344                                                                                                                                              |                                                                                                                                            ccaccTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGC‑GAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGaaaaa                                                                                                                                             >  2:1870370/1‑140 (MQ=255)   accTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGC‑GAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACt                                                                                                                                           >  2:275682/1‑140 (MQ=255)        ggCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGC‑GAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCg                                                                                                                                      >  1:1538187/1‑140 (MQ=255)            cTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGC‑GAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAg                                                                                                                                  >  2:931913/1‑140 (MQ=255)               aCGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGC‑GAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAgtgt                                                                                                                               >  1:456389/1‑140 (MQ=255)               aCGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGC‑GAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAgtgt                                                                                                                               >  2:2065958/1‑140 (MQ=255)                         ccccGACAAAAAAAAGGCGTTGGGTGATTTTTTCTCTTTCGACTTCAAGC‑GAGGCGGTATTCCCCGGCCCC‑TGGCCGCGCGGCAGTTTATGAATATTCTGTTCATGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCa                                                                                                                     <  2:1882183/138‑1 (MQ=255)                         ccccGACAAAAAAAAAGCTTTCGGTGTTTTTTTCACTTTCGACTTCAAGC‑GAGACGGTATTCCCCGGCCCC‑TGGTCGCGCGGCAGTTTATGAATTTTTTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCa                                                                                                                     <  2:628279/138‑1 (MQ=255)                               cgacAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGC‑GAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCa                                                                                                               >  2:1929071/1‑140 (MQ=255)                                       aaccGTCCGGTGATTTTTTCCTTTTCCATTCAAAGC‑GAGACGTTATTCCCCGGCCCG‑GGGGCGCGCGGAAGTTTATAAATATTCTGTTCATGGAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATg                                                                                                       <  2:727540/137‑1 (MQ=255)                                        aGCTTTCGGTAATTTTTTCATTTCCGCCTCCAAGC‑GAGCCGTTTTTCCCCGCCCCC‑GGGTCGGGGGGCGGTTTAAAAATTTTCTGTTCATGCGGGAAAAACCCCCCCGCCAGTGTAGCTATTTCACGCTCAGCAACATgg                                                                                                      <  2:2031820/140‑1 (MQ=255)                                            ttgggTTATTTTTTCACTTTCGACTTAAAGC‑GAGACGTTATCCCCCGGCCCC‑GGGTCGCGCGGCAGTTTATAAATTTTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGcgcg                                                                                                  <  2:1068563/137‑1 (MQ=255)                                            ttCGGTGATATTTTCACTTTCGACTTCAAGC‑GAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGcgcg                                                                                                  >  1:151449/1‑140 (MQ=255)                                             tgggTGTTTTTTCCTCTTTCGATTTAAAGC‑GAGACGTTATTCCCCGCCCCC‑GGGTCGCGCGGCGGGTTATGAATTTTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCgg                                                                                                 <  2:1631031/138‑1 (MQ=255)                                             tCGGTGATATTTTCACTTTCGACTTCAAGC‑GAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCgg                                                                                                 >  1:1307037/1‑140 (MQ=255)                                                 taatatTTTCTTTTGCGACTT‑AAGCAGAGCCGGTATCACCGGCCCCC‑TGGGCGCGCGCCAGGTTATGAATATTCTCTTCAAGGAGAAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGcaca                                                                                             <  2:1172127/138‑1 (MQ=255)                                                 tGATTTTTCCTCTTTCGACTTCAGGA‑AAGATGGT‑TCACCCCGCCCCGGGGCCGCGCGGCAGTTAATTAATTTTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGcaca                                                                                             <  2:1587971/140‑1 (MQ=255)                                                      ttttCACTTTCGACTTCAAGC‑GAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGc                                                                                        >  1:2213817/1‑140 (MQ=255)                                                           aCTTTCGACTTCAAGC‑GAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATa                                                                                   >  1:1494483/1‑140 (MQ=255)                                                           aCTTTCGACTTCAAGC‑GAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATa                                                                                   >  1:2199028/1‑140 (MQ=255)                                                           aCTTTCGACTTCAAGC‑GAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATa                                                                                   >  1:394712/1‑140 (MQ=255)                                                              ttCGACTTCAGGC‑GAGACGTTATCCCCCGGCCCC‑GGGCCGCGCGGCGGTTTATGAATTTTCTGTTCATGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGaa                                                                                <  2:104647/140‑1 (MQ=255)                                                               tCGACTTCAAGC‑GAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAAc                                                                               >  1:317223/1‑140 (MQ=255)                                                                  aCTTCAAGC‑GAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtc                                                                            >  1:1041169/1‑140 (MQ=255)                                                                         gC‑GAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGc                                                                     >  1:443716/1‑140 (MQ=255)                                                                               aCGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTg                                                                >  2:1855859/1‑140 (MQ=255)                                                                                  ttATTCCCCGCCCCG‑TGGCCCGGGGGCGGG‑TATAAATTTTCTTTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGcaca                                                            <  2:2178583/139‑1 (MQ=255)                                                                                     ttACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAAt                                                          >  1:55107/1‑140 (MQ=255)                                                                                        cccGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGtt                                                       >  2:2232322/1‑140 (MQ=255)                                                                                           ggCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTccc                                                    >  1:2232532/1‑140 (MQ=255)                                                                                                 cgggTCGGGCGGCAGTTTATGAATTTTCTGTTCATGGAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCt                                               <  2:1494483/138‑1 (MQ=255)                                                                                                   ggccgcgggGGAGTTTATAAATTTTCTTTTCAGGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCg                                             <  2:1307037/137‑1 (MQ=255)                                                                                                    gTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGc                                            >  1:996711/1‑140 (MQ=255)                                                                                                     tCGCGCGGAAGTTTATAAATATTCTGTACATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGcc                                           <  2:443716/140‑1 (MQ=255)                                                                                                     ccgcgcgGGAGTTTATAAATATTTTGTTCATGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGcc                                           <  2:2199028/139‑1 (MQ=255)                                                                                                        cgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                        >  1:1252427/1‑140 (MQ=255)                                                                                                        cgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                        >  2:327512/1‑140 (MQ=255)                                                                                                        cgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                        >  2:333846/1‑140 (MQ=255)                                                                                                        cgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                        >  2:679317/1‑140 (MQ=255)                                                                                                        cgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                        >  1:745198/1‑140 (MQ=255)                                                                                                             gggtttATGAATTTTCTGTCCAGGCAGGGAAAACCACCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                   <  1:1870370/136‑1 (MQ=255)                                                                                                             cgggTTATGAATTTTCTTTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                   <  2:1041169/138‑1 (MQ=255)                                                                                                             cAGGTTATGAATATTCTGTTCATGGAGGGAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                   <  2:394712/140‑1 (MQ=255)                                                                                                             cAGGTAATAAATATTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                   <  1:275682/140‑1 (MQ=255)                                                                                                              agtttATGAATATTCTGTTCATGGAGGGAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCaa                                  <  1:1029263/137‑1 (MQ=255)                                                                                                               ggTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAg                                 <  1:1929071/140‑1 (MQ=255)                                                                                                                  tATGAATTTTCTGTTCATGGAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGgtg                              <  1:1968740/140‑1 (MQ=255)                                                                                                                  tATGAATATTCTGTTCATGCGGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGgtg                              <  2:819109/140‑1 (MQ=255)                                                                                                                  tATGAATATTCTGTTCATGCAGGAAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGgtg                              <  1:931913/140‑1 (MQ=255)                                                                                                                         atTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACg                       >  2:1106973/1‑140 (MQ=255)                                                                                                                          ttCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                      >  1:676304/1‑140 (MQ=255)                                                                                                                          ttCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                      >  1:1889030/1‑140 (MQ=255)                                                                                                                           tCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt                     >  1:1770856/1‑140 (MQ=255)                                                                                                                           tCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt                     >  1:2002276/1‑140 (MQ=255)                                                                                                                           tCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt                     >  2:1227665/1‑140 (MQ=255)                                                                                                                             tGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGc                   >  2:1903396/1‑140 (MQ=255)                                                                                                                             tGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGc                   >  2:1319672/1‑140 (MQ=255)                                                                                                                               ttCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                 >  1:353822/1‑140 (MQ=255)                                                                                                                               ttCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                 >  1:1110311/1‑140 (MQ=255)                                                                                                                                    gCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGc            <  1:327512/140‑1 (MQ=255)                                                                                                                                       gggAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCgag         >  2:365676/1‑140 (MQ=255)                                                                                                                                         gAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGAc       >  2:1552842/1‑140 (MQ=255)                                                                                                                                         gAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGAc       >  2:922875/1‑140 (MQ=255)                                                                                                                                             aaCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTcc   <  1:2232322/140‑1 (MQ=255)                                                                                                                                              aCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCg  >  1:435006/1‑140 (MQ=255)                                                                                                                                              |                                                                                                                                            CCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGC‑GAGACGGTATTACCCGGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCG  >  NC_000913/3556066‑3556344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑