Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,429,9840TG24.5% 206.0 / 31.1 98L41V (TTG→GTG) cycAserine/alanine/glycine/cycloserine:H(+)symporter
Reads supporting (aligned to +/- strand):  ref base T (15/59);  new base G (24/0);  total (39/59)
Fisher's exact test for biased strand distribution p-value = 5.47e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 122 total aligned reads displayed.

GGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCT  >  NC_000913/4429845‑4430121
                                                                                                                                            |                                                                                                                                          
ggTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGgt                                                                                                                                            <  2:651424/140‑1 (MQ=255)
ggTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGgt                                                                                                                                            <  1:94421/140‑1 (MQ=255)
ggTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGgt                                                                                                                                            <  1:138478/140‑1 (MQ=255)
ggTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGgt                                                                                                                                            <  2:150096/140‑1 (MQ=255)
  tACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGgttg                                                                                                                                          >  1:1822384/1‑140 (MQ=255)
  tACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGGACGGGggtg                                                                                                                                          >  2:1348084/1‑140 (MQ=255)
       gaagaaAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGGCCATTGG‑ACGGGGTTGttttt                                                                                                                                     >  2:1957484/1‑138 (MQ=255)
       gaagaaAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTTGTGAGGGGGTGttttt                                                                                                                                     >  2:1834974/1‑138 (MQ=255)
       gaagaaAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTTGGACGGGGGTGTTTAt                                                                                                                                     >  2:1061200/1‑140 (MQ=255)
       gaagaaAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTAt                                                                                                                                     >  1:255130/1‑140 (MQ=255)
       gaagaaAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGGACGGGGGTGttttt                                                                                                                                     >  1:1656164/1‑138 (MQ=255)
         agaaAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATgg                                                                                                                                   >  2:989444/1‑140 (MQ=255)
           aaaaaaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCCTTGGTACGGGGGTGTTTTTgggg                                                                                                                                 >  2:773222/1‑140 (MQ=255)
           aaaaaaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATgggg                                                                                                                                 <  2:851122/140‑1 (MQ=255)
           aaaaaaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGGACGGGGGTGTTTTTgggg                                                                                                                                 >  2:358067/1‑140 (MQ=255)
            aaaaaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGt                                                                                                                                <  2:1428164/140‑1 (MQ=255)
               aaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGGTGTTTATGGGGTCTg                                                                                                                             >  1:1214105/1‑140 (MQ=255)
                   aTGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCaa                                                                                                                         <  1:1534305/140‑1 (MQ=255)
                   aTGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCaa                                                                                                                         <  1:1106585/140‑1 (MQ=255)
                   aTGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑ACCATTGGTACGGGGTTGTTTATGGGGTCTGGCaa                                                                                                                         <  1:513928/140‑1 (MQ=255)
                         gATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGAt                                                                                                                   <  1:381961/140‑1 (MQ=255)
                         gATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGAt                                                                                                                   <  1:1061200/140‑1 (MQ=255)
                          aTCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGGTGTTTTTGGGGGCTGGCAAAACGAtt                                                                                                                  >  1:356793/1‑140 (MQ=255)
                          aTCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATACTCAGCTTATTGCCATTGGCGGG‑GCCATTGGTACGGGGGTGGTTATGGGGGCTGGCAAAACGAtt                                                                                                                  >  2:606248/1‑140 (MQ=255)
                            cAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCCTTGGTACGGGGGTGTTTATGGGGGCTGGCAAAACGATTaa                                                                                                                >  1:1022335/1‑139 (MQ=255)
                            cAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGGACGGGGGTGTTTATGGGGTCTGGCAAAACGATTAg                                                                                                                >  1:671052/1‑140 (MQ=255)
                              ggTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCCTTTGGTCGGGGGTGTTTTTGGGGGCTGGCAAAAAGATTAG‑cc                                                                                                             >  2:916619/1‑140 (MQ=255)
                               gTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAATAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGGTGTTTATGGGGGGTGGCAAAACGATTAG‑ccc                                                                                                            >  2:707094/1‑139 (MQ=255)
                                tAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGGACGGGGGTGTTTTTGGGGTCTCGCCAAACGATTAG‑CCtt                                                                                                           >  2:648220/1‑140 (MQ=255)
                                 aaaaGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGGTGTTTATTGGGGCTGGGAAAAAAATTAG‑CCttt                                                                                                          >  2:381961/1‑139 (MQ=255)
                                   aaGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGGTGTTTTTGGGGTCTGGCAAAAAGATTAG‑CCTTGcc                                                                                                        >  2:1138749/1‑140 (MQ=255)
                                    aGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGGACGGGGGTGTTTTTGGGGGCTGGCAAAAAGATTAG‑CCTTGCCg                                                                                                       >  2:1009733/1‑140 (MQ=255)
                                     gTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCgg                                                                                                      <  2:186359/140‑1 (MQ=255)
                                      tCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCggg                                                                                                     <  1:719247/140‑1 (MQ=255)
                                       cGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGc                                                                                                    <  2:467251/140‑1 (MQ=255)
                                        gTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGGTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGcc                                                                                                   >  1:1267594/1‑140 (MQ=255)
                                         ttGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGGTGTTTATTGGGGCTGGCAAAACGATTAG‑CCTTGCCGGGGCg                                                                                                  >  2:34496/1‑140 (MQ=255)
                                         ttGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGGTGTTTATGGGGGCTGGCAAAACGATTAG‑CCTTGCCGGGCCg                                                                                                  >  1:1015172/1‑140 (MQ=255)
                                          tGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGt                                                                                                 <  2:1235129/140‑1 (MQ=255)
                                          tGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGt                                                                                                 <  1:916619/140‑1 (MQ=255)
                                           gCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTc                                                                                                <  1:1355269/140‑1 (MQ=255)
                                                 gatCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCAt                                                                                          <  2:1214105/140‑1 (MQ=255)
                                                 gatCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCAt                                                                                          <  2:1822384/140‑1 (MQ=255)
                                                  atCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCAtt                                                                                         <  2:61320/140‑1 (MQ=255)
                                                   tCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCAttt                                                                                        <  2:686942/140‑1 (MQ=255)
                                                   tCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCAttt                                                                                        <  1:407254/140‑1 (MQ=255)
                                                      ggCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCg                                                                                     <  1:648220/140‑1 (MQ=255)
                                                       gCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGt                                                                                    <  1:1861988/140‑1 (MQ=255)
                                                       gCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGt                                                                                    <  1:1316083/140‑1 (MQ=255)
                                                       gCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGGTGTTTTTGGGGGCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGt                                                                                    >  1:1589886/1‑140 (MQ=255)
                                                        cTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGtt                                                                                   <  1:1278752/140‑1 (MQ=255)
                                                        cTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGtt                                                                                   <  1:1957484/140‑1 (MQ=255)
                                                          ccGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGGTGTTTATGGGGGCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTta                                                                                 >  1:768323/1‑140 (MQ=255)
                                                           cGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTtat                                                                                <  1:358067/140‑1 (MQ=255)
                                                           cGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTtat                                                                                <  1:1666943/140‑1 (MQ=255)
                                                           cGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTtat                                                                                <  1:1804402/140‑1 (MQ=255)
                                                            ggCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTTGTACGGGGGTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCCATCATTTTCGTTtata                                                                               >  1:1078602/1‑140 (MQ=255)
                                                            ggCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGGTGTTTTTTGGGGCTGGCAAAACGATTAG‑CCTTTCCGGGGCGTCGGTCATTTTTGTTttta                                                                               >  2:432907/1‑140 (MQ=255)
                                                             gCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTatat                                                                              <  2:255130/140‑1 (MQ=255)
                                                                gAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGAt                                                                           <  1:989444/140‑1 (MQ=255)
                                                                 aaCAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCCTTGGGACGGGGTTGTTTATGGGGGCTGGCAAAAAGATTAG‑CCTTGCCGGGGCGGCGGTCATTTTTGTTTATATGATc                                                                          >  2:1555591/1‑140 (MQ=255)
                                                                 aaCAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGGACGGGGGTGTTTTTGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTTGTTTATATGATc                                                                          >  2:991768/1‑140 (MQ=255)
                                                                   cAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCAt                                                                        <  2:1319620/140‑1 (MQ=255)
                                                                   cAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCAt                                                                        <  2:838797/140‑1 (MQ=255)
                                                                   cAGTCGCTACGGCGCAATCTCACAAACCGACATACTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCAt                                                                        <  1:606248/140‑1 (MQ=255)
                                                                       cGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGt                                                                    <  2:437207/140‑1 (MQ=255)
                                                                            cGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTAt                                                               <  2:1179937/140‑1 (MQ=255)
                                                                             ggCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATg                                                              <  1:1834974/140‑1 (MQ=255)
                                                                              gcgcAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTTTGGGGTCTGGCAAAAAGATTAG‑CCTTTCCGGGCCGTCGATCATTTTCGTTTTTATGATCATTGGTTTTTTGc                                                             >  2:1336795/1‑140 (MQ=255)
                                                                               cgcAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGct                                                            <  1:1009733/140‑1 (MQ=255)
                                                                               cgcAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGct                                                            <  2:768323/140‑1 (MQ=255)
                                                                                    tctcACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCCTTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCtttt                                                       >  1:213528/1‑140 (MQ=255)
                                                                                      tcACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTc                                                     <  1:1718158/140‑1 (MQ=255)
                                                                                          aaaCCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGa                                                 <  1:888183/140‑1 (MQ=255)
                                                                                             ccGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGc                                              <  1:432907/140‑1 (MQ=255)
                                                                                              cGACATATTCAGCTTATTGCCATTGGCGGT‑GTCTTTCGGACGGGGGTGTTTATAGGGGGTTGGAAAACGAATCGTCCTTGCC‑GGCCGTCGCTCATTTTCCTTTTTATTCTGCTTGGGTTTATTCTCTTTTTTGGGATGCg                                             >  2:489726/1‑140 (MQ=255)
                                                                                              cGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGGTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTTGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCg                                             >  1:634719/1‑140 (MQ=255)
                                                                                              cGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGGACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCg                                             >  1:1088205/1‑140 (MQ=255)
                                                                                                aCATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGGTGTTTTTTGGGTCTGGCAAAAAGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCggg                                           >  1:1129291/1‑140 (MQ=255)
                                                                                                   tatTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCaa                                        <  1:773222/140‑1 (MQ=255)
                                                                                                    atTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAAt                                       <  1:211166/140‑1 (MQ=255)
                                                                                                      tCAGCTTGTTGCCATTGGCGGT‑GCCATTGGTACGGGGGTGTTTATGGGGGCTGGCAAAAAGATTAG‑CCTTTCCGGGCCGTCGATCATTTTTGTTTTTATGATCATTGGGTTTTTGCTCTTTTTCGTGATGCGGGCAATgg                                     >  2:78282/1‑140 (MQ=255)
                                                                                                          cTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGGTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGa                                 >  1:586338/1‑140 (MQ=255)
                                                                                                              ttGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATtg                             <  1:34496/140‑1 (MQ=255)
                                                                                                              ttGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATtg                             <  2:677483/140‑1 (MQ=255)
                                                                                                               tGCCATTGGCGGT‑GCCATTGGTACGGGGGTGTTTATGGGGGCTGGCAAAACGATTAG‑CCTTGCCGGGGCGTCGATCATTTTTGTTTATATGATCATTGGTTTTATGCTCTTTTTTGTGATGCGGGCAATGGGGGGATtgt                            >  2:1307726/1‑139 (MQ=255)
                                                                                                                  cATTGGCGGT‑GCCATTGGTACGGGGGTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTTGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATtgctgc                         >  1:1314344/1‑140 (MQ=255)
                                                                                                                    ttGGCGGT‑GCCATTGGTACGGGGGTGTTTATGGGGGCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGGTCATTTTCGTTTATATGATCATTGGGTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGGATTGCTGCtt                       >  1:2088/1‑140 (MQ=255)
                                                                                                                           t‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATc                <  1:1764941/140‑1 (MQ=255)
                                                                                                                                  tGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAAt          >  1:1144278/1‑140 (MQ=255)
                                                                                                                                    gTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATAc        <  1:1555591/140‑1 (MQ=255)
                                                                                                                                      aCGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACaa      <  2:620367/140‑1 (MQ=255)
                                                                                                                                        ggggTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTg              >  1:906756/1‑130 (MQ=255)
                                                                                                                                        ggggTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTg              <  2:906756/130‑1 (MQ=255)
                                                                                                                                        ggggTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAAt    >  1:1692711/1‑140 (MQ=255)
                                                                                                                                        ggggTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAAt    >  2:69495/1‑140 (MQ=255)
                                                                                                                                        ggggTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAAt    <  2:213528/140‑1 (MQ=255)
                                                                                                                                        ggggTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGAAATTGCTGCTTTCGAATCTGGAATACAAAt    <  2:1656164/140‑1 (MQ=255)
                                                                                                                                          ggTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCt  >  2:1428595/1‑140 (MQ=255)
                                                                                                                                          ggTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCt  >  1:473366/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                          
GGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAG‑CCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCT  >  NC_000913/4429845‑4430121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑