Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,196,164	A→T	D622V (GAT→GTT)	metG →	methionine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,196,164	0	A	T	100.0%	265.1 / NA	79	D622V (GAT→GTT)	metG	methionine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (34/45);  total (34/45)

CGCTAAAGTTGACCTGCGCGTGGCGCTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGATCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATCTCTGAAGGCATGGTGATGGCTGCCGGTCCTGGCGGGAAAGATATTTTCCTGCTAAGCCCGGA  >  NC_000913/2196027‑2196299                                                                                                                                          |                                                                                                                                        cGCTAAAGTTGACCTGCGCGTGGCGCTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTc                                                                                                                                       >  1:1517703/1‑140 (MQ=255)   cTAAAGTTGACCTGCGCGTGGCGCTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCg                                                                                                                                     <  2:1853797/140‑1 (MQ=255)    tAAAGTTGACCTGCGCGTGGCGCTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGc                                                                                                                                    >  2:346288/1‑140 (MQ=255)       aGTTGACCTGCGCGTGGCGCTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAgg                                                                                                                                 <  2:1059459/140‑1 (MQ=255)       aGTTGACCTGCGCGTGGCGCTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTACGCAgg                                                                                                                                 <  1:165562/140‑1 (MQ=255)         ttGACCTGCGCGTGGCGCTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCa                                                                                                                               <  2:8931/140‑1 (MQ=255)           gACCTGCGCGTGGCGCTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACt                                                                                                                             <  1:1254667/140‑1 (MQ=255)           gACCTGCGCGTGGCGCTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACt                                                                                                                             >  1:1514280/1‑140 (MQ=255)            aCCTGCGCGTGGCGCTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTg                                                                                                                            >  1:1014953/1‑140 (MQ=255)            aCCTGCGCGTGGCGCTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTg                                                                                                                            >  1:602386/1‑140 (MQ=255)            aCCTGCGCGTGGCGCTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTg                                                                                                                            >  1:1594080/1‑140 (MQ=255)             ccTGCGCGTGGCGCTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGa                                                                                                                           <  2:1594080/140‑1 (MQ=255)             ccTGCGCGTGGCGCTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGa                                                                                                                           <  1:178410/140‑1 (MQ=255)                 cgcgTGGCGCTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTgg                                                                                                                       >  1:70499/1‑140 (MQ=255)                 cgcgTGGCGCTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTAACGCTGGATCTCGGCGGTGAAAAACGCAATTGCTTCTCCGGTATTCGTTCTGCTTACCCGGTTTCGCAGGCACTGATTgg                                                                                                                       >  2:533270/1‑140 (MQ=255)                 cgcgTGGCGCAGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGCAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTgg                                                                                                                       >  2:668546/1‑140 (MQ=255)                  gcgTGGCGCTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGgt                                                                                                                      >  2:749962/1‑140 (MQ=255)                  gcgTGGCGCTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGgt                                                                                                                      <  2:1178836/140‑1 (MQ=255)                  gcgTGGCGCTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGgt                                                                                                                      <  1:338931/140‑1 (MQ=255)                    gTGGCGCTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGgtcg                                                                                                                    <  1:1057954/140‑1 (MQ=255)                    gTGGCGCTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGgtcg                                                                                                                    <  1:497484/140‑1 (MQ=255)                     tGGCGCTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGgtcgt                                                                                                                   <  1:1958385/140‑1 (MQ=255)                     tGGCGCTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGgtcgt                                                                                                                   <  2:1221391/140‑1 (MQ=255)                     tGGCGCTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGgtcgt                                                                                                                   <  1:660789/140‑1 (MQ=255)                      ggCGCTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGGATTCGTTCTGCTTTCCCGGTTCCGCAGGCACTGATTGgtcgtc                                                                                                                  >  2:1120/1‑140 (MQ=255)                       gcgcTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTca                                                                                                                 <  1:1890132/140‑1 (MQ=255)                       gcgcTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTca                                                                                                                 <  1:22736/140‑1 (MQ=255)                       gcgcTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTca                                                                                                                 <  1:346288/140‑1 (MQ=255)                       gcgcTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTca                                                                                                                 <  2:1656400/140‑1 (MQ=255)                       gcgcTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTca                                                                                                                 <  1:86574/140‑1 (MQ=255)                       gcgcTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTca                                                                                                                 <  1:76922/140‑1 (MQ=255)                       gcgcTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTca                                                                                                                 <  2:553016/140‑1 (MQ=255)                        cgcTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTcac                                                                                                                >  1:742195/1‑140 (MQ=255)                           tGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACAcc                                                                                                             >  2:1189755/1‑140 (MQ=255)                            gATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCa                                                                                                            <  1:1877794/140‑1 (MQ=255)                                  aaaCGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTAtgg                                                                                                      >  1:1167396/1‑140 (MQ=255)                                      gCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGc                                                                                                  <  2:1514280/140‑1 (MQ=255)                                      gCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGc                                                                                                  <  1:1636867/140‑1 (MQ=255)                                       cAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCGCACCATTATGGTGGCt                                                                                                 >  2:1061149/1‑140 (MQ=255)                                       cAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCt                                                                                                 <  1:852814/140‑1 (MQ=255)                                       cAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCt                                                                                                 >  1:685025/1‑140 (MQ=255)                                       cAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCt                                                                                                 >  1:144924/1‑140 (MQ=255)                                       cAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGAAATGGCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCt                                                                                                 >  2:1601488/1‑140 (MQ=255)                                               gttgAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGc                                                                                         <  2:123870/140‑1 (MQ=255)                                                ttgAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGca                                                                                        <  2:742195/140‑1 (MQ=255)                                                 tgAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGcac                                                                                       <  2:1435485/140‑1 (MQ=255)                                                    aGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGcaccac                                                                                    >  2:1742586/1‑140 (MQ=255)                                                    aGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGcaccac                                                                                    >  2:130010/1‑140 (MQ=255)                                                             cAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATgc                                                                           <  1:1189755/140‑1 (MQ=255)                                                                 ctgctgCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCtt                                                                       >  1:1809994/1‑140 (MQ=255)                                                                    ctgcGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCgg                                                                    <  2:70499/140‑1 (MQ=255)                                                                     tgcGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGt                                                                   <  2:732575/140‑1 (MQ=255)                                                                      gcgcCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTa                                                                  <  1:1539406/140‑1 (MQ=255)                                                                      gcgcCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTa                                                                  <  2:614234/140‑1 (MQ=255)                                                                           tGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATctct                                                             <  2:1954421/140‑1 (MQ=255)                                                                           tGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATctct                                                             <  1:749962/140‑1 (MQ=255)                                                                             aCGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATCTCTGa                                                           <  2:413791/140‑1 (MQ=255)                                                                             aCGCTGGATCTCGGCGGTGAAAAACGAAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGACACCACGTAAAATGCGCTTCGGTATCTCTGa                                                           <  1:1601488/140‑1 (MQ=255)                                                                                 tGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATCTCTGAAGGc                                                       <  2:1564269/140‑1 (MQ=255)                                                                                 tGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATCTCTGAAGGc                                                       <  2:1768261/140‑1 (MQ=255)                                                                                        cggcggTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATCTCTGAAGGCATGGTGa                                                >  2:555141/1‑140 (MQ=255)                                                                                        cggcggTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATCTCTGAAGGCATGGTGa                                                >  1:996786/1‑140 (MQ=255)                                                                                             gTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATCTCTGAAGGCATGGTGATGGCt                                           >  2:160001/1‑140 (MQ=255)                                                                                             gTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATCTCTGAAGGCATGGTGATGGCt                                           >  1:1439859/1‑140 (MQ=255)                                                                                                         aTGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATCTCTGAAGGCATGGTGATGGCTGCCGGTCCTGGc                               <  1:130010/140‑1 (MQ=255)                                                                                                          tGTCTTCTCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATCTCTGAAGGCATGGTGATGGCTGCCGGTCCTGGCg                              >  2:1044617/1‑140 (MQ=255)                                                                                                             cttctCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATCTCTGAAGGCATGGTGATGGCTGCCGGTCCTGGCGGGa                           <  2:1167396/140‑1 (MQ=255)                                                                                                              ttctCCGGTATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATCTCTGAAGGCATGGTGATGGCTGCCGGTCCTGGCGGGaa                          <  2:1809994/140‑1 (MQ=255)                                                                                                                    ggTATTCGTTCTGCTTACCCGGTTCCACAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATCTCTGAAGGCATGGTGATGGCTGCCGGTCCTGGCGGGAAAGatat                    >  2:726209/1‑140 (MQ=255)                                                                                                                      tATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATCTCTGAAGGCATGGTGATGGCTGCCGGTCCTGGCGGGAAAGGTAttt                  >  2:1100038/1‑140 (MQ=255)                                                                                                                      tATTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATCTCTGAAGGCATGGTGATGGCTGCCGGTCCTGGCGGGAAAGATAttt                  >  1:1273201/1‑140 (MQ=255)                                                                                                                       aTTCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATCTCTGAAGGCATGGTGATGGCTGCCGGTCCTGGCGGGAAAGATAtttt                 <  1:1044617/140‑1 (MQ=255)                                                                                                                        ttCGTTCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATCTCTGAAGGCATGGTGATGGCTGCCGGTCCTGGCGGGAAAGATATTTTc                <  1:579861/140‑1 (MQ=255)                                                                                                                             tCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATCTCTGAAGGCATGGTGATGGCTGCCGGTCCTGGCGGGAAAGATATTTTCCTGCt           >  2:1573989/1‑140 (MQ=255)                                                                                                                             tCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATCTCTGAAGGCATGGTGATGGCTGCCGGTCCTGGCGGGAAAGATATTTTCCTGCt           >  2:1947702/1‑140 (MQ=255)                                                                                                                             tCTGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATCTCTGAAGGCATGGTGATGGCTGCCGGTCCTGGCGGGAAAGATATTTTCCTGCt           >  2:1867015/1‑140 (MQ=255)                                                                                                                               tGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATCTCTGAAGGCATGGTGATGGCTGCCGGTCCTGGCGGGAAAGATATTTTCCTGCTaa         >  2:1186394/1‑140 (MQ=255)                                                                                                                               tGCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATCTCTGAAGGCATGGTGATGGCTGCCGGTCCTGGCGGGAAAGATATTTTCCTGCTaa         >  2:1314730/1‑140 (MQ=255)                                                                                                                                gCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGTACCACGTAAAATGCGCTTCGGTATCTCTGAAGGCATGGTGATGGCTGCCGGTCCTGGCGGGGAAGATATTTTCCTGCTAAg        >  2:345169/1‑140 (MQ=255)                                                                                                                                gCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATCTCTGAAGGCATGGTGATGGCTGCCGGTCCTGGCGGGAAAGATATTTTCCTGCTAAg        >  1:1564686/1‑140 (MQ=255)                                                                                                                                gCTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATCTCTGAAGGCATGGTGATGGCTGCCGGTCCTGGCGGGAAAGATATTTTCCTGCTAAg        >  1:1235194/1‑140 (MQ=255)                                                                                                                                 cTTACCCGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATCTCTGAAGGCATGGTGATGGCTGCCGGTCCTGGCGGGAAAGATATTTTCCTGCTAAGc       <  1:1742586/140‑1 (MQ=255)                                                                                                                                      ccGGTTCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATCTCTGAAGGCATGGTGATGGCTGCCGGTCCTGGCGGGAAAGATATTTTCCTGCTAAGCCCGGa  >  1:329864/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        CGCTAAAGTTGACCTGCGCGTGGCGCTGATTGAAAACGCAGAGTTTGTTGAAGGTTCTGACAAACTGCTGCGCCTGACGCTGGATCTCGGCGGTGAAAAACGCAATGTCTTCTCCGGTATTCGTTCTGCTTACCCGGATCCGCAGGCACTGATTGGTCGTCACACCATTATGGTGGCTAACCTGGCACCACGTAAAATGCGCTTCGGTATCTCTGAAGGCATGGTGATGGCTGCCGGTCCTGGCGGGAAAGATATTTTCCTGCTAAGCCCGGA  >  NC_000913/2196027‑2196299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑