Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,401,459	0	T	C	26.9%	142.6 / 23.4	78	D625G (GAT→GGT)	csrD	regulator of CsrB and CsrC decay
Reads supporting (aligned to +/- strand):  ref base T (45/12);  new base C (0/21);  total (45/33)
Fisher's exact test for biased strand distribution p-value = 6.49e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGAGCAGACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTC  >  NC_000913/3401322‑3401597                                                                                                                                          |                                                                                                                                           cGAGCAGACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATcc                                                                                                                                          >  1:1874261/1‑140 (MQ=255) cGAGCAGACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATcc                                                                                                                                          <  1:1894457/140‑1 (MQ=255)    gCAGACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCt                                                                                                                                       <  1:1756291/140‑1 (MQ=255)     cAGACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGATGCGAAAAAATCCCCtt                                                                                                                                      >  1:1299119/1‑140 (MQ=255)     cAGACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCtt                                                                                                                                      >  1:1916923/1‑140 (MQ=255)     cAGACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCtt                                                                                                                                      >  1:327010/1‑140 (MQ=255)      aGACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTg                                                                                                                                     <  1:1912600/140‑1 (MQ=255)      aGACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTg                                                                                                                                     >  2:139577/1‑140 (MQ=255)         cAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGccc                                                                                                                                  <  1:558497/140‑1 (MQ=255)         cAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGccc                                                                                                                                  <  1:1080004/140‑1 (MQ=255)         cAACCACACCCCCGTGAGACGCAGCGCGCATTATTCTACGTAAAAACGGATTAAACGGCAGGTTAAACCGAGTATTTTTGTGAATATTTTTTCCCGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAACCCCCTTGccc                                                                                                                                  <  2:1264057/140‑1 (MQ=255)           aGCACACTCCCCTGTGACGCAGCCCGCATTATTCAACGTGAAAGCGGATAAAACGGCAGGTTAAACCGGGTATTTTTGTAAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCAAAAAAACCCCCTTCCCCGc                                                                                                                                <  2:1207752/140‑1 (MQ=255)                        gagaCGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACcgcg                                                                                                                   <  1:995499/140‑1 (MQ=255)                        gagaCGCAGCGCGCATTATTCTACGTAAAAACGGATTAAACGGCGGGTTAAACCGAGTATCTTTGTGATTATTTTTTCGCGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAACCCCCTTGCCCGCCTGTAACACcgcg                                                                                                                   <  1:1766137/140‑1 (MQ=255)                        gagaCGCAGCGCGCATTATTCTACGTAAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTAAATATTTTTTCACGTTAGTATCAAGTGGTTGTGAGGACGCGAAAAAACCCCCTTGCCCGCCTGTAACACcgcg                                                                                                                   <  2:924948/140‑1 (MQ=255)                        gaaaCGCAGCGCGCATTTTTCTAGGTGAAAATGAATTAATCGAAAGGTTAACCAGTGTCTCTTTGTAAATGTTTTTCCGCGTCAGTATCAGGGGGGTGTGAGGGCGAAAAAAAACCCCCTTGCCCGCCTGTAACACcgcg                                                                                                                   <  2:1874261/137‑1 (MQ=255)                            cGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGa                                                                                                               >  2:251452/1‑140 (MQ=255)                            cGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGa                                                                                                               >  2:1065976/1‑140 (MQ=255)                              cAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAAt                                                                                                             >  2:91543/1‑140 (MQ=255)                              cAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAAt                                                                                                             >  1:1921272/1‑140 (MQ=255)                                 cgcgcATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAg                                                                                                          >  1:1541790/1‑140 (MQ=255)                                 cgcgcATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAg                                                                                                          >  1:1411778/1‑140 (MQ=255)                                             tACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTc                                                                                              >  1:573489/1‑140 (MQ=255)                                             tACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTc                                                                                              >  1:750414/1‑140 (MQ=255)                                             tACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTc                                                                                              >  2:952424/1‑140 (MQ=255)                                               cGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGc                                                                                            >  1:1461893/1‑140 (MQ=255)                                                gTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCt                                                                                           >  1:153079/1‑140 (MQ=255)                                                gTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCt                                                                                           >  1:1779588/1‑140 (MQ=255)                                                    aaaCGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTcgc                                                                                       >  2:591720/1‑140 (MQ=255)                                                    aaaCGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTcgc                                                                                       >  2:1186597/1‑140 (MQ=255)                                                                cGGCAGGTAAACCGGATTATCTTTGTAAATTTTTTTTCACGTTAGTATCAAGTGGTTGTGAGGACGCAAAAAACCCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCg                                                                           <  2:1125317/140‑1 (MQ=255)                                                                 ggCAGGTTAAACCGATTATTTTTGTAAATTTTTTTTCACGTTAGTATAAAGTGGCTGTGGGGCCGCAAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCgg                                                                          <  2:573489/140‑1 (MQ=255)                                                                   cAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTg                                                                        >  2:1950795/1‑140 (MQ=255)                                                                   cAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTg                                                                        >  1:705393/1‑140 (MQ=255)                                                                     ggTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCAAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGc                                                                      <  1:1220938/140‑1 (MQ=255)                                                                      gTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCg                                                                     >  1:1338438/1‑140 (MQ=255)                                                                        tAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTa                                                                   <  1:35308/140‑1 (MQ=255)                                                                        tAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCAAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTa                                                                   <  1:1056175/140‑1 (MQ=255)                                                                        tAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGGGAGGCCGCGAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTa                                                                   <  2:318473/140‑1 (MQ=255)                                                                        aaacccGAGTATCTTTGTAAATATTTTTTCACTTTAGTATCAAGTGGCTGTGAGGACGAAAAAAAACCCCCTTCCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTa                                                                   <  2:1411778/136‑1 (MQ=255)                                                                        aaaaCCGAGTATTTTTGTGAATTTTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTa                                                                   <  2:1327629/139‑1 (MQ=255)                                                                               aGTATTTTTGTAAATTTTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTGGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTgg                                                            <  2:616255/140‑1 (MQ=255)                                                                               aGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTgg                                                            >  2:256144/1‑140 (MQ=255)                                                                               aGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTgg                                                            >  1:1079844/1‑140 (MQ=255)                                                                                gTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTggg                                                           >  1:1409537/1‑140 (MQ=255)                                                                                    ttttGTAAATTTTTTTTCAGGTTAGTATCAAGTGGCTGTGAGGCCGCGAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCt                                                       <  2:1641215/139‑1 (MQ=255)                                                                                    ttttGTAAATTTTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCAAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCt                                                       <  2:1828245/139‑1 (MQ=255)                                                                                          aaaTTTTTTTTCACGTTAGTATAAAGTGGCTGTGAGGACGCGAAAAAACCCCCTTGCCCGCCTGTACCACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTccc                                                 <  2:705393/139‑1 (MQ=255)                                                                                             tatTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCCCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGa                                              >  2:195154/1‑140 (MQ=255)                                                                                               ttttttCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGc                                            <  1:251452/140‑1 (MQ=255)                                                                                               ttttttCACGTTAGTATAAAGGGGCTGGGAGGACGCGAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGc                                            <  2:1288337/140‑1 (MQ=255)                                                                                                  tttCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAgg                                         >  1:1951830/1‑140 (MQ=255)                                                                                                   ttCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGc                                        <  1:1186597/140‑1 (MQ=255)                                                                                                     cACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCtt                                      >  1:189496/1‑140 (MQ=255)                                                                                                          tattataAAGGGGCTGTGAGGCCGCAAAAAACCCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTccacc                                 <  1:402348/137‑1 (MQ=255)                                                                                                          tAGTATCAAGTGGTTGTGAGGACGCAAAAAACCCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTccacc                                 <  2:1338438/140‑1 (MQ=255)                                                                                                          tAGTATAAAGTGGCTGGGAGGACGCGAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTccacc                                 <  2:327010/140‑1 (MQ=255)                                                                                                          aattaaaaaGTGGTTGAGAGGCCGCAAAAAACCCCCCTCCCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTccacc                                 <  2:1916923/133‑1 (MQ=255)                                                                                                           aGTATCAAGTGGCTGTGAGGACGCGAAAAAACCCCCTGGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTccacca                                <  1:762323/140‑1 (MQ=255)                                                                                                           aGTATAAGGTGGCTGTGAGGACGGAAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTccacca                                <  2:1541790/140‑1 (MQ=255)                                                                                                           aGTACCAAGTGGCTGTGAGGCCGCAAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTccacca                                <  2:1079844/140‑1 (MQ=255)                                                                                                               tCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCt                            >  2:1232743/1‑140 (MQ=255)                                                                                                                  aGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTg                         >  2:1723169/1‑140 (MQ=255)                                                                                                                    tGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGaa                       >  1:1153896/1‑140 (MQ=255)                                                                                                                     ggCTGTGAGGACGCGAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAc                      <  1:1108381/140‑1 (MQ=255)                                                                                                                      gCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc                     >  2:714674/1‑140 (MQ=255)                                                                                                                      gCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc                     >  2:898454/1‑140 (MQ=255)                                                                                                                      gCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc                     >  2:1176983/1‑140 (MQ=255)                                                                                                                      gCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc                     >  1:1889662/1‑140 (MQ=255)                                                                                                                      gCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc                     >  2:170726/1‑140 (MQ=255)                                                                                                                      gCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc                     >  1:602148/1‑140 (MQ=255)                                                                                                                      gCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCAGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc                     >  1:1341020/1‑140 (MQ=255)                                                                                                                       cTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACca                    >  2:1395241/1‑140 (MQ=255)                                                                                                                       cTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACca                    >  2:915828/1‑140 (MQ=255)                                                                                                                         gtgAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACcagc                  <  1:1395241/140‑1 (MQ=255)                                                                                                                          tgAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACcagca                 >  1:1374601/1‑140 (MQ=255)                                                                                                                          tgAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTACTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACcagca                 >  1:112050/1‑140 (MQ=255)                                                                                                                              gACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTg             >  2:731727/1‑140 (MQ=255)                                                                                                                              gACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTg             >  1:990825/1‑140 (MQ=255)                                                                                                                                 gcgAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGtt          >  1:1422181/1‑140 (MQ=255)                                                                                                                                 gcgAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGtt          >  2:1442890/1‑140 (MQ=255)                                                                                                                                   aaaaaaaCCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTtct        <  2:990825/139‑1 (MQ=255)                                                                                                                                         aTCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTc  <  2:1461893/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                           CGAGCAGACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTC  >  NC_000913/3401322‑3401597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑