Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	960,217	T→C	intergenic (+122/‑47)	aroA → / → ycaL	3‑phosphoshikimate 1‑carboxyvinyltransferase/periplasmic protease YcaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	960,217	0	T	C	100.0%	264.2 / NA	71	intergenic (+122/‑47)	aroA/ycaL	3‑phosphoshikimate 1‑carboxyvinyltransferase/periplasmic protease YcaL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/53);  total (18/53)

AGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGA  >  NC_000913/960081‑960343                                                                                                                                         |                                                                                                                               aGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAgctgt                                                                                                                             <  2:1303416/140‑1 (MQ=255) aGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAgctgt                                                                                                                             <  2:55665/140‑1 (MQ=255)   ccAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGctgttg                                                                                                                           >  2:277099/1‑140 (MQ=255)   ccAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGctgttg                                                                                                                           <  1:569891/140‑1 (MQ=255)      ggCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATa                                                                                                                        <  1:1364204/140‑1 (MQ=255)         aGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACat                                                                                                                     <  1:1469657/140‑1 (MQ=255)          gCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACata                                                                                                                    <  1:589646/140‑1 (MQ=255)          gCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACata                                                                                                                    <  1:555380/140‑1 (MQ=255)          gCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACata                                                                                                                    <  2:233926/140‑1 (MQ=255)          gCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACata                                                                                                                    <  2:583196/140‑1 (MQ=255)          gCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACata                                                                                                                    <  1:958382/140‑1 (MQ=255)          gCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACata                                                                                                                    <  1:224623/140‑1 (MQ=255)          gCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACata                                                                                                                    <  2:296576/140‑1 (MQ=255)          gCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGCGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACata                                                                                                                    <  1:1441956/140‑1 (MQ=255)           ccTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACataa                                                                                                                   >  2:1283125/1‑140 (MQ=255)            cTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCAGATTGTCGGCGTTTTTTTTCTGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACataat                                                                                                                  <  1:1294418/140‑1 (MQ=255)             tgaatgaaCAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACataata                                                                                                                 <  1:496230/140‑1 (MQ=255)             tgaatgaaCAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACataata                                                                                                                 <  2:789898/140‑1 (MQ=255)              gaatgaaCAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAatat                                                                                                                <  1:1383164/140‑1 (MQ=255)                   aacaacGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTtata                                                                                                           <  2:1381590/140‑1 (MQ=255)                   aacaacGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTtata                                                                                                           <  2:668581/140‑1 (MQ=255)                   aacaacGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATAGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTtata                                                                                                           <  2:1086913/140‑1 (MQ=255)                     caacGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTtatata                                                                                                         >  1:1370848/1‑140 (MQ=255)                     caacGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTtatata                                                                                                         >  2:196878/1‑140 (MQ=255)                     caacGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTtatata                                                                                                         >  2:893617/1‑140 (MQ=255)                     caacGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTtatata                                                                                                         >  2:1551890/1‑140 (MQ=255)                         gggCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGAt                                                                                                     <  1:1536537/140‑1 (MQ=255)                         gggCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGAt                                                                                                     >  1:1216165/1‑140 (MQ=255)                         gggCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTCTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGAt                                                                                                     <  2:589081/140‑1 (MQ=255)                             aataaataGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAAt                                                                                                 <  2:917669/140‑1 (MQ=255)                              ataaataGCCAAATCTTTTTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGTTACATAATATTTATATATGATTAATc                                                                                                >  2:1552583/1‑140 (MQ=255)                               taaataGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCa                                                                                               <  2:1535835/140‑1 (MQ=255)                                    aGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGga                                                                                          >  1:302450/1‑140 (MQ=255)                                        aaaTCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGgatgat                                                                                      <  1:55495/140‑1 (MQ=255)                                         aaTCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATTAACGGATGAtt                                                                                     <  2:1281582/140‑1 (MQ=255)                                         aaTCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGAtt                                                                                     <  2:1217623/140‑1 (MQ=255)                                            ctttctttATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTcac                                                                                  <  1:277099/140‑1 (MQ=255)                                            ctttctttATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTcac                                                                                  <  2:1214691/140‑1 (MQ=255)                                             tttctttATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTcaca                                                                                 <  1:196878/140‑1 (MQ=255)                                               tctttATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATg                                                                               <  1:1283125/140‑1 (MQ=255)                                                 tttATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATgaa                                                                             <  1:1438852/140‑1 (MQ=255)                                                  ttATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATgaag                                                                            <  1:50114/140‑1 (MQ=255)                                                    aTCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATgaagaa                                                                          >  1:1525424/1‑140 (MQ=255)                                                         aaCGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTa                                                                     >  2:921769/1‑140 (MQ=255)                                                         aaCGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTa                                                                     >  2:1118200/1‑140 (MQ=255)                                                            gTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAAt                                                                  <  1:534214/140‑1 (MQ=255)                                                               ggCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGCAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTAc                                                               >  1:479910/1‑140 (MQ=255)                                                                      tGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCTCATGAAGAATACTAAATTActg                                                             <  1:726329/135‑1 (MQ=255)                                                                      tGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTActg                                                             >  2:726329/1‑135 (MQ=255)                                                                      tGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGc                                                        >  1:267598/1‑140 (MQ=255)                                                                              ttttttttCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGa                                                >  1:1204436/1‑140 (MQ=255)                                                                               tttttttCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGAc                                               <  2:483016/140‑1 (MQ=255)                                                                               tttttttCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGAc                                               <  1:1095945/140‑1 (MQ=255)                                                                               tttttttCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGAc                                               <  2:1370848/140‑1 (MQ=255)                                                                               tttttttCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGAc                                               <  1:1551890/140‑1 (MQ=255)                                                                                    ttCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTg                                          <  2:579839/140‑1 (MQ=255)                                                                                      cGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCCATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTgca                                        <  1:1064768/140‑1 (MQ=255)                                                                                      cGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTgca                                        <  2:1216165/140‑1 (MQ=255)                                                                                      cGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTgca                                        <  2:302450/140‑1 (MQ=255)                                                                                      cGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTgca                                        <  2:342594/140‑1 (MQ=255)                                                                                          ccTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCAt                                    <  1:38962/140‑1 (MQ=255)                                                                                          ccTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCAt                                    <  1:941240/140‑1 (MQ=255)                                                                                          ccTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCAt                                    <  2:1433006/140‑1 (MQ=255)                                                                                            ttGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATAATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTa                                  <  2:940458/140‑1 (MQ=255)                                                                                             tgtgAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTAc                                 >  2:1211077/1‑140 (MQ=255)                                                                                                  gTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACa                            <  2:267598/140‑1 (MQ=255)                                                                                                     aTTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAggg                         >  2:879871/1‑140 (MQ=255)                                                                                                     aTTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAggg                         <  1:1211077/140‑1 (MQ=255)                                                                                                      ttttGATTAATGGTAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGgt                        <  2:1204436/140‑1 (MQ=255)                                                                                                                   tAGCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATGCCCa           <  1:650214/140‑1 (MQ=255)                                                                                                                     gCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACg         <  1:921769/140‑1 (MQ=255)                                                                                                                     gCGTCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACg         <  2:319143/140‑1 (MQ=255)                                                                                                                        tCGCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATCACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTa      >  2:542966/1‑140 (MQ=255)                                                                                                                          gCTTGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTAtt    <  1:1118200/140‑1 (MQ=255)                                                                                                                            ttGTCAATGTAAGCTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGa  <  2:1123390/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                               AGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGA  >  NC_000913/960081‑960343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑