Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	506,061	C→G	T383S (ACC→AGC)	ushA →	5'‑nucleotidase/UDP‑sugar hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	506,061	0	C	G	100.0%	207.4 / NA	58	T383S (ACC→AGC)	ushA	5'‑nucleotidase/UDP‑sugar hydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (30/28);  total (30/28)

GCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGACCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAA  >  NC_000913/505931‑506188                                                                                                                                   |                                                                                                                                gCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATggg                                                                                                                        >  2:808131/1‑140 (MQ=255) gCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATggg                                                                                                                        >  2:791205/1‑140 (MQ=255) gCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATggg                                                                                                                        <  2:425787/140‑1 (MQ=255)     aaaaCCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCgg                                                                                                                    <  1:591904/140‑1 (MQ=255)               aaTGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTgg                                                                                                          >  2:579639/1‑140 (MQ=255)               aaTGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTgg                                                                                                          <  2:55307/140‑1 (MQ=255)               aaTGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTgg                                                                                                          <  2:1103364/140‑1 (MQ=255)                aTGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGc                                                                                                         <  2:973589/140‑1 (MQ=255)                aTGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGc                                                                                                         <  2:42955/140‑1 (MQ=255)                aTGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGc                                                                                                         <  2:415639/140‑1 (MQ=255)                  gATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAg                                                                                                       <  1:539162/140‑1 (MQ=255)                            ttATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGa                                                                                             >  1:880248/1‑140 (MQ=255)                             tATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGAt                                                                                            >  2:901250/1‑140 (MQ=255)                                 aCCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCa                                                                                        <  2:1311544/140‑1 (MQ=255)                                  ccGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCAc                                                                                       <  1:173294/140‑1 (MQ=255)                                   cGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACt                                                                                      >  2:1012634/1‑140 (MQ=255)                                      tCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTggg                                                                                   >  2:450557/1‑139 (MQ=255)                                      tCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGt                                                                                   >  1:779333/1‑140 (MQ=255)                                      tCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGt                                                                                   >  1:254014/1‑140 (MQ=255)                                      tCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGt                                                                                   >  2:324690/1‑140 (MQ=255)                                       ccAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTg                                                                                  <  1:791205/140‑1 (MQ=255)                                        cAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCAAAATGGATCGCACTGGTGc                                                                                 >  1:481354/1‑140 (MQ=255)                                         aGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGcc                                                                                <  2:779333/140‑1 (MQ=255)                                                aGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTg                                                                         >  1:744331/1‑140 (MQ=255)                                                aGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTg                                                                         >  2:787626/1‑140 (MQ=255)                                                aGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTg                                                                         >  2:1212866/1‑140 (MQ=255)                                                     aaGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGtg                                                                    >  2:147553/1‑140 (MQ=255)                                                           aGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGc                                                              >  2:51701/1‑140 (MQ=255)                                                            gCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCg                                                             >  1:950111/1‑140 (MQ=255)                                                                  aGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCg                                                       <  1:579639/140‑1 (MQ=255)                                                                        aaTAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTc                                                 <  1:787626/140‑1 (MQ=255)                                                                             gCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGTTCGCACTGGTGCCGACTTTGCGGTGATGAGCGGGGGCGGAATTCGTGAt                                            >  2:1025673/1‑140 (MQ=255)                                                                             gCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGAt                                            <  2:623334/140‑1 (MQ=255)                                                                              cGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGAtt                                           >  2:362094/1‑140 (MQ=255)                                                                                  aCCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTAt                                       <  1:500297/140‑1 (MQ=255)                                                                                  aCCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTAt                                       <  1:1351255/140‑1 (MQ=255)                                                                                  aCCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTAt                                       <  2:744331/140‑1 (MQ=255)                                                                                       tGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAg                                  <  2:254014/140‑1 (MQ=255)                                                                                       tGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAg                                  <  2:690580/140‑1 (MQ=255)                                                                                       tGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAg                                  <  2:512782/140‑1 (MQ=255)                                                                                          tcgtcTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAGCAg                               <  1:1012634/140‑1 (MQ=255)                                                                                           cgtcTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAGCAgg                              >  1:613492/1‑140 (MQ=255)                                                                                             tcTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCg                            >  1:1307824/1‑140 (MQ=255)                                                                                              cTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGa                           >  1:766261/1‑140 (MQ=255)                                                                                                ggAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGata                         <  1:901250/140‑1 (MQ=255)                                                                                                 gAAGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGatat                        <  1:324690/140‑1 (MQ=255)                                                                                                  aaGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATc                       <  2:608954/140‑1 (MQ=255)                                                                                                  aaGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATc                       <  1:854394/140‑1 (MQ=255)                                                                                                   aGGCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCa                      <  2:766261/140‑1 (MQ=255)                                                                                                    ggCGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAg                     <  1:141948/140‑1 (MQ=255)                                                                                                      cGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCGGACTTTGCGGTGATGAGCGGAGGCGTGATTCGTGATTCTATCGAAGCAGGCGATATCAGCt                   >  2:1341562/1‑140 (MQ=255)                                                                                                      cGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCt                   >  1:975585/1‑140 (MQ=255)                                                                                                      cGATCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCt                   >  2:1227760/1‑140 (MQ=255)                                                                                                        aTCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCtat                 >  1:853081/1‑140 (MQ=255)                                                                                                        aTCGTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCtat                 >  1:610989/1‑140 (MQ=255)                                                                                                           gTGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCTATaaa              >  1:968979/1‑140 (MQ=255)                                                                                                            tGACAAAGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCTATaaaa             >  2:761424/1‑140 (MQ=255)                                                                                                                  aGTGCGTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGc       >  1:458485/1‑140 (MQ=255)                                                                                                                       gTTTTGTACAGAGCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGaaa  >  2:569492/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                GCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGGTCGTCTGGAAGGCGATCGTGACAAAGTGCGTTTTGTACAGACCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAA  >  NC_000913/505931‑506188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑